DNA-catalyzed efficient production of single-stranded DNA nanostructures

Jia, Youli; Chen, Liman; Li, Wei; Gu, Hongzhou

doi:10.1016/j.chempr.2020.12.001

Cited by 36 publications

(48 citation statements)

References 69 publications

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“…For example, strategies based on polymerase chain reaction (PCR) amplification , and recombinant phagemid systems − were reported to produce long ssDNA scaffolds. Furthermore, functional nucleic acid sequence such as DNAzymes have also been inserted into the scaffolds to allow postfolding DNA origami domain separation. ,− …”

Section: Introductionmentioning

confidence: 99%

Aptamer-Integrated Scaffolds for Biologically Functional DNA Origami Structures

Chen

Jia

et al. 2021

ACS Appl. Mater. Interfaces

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The manufacture of DNA origami nanostructures with highly ordered functional motifs is of great significance for biomedical applications. Here, we present a robust strategy to produce customized scaffolds with integrated aptamer sequences, which enables direct construction of functional DNA origami structures. As we demonstrated, aptamers of various numbers and types were efficiently and stably integrated in userdefined positions of the scaffolds. Specifically, two different thrombin aptamer sequences were simultaneously inserted into the M13mp18 phage genome. The assembled functional DNA origami structures from this aptamer-integrated scaffold exhibited increased binding efficiency to thrombin and displayed more than 10-fold stronger resistance to exonuclease degradation than that produced using the traditional staple extension method. Additionally, a scaffold integrated with the platelet-derived growth factor aptamer was produced, and the assembled DNA origami structures showed significant inhibitory effect on breast cancer cells MDA-MB-231. This scalable method of creating design-specific scaffolds opens up a new way to construct more stable and functionally robust DNA origami structures and thus provides an important basis for their broader applications.

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Section: Introductionmentioning

confidence: 99%

Aptamer-Integrated Scaffolds for Biologically Functional DNA Origami Structures

Chen

Jia

et al. 2021

ACS Appl. Mater. Interfaces

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“…Taking advantage of DNA’s excellent programmability, we engineered TX1b nanoflowers and were able to utilize them as nanosponges to specifically adsorb Ni 2+ from artificial wastewater. Recent breakthroughs in biotechnology − have removed the hurdles in cost and scale of DNA synthesis. Thus, we believe that the Ni 2+ -binding DNA aptamers reported here have the potential as a novel and practical molecular tool for high-quality nickel manipulation in the future.…”

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confidence: 99%

Small DNAs that Bind Nickel(II) Specifically and Tightly

Zhang

Xia

et al. 2021

Anal. Chem.

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Metal recognition by nucleic acids provides an intriguing route for biosensing of metal. Toward this goal, a key prerequisite is the acquisition of nucleic acids that can selectively respond to specific metals. Herein, we report for the first time the discovery of two small DNAs that can specifically bind Ni 2+ and discriminate against similar ions, particularly, Co 2+ . Their minimal effective constructs are 60−70 nucleotides (nt) in length with Ni 2+ binding even at harsh denaturing conditions of 8 M urea and 50 mM EDTA. Using isothermal titration calorimetry (ITC), we estimated the dissociation constant (K D ) of a representative DNA to be 24.0 ± 4.5 μM, with a 9:1 stoichiometry of Ni 2+ bound to DNA. As being engineered into nanosized particles, these DNAs can act like nanosponges to specifically adsorb Ni 2+ from artificial wastewater, demonstrating their potential as a novel molecular tool for high-quality nickel enrichment and detection.

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“…This simple, time-and cost-effective technique can be This journal is © The Royal Society of Chemistry 2022 applied for amplification of higher-ordered, complex PX structures that can self-fold. 34 In addition, such enzymatic replication can also be performed with the use of helper phages infecting E. coli cells for enhanced amplification. 34,35 To make RCA and the cellular amplification even more affordable, catalytic DNAs or deoxyribozymes have been introduced as a cost-effective alternative to the restriction enzymes for cleaving/ extracting the amplified target single-stranded PX motifs in a large quantity.…”

Section: Scalable Enzymatic Amplification Of Px Motifsmentioning

confidence: 99%

“…34,35 To make RCA and the cellular amplification even more affordable, catalytic DNAs or deoxyribozymes have been introduced as a cost-effective alternative to the restriction enzymes for cleaving/extracting the amplified target single-stranded PX motifs in a large quantity. 34…”

Section: Introductionmentioning

confidence: 99%