DNA biosensor using fluorescence microscopy and impedance spectroscopy

Berdat, Daniel; Marin, Annick; Herrera, F.; Gijs, Martin A. M.

doi:10.1016/j.snb.2006.04.064

Cited by 39 publications

(30 citation statements)

References 22 publications

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“…[16][17][18] The presence of two succinimidyl groups in N,N′-disuccinimidyl suberate makes the combination of two amine groups in the system possible. By this reaction, Berdat et al 16 immobilized DNA on a silicon substrate; the immobilization was due to the reaction of silicon with the amine group in DNA. In the present work, the cross-linking reaction of DNA may have occurred in the presence of cellulose (filter paper).…”

Section: Materials and Preparation Of Dna-filter Hybridmentioning

confidence: 99%

Recovery and Separation of Rare Earth Elements Using Columns Loaded with DNA-filter Hybrid

et al. 2012

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Given that the supply of several rare earth elements (REEs) is sometimes limited, recycling REEs used in various advanced materials, such as Nd magnets, is important for realizing efficient use of REE resources. In the present work, the feasibility of using DNA for REE recovery and separation was examined, along with the identification of the binding site of REEs in DNA. In particular, a DNA-cellulose filter paper hybrid was prepared so that DNA-based materials can be used for the separation of REEs using columns loaded with DNA. N,N′-Disuccinimidyl was used as a cross-linker reagent for the fixation of DNA onto a fibrous cellulose filter. The results showed that (i) the DNA-filter hybrid has a sufficiently high affinity to adsorb REEs; (ii) the adsorption capacity was 0.182 mg/g for Nd; and (iii) the affinity of REEs for DNA was stronger for REEs with larger atomic numbers. The difference of the affinity among REEs in the third result was compared with the adsorption patterns of REEs discussed in the literature. The comparison suggests that phosphate in the DNA-filter paper hybrid was responsible for REE adsorption onto the hybrid. The results were supported by the Nd, Dy, and Lu LIII-edge EXAFS; the REE-P shell was identified for the second neighboring atom, showing the importance of the phosphate site as REE binding sites. The difference in the affinity among REEs suggest that group separation of REEs (such as La, Ce, (Pr and Nd), (Ho, Dy, and Er), (Tb and Gd), (Sm, Eu), Tm, Yb, and Lu) is possible, although complete isolation of each REE from a solution containing all REEs may be difficult. For practical applications, Nd and Fe(III) were successfully separated from a synthetic solution of Nd magnet waste using columns loaded with the DNAfilter hybrid.

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Section: Materials and Preparation Of Dna-filter Hybridmentioning

confidence: 99%

Recovery and Separation of Rare Earth Elements Using Columns Loaded with DNA-filter Hybrid

et al. 2012

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“…Such devices rely on the intimate coupling of a biological recognition element with a physical transducer to convert the biological signals into an electrical signal or other signals, proportional to the concentration of analytes [11][12][13][14][15][16]. Biosensors eliminate the need of the sample preparation and hence offer great promise for several on-site analytical applications of rapid and low cost measurements [17].…”

Section: Dna Biosensorsmentioning

confidence: 99%

DNA Biosensors-A Review

Kavita¹

2017

J Bioengineer & Biomedical Sci

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Recent advances in developing biosensors which are sensitive and specific have opened new opportunities for DNA biosensors. DNA biosensors, based on nucleic acid recognition methods, are being developed towards the assay of rapid, simple and economical testing of genetic and infectious diseases. Moreover, the detection of specific DNA sequence is of significance in numerous areas including clinical, environmental and food analysis. Advancements in technology like SELEX and SAM are being used to develop better detection methods (both Direct and Indirect detection) for DNA Biosensors. Unlike enzyme or antibodies, nucleic acid recognition layers can be readily synthesized and regenerated for multiple uses. DNA biosensors and gene chips are of major interest due to their great potential for obtaining sequence-specific information in a faster, simpler and cheaper manner compared to the traditional hybridization. Further, increase of interest to DNA based sensors can be expected in near future together with a commercial production of these devices and their extensive use. However, basic research is still necessary to improve the sensor technologies, sensing strategies as well as analytical instrumentations and procedures.

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“…sensory surface, SPR is able to detect thrombin with a concentration ranging from 0.08 to 200 nM. On the other hand, many label-free technologies are based on electrical detection of the intrinsic charges of biomolecules [9], or the impedance changes of an electrode-electrolyte interface due to the absorption or binding of biomolecules [10], or the conductance changes of nanomaterials due to the binding with biomolecules [11]. Among these technologies, electrochemical sensors with thrombin-aptamer complex are able to detect 5-35 nM thrombin without any amplification technology [8,12,13].…”

Section: Introductionmentioning

confidence: 99%

“…A floating gate formed by metals and polygates then couples potential changes from the sensor surface to the transistors. Compared to the first ion-sensitive field-effect transistor whose gate is replaced by an aqueous solution [10], the floating-gate structure is helpful for reducing sensor drifts induced by ion absorption, as well as the interference owing to photonic currents. However, the thick passivation layer reduces the coupling capacitance of the EGFET significantly.…”

Section: Introductionmentioning

confidence: 99%

Immobilized rolling circle amplification on extended-gate field-effect transistors with integrated readout circuits for early detection of platelet-derived growth factor

Lin¹,

Hsu

Yang³

et al. 2016

Anal Bioanal Chem

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Detection of tumor-related proteins with high specificity and sensitivity is important for early diagnosis and prognosis of cancers. While protein sensors based on antibodies are not easy to keep for a long time, aptamers (single-stranded DNA) are found to be a good alternative for recognizing tumor-related protein specifically. This study investigates the feasibility of employing aptamers to recognize the platelet-derived growth factor (PDGF) specifically and subsequently triggering rolling circle amplification (RCA) of DNAs on extended-gate field-effect transistors (EGFETs) to enhance the sensitivity. The EGFETs are fabricated by the standard CMOS technology and integrated with readout circuits monolithically. The monolithic integration not only avoids the wiring complexity for a large sensor array but also enhances the sensor reliability and facilitates massive production for commercialization. With the RCA primers immobilized on the sensory surface, the protein signal is amplified as the elongation of DNA, allowing the EGFET to achieve a sensitivity of 8.8 pM, more than three orders better than that achieved by conventional EGFETs. Moreover, the responses of EGFETs are able to indicate quantitatively the reaction rates of RCA, facilitating the estimation on the protein concentration. Our experimental results demonstrate that immobilized RCA on EGFETs is a useful, label-free method for early diagnosis of diseases related to low-concentrated tumor makers (e.g., PDGF) for serum sample, as well as for monitoring the synthesis of various DNA nanostructures in real time. Graphical Abstract The tumor-related protein, PDGF, is detected by immobilizing rolling circle amplification on an EGFET with integrated readout circuit.

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DNA biosensor using fluorescence microscopy and impedance spectroscopy

Cited by 39 publications

References 22 publications

Recovery and Separation of Rare Earth Elements Using Columns Loaded with DNA-filter Hybrid

Recovery and Separation of Rare Earth Elements Using Columns Loaded with DNA-filter Hybrid

DNA Biosensors-A Review

Immobilized rolling circle amplification on extended-gate field-effect transistors with integrated readout circuits for early detection of platelet-derived growth factor

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