DNA cytometry confirms the utility of the Bethesda system for the classification of Papanicolaou smears

Bollmann, R.; Bollmann, Magdolna; Henson, Donald E.; Bodó, M

doi:10.1002/cncr.9033.abs

Cited by 10 publications

(31 citation statements)

References 29 publications

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“…In addition, the positivity rate of flow cytometry increased with the severity of cervical cytology findings. Our results support the hypothesis that the amount of DNA detected by flow cytometry is correlated with the CIN stage [14, 15]. HR-HPV infection is known to be a necessary but not sufficient condition in cervical carcinogenesis [16, 17].…”

Section: Discussionsupporting

confidence: 79%

Evaluation of the LC-1000 Flow Cytometry Screening System for Cervical Cancer Screening in Routine Health Checkups

et al. 2018

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Objective: This study evaluated the performance of a flow cytometry system (LC-1000) in screening cervical precancerous lesions at routine health checkups. Study design: In total, 928 health examinees were enrolled at 16 health promotion centers in 13 Korean cities between 2016 and 2017. All participants underwent liquid-based cervical cytology and flow cytometry testing to determine the cell proliferation index (CPIx). Results: The positivity rate of the LC-1000 system increased with the severity of the cervical cytology findings (p for trend < 0.001). When low-grade squamous intraepithelial lesion (LSIL) or higher (including LSIL, high-grade squamous intraepithelial lesion [HSIL], and atypical squamous cells without excluding HSIL [ASC-H]) was defined as gold-standard positivity, the sensitivity, specificity, PPV, and NPV of LC-1000 were 75.3% (95% confidence interval [CI], 66.8–83.7), 58.5% (95% CI, 55.2–61.9), 18.1% (95% CI, 14.5–21.8), and 95.1% [95% CI, 93.2–97.0], respectively. The median CPIx increased significantly from normal cytology to HSIL (p < 0.001). The median CPIx was higher in high-risk human papillomavirus (HR-HPV)-positive cases than in HR-HPV-negative cases (0.23 vs. 0.17, p < 0.001), while it did not differ between HR-HPV-positive and HR-HPV-negative cases with normal cytology findings (0.16 vs. 0.16, p = 0.700). Conclusion: The LC-1000 system is potentially useful for screening cervical precancer and cancer, especially when excluding normal or ASC of undetermined significance cases in routinely screened populations.

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Section: Discussionsupporting

confidence: 79%

Evaluation of the LC-1000 Flow Cytometry Screening System for Cervical Cancer Screening in Routine Health Checkups

et al. 2018

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“…Our results did not show any diploid DNA histogram in cases with LSIL or HSIL. However, varying percentages of diploidy and/or aneuploidy have been reported in cervical preneoplastic lesions 6–8,10,21–28 . All 17 cases of CIN3 reported by Walsh et al.…”

Section: Discussionmentioning

confidence: 62%

“…In a recent study, Reich and Ballon 23 reported DNA aneuploidy in all 24 of their cases with biopsy‐proven CIN (five CIN1, six CIN2 and 13 CIN3). Other studies have reported the incidence of aneuploidy to vary between 14% and 75.5% in CIN1, 55% and 64.3% in CIN2 and 83.3% and 95.2% in CIN3 6–8,10,24–28 . HPV infection induces polyploidization in nuclear DNA, which may be an integral step in the evolution of aneuploidy and carcinogenesis 4,9,14,29 .…”

Section: Discussionmentioning

confidence: 99%

“…However, varying percentages of diploidy and ⁄ or aneuploidy have been reported in cervical preneoplastic lesions. [6][7][8]10,[21][22][23][24][25][26][27][28] All 17 cases of CIN3 reported by Walsh et al 21 had non-diploid DNA content; nine were aneuploid and eight were tetraploid. Multhaupt et al 22 reported DNA aneuploidy in all 12 of their cases of SIL.…”

Section: Discussionmentioning

confidence: 99%

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Diagnostic and prognostic significance of image cytometric DNA ploidy measurement in cytological samples of cervical squamous intraepithelial lesions

Demırel¹,

Akyürek²,

Ramzy³

2013

Cytopathology

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“…In addition, aneuploidy is frequently observed with atypia, which can be explained as an effect of HPV on replicating cells. Aneuploidy can be demonstrated in cervical cytological samples by DNA content measurements 10–12. Image analysis provides stoichiometric methods to measure total DNA content under microscopic conditions.…”

mentioning

confidence: 99%

Some sorption characteristics of poly(trimethylene terephthalate) with disperse dyes

Yang

Brown²,

2002

J of Applied Polymer Sci

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Sorption characteristics, such as sorption kinetics and isotherms, relation between dye sorption and shade development of poly(trimethylene terephthalate) (PTT) and their comparison with poly(ethylene terephthalate) (PET), were reported. Results from two different dyeing rate studies, dyeing rate under constant temperature and under changing temperature, were examined to obtain the common rate constants, and to obtain information for the design of dyeing temperature profile and holding time. Rate constants of PTT were compared with that of PET. Sorption isotherms of disperse dyes on PTT were also studied. Both Nernst and Langmuir models were examined. The dye uptake ability of PTT was compared with PET.

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DNA cytometry confirms the utility of the Bethesda system for the classification of Papanicolaou smears

Cited by 10 publications

References 29 publications

Evaluation of the LC-1000 Flow Cytometry Screening System for Cervical Cancer Screening in Routine Health Checkups

Evaluation of the LC-1000 Flow Cytometry Screening System for Cervical Cancer Screening in Routine Health Checkups

Diagnostic and prognostic significance of image cytometric DNA ploidy measurement in cytological samples of cervical squamous intraepithelial lesions

Some sorption characteristics of poly(trimethylene terephthalate) with disperse dyes

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