DNA Cleavage by Chemically Exfoliated Molybdenum Disulfide Nanosheets

Zhao, Yingcan; Xu, Jiayi; Jiang, Xingyu

doi:10.1021/acs.est.1c00115

Cited by 10 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The AFM images showed a similar pattern with the topological rearrangement of pDNA as that reported in the literature. 49 The topological rearrangement of pDNA observed by AFM images as well as the results of agarose gel electrophoresis both demonstrated that MBPs exhibited Topo I simulated activity in the cleavage process. 51−53 Topo I contained in the human body has a positive charge, and its electrostatic interaction with DNA slows down the rotation of the cleaved DNA chain, which is beneficial for regulation of the topological rearrangement of DNA.…”

Section: ■ Results and Discussionmentioning

confidence: 87%

“…With the increase of temperature, the cleavage efficiency of MBP-f on pDNA was greatly improved (Figure a). Compared with other reported artificial nucleases, − MBP has significant advantages on the binding affinity and DNA cleavage activity and does not require exogenous reagents. High ion concentrations tend to destroy the protein structure of natural enzymes or affect the binding strength of DNA cleavage agents to DNA, resulting in decreased activity.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis and DNA Cleavage Activity of Cationic Methylene-Blue-Backboned Polymers

Cai

Zhang

et al. 2023

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Methods of DNA cleavage have broad bioapplications in gene editing, disease treatment, and biosensor design. The traditional method for DNA cleavage is mainly through oxidation or hydrolysis mediated by small molecules or transition metal complexes. However, DNA cleavage by artificial nucleases using organic polymers has been rarely reported. Methylene blue has been extensively studied in the fields of biomedicine and biosensing due to its excellent singlet oxygen yield, redox properties, and good DNA affinity. Methylene blue mainly relies on light and oxygen for DNA cleavage, and the cutting rate is slow. Here, we synthesize cationic methylene-blue-backboned polymers (MBPs) that can bind DNA efficiently and induce DNA cleavage through free radical mechanisms in the absence of light and exogenous reagents, showing high-efficiency nuclease activity. In addition, MBPs with different structures showed selectivity for DNA cleavage, and the cleavage efficiency of the flexible structure was significantly higher than that of the rigid structure. Studies on the DNA cleavage mechanism have shown that the cleavage mechanism of MBPs is not through the common ROS-mediated oxidative cleavage pathway, but through the radical of MBP• inducing DNA cleavage. Meanwhile, MBPs can simulate topoisomerase I (Topo I)-mediated topological rearrangement of superhelical DNA. This work paved a way for the application of MBPs in the field of artificial nucleases.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Synthesis and DNA Cleavage Activity of Cationic Methylene-Blue-Backboned Polymers

Cai

Zhang

et al. 2023

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

A Strategy of Killing Two Birds With One Stone for Blocking Drug Resistance Spread With Engineered Bdellovibrio bacteriovorus

Rao,

Wang,

Zhang

et al. 2024

Advanced Materials

View full text Add to dashboard Cite

Drug‐resistant pathogens significantly threaten human health and life. Simply killing drug‐resistant pathogens cannot effectively eliminate their threat since the drug‐resistant genes (DRGs) released from dead drug‐resistant pathogens are difficult to eliminate and can further spread via horizontal gene transfer, leading to the spread of drug resistance. The development of antibacterial materials with sterilization and DRGs cleavage activities is highly crucial. Herein, a living system, Ce‐PEA@Bdello, is fabricated with bacterial killing and DRGs cleavage activities for blocking bacterial drug resistance dissemination by engineered Bdellovibrio bacteriovorus (Bdello). Ce‐PEA@Bdello is obtained by engineering Bdello with dopamine and a multinuclear cerium (IV) complex. Ce‐PEA@Bdello can penetrate and eliminate kanamycin‐resistant P. aeruginosa (KanR) biofilms via the synergistic effect of predatory Bdello and photothermal polydopamine under near‐infrared light. Additionally, the DNase‐mimicking ability of Ce‐PEA@Bdello endows it with genome and plasmid DNA cleavage ability. An in vivo study reveals that Ce‐PEA@Bdello can eliminate P. aeruginosa (KanR) and cleave DRGs in scald/burn infected wounds to block the spread of drug resistance and accelerate wound healing. This bioactive system constructed from natural living materials offers a promising means for blocking the spread of drug resistance.

show abstract

DNA Cleavage System by Nanomaterials

Li,

Zhao

2024

ChemBioChem

View full text Add to dashboard Cite

DNA cleavage using specific agents is essential for gaining insights into the mechanisms of DNA breakage, repair, and signal transduction. These cleaving agents hold significant promise as therapeutic drugs in biomedical applications. Recently, the development of biomimetic nanozymes ‐ an innovative class of inorganic nanomaterials that exhibit enzyme‐like catalytic activity ‐ has paved the way for the creation of novel DNA cleavage systems. Nanomaterials have been utilized as DNA cleavage agents due to their exceptional catalytic activity and stability. In this review, we summarized the DNA cleavage activities and mechanisms with various nanomaterials. This review study will have great importance on designing the next‐generation “crispr” nanomaterials for more biomedical and environmental applications.

show abstract

DNA Cleavage by Chemically Exfoliated Molybdenum Disulfide Nanosheets

Cited by 10 publications

References 35 publications

Synthesis and DNA Cleavage Activity of Cationic Methylene-Blue-Backboned Polymers

Synthesis and DNA Cleavage Activity of Cationic Methylene-Blue-Backboned Polymers

A Strategy of Killing Two Birds With One Stone for Blocking Drug Resistance Spread With Engineered Bdellovibrio bacteriovorus

DNA Cleavage System by Nanomaterials

Contact Info

Product

Resources

About