DNA Cytometry of Pure Dysgerminomas of the Ovary

Oud, Peter S.; Soeters, R. P.; Pahlplatz, M. M. M.; Hermkens, H. G.; Beck, H. L. M.; Reubsaet‐Veldhuizen, José A. M.; Vooijs, G. P.

doi:10.1097/00004347-198809000-00006

Cited by 17 publications

(6 citation statements)

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“…However, the expression levels of KIT were similar between the two types of germinomas. The karyotypes of the second type were near triploid or tetraploid, and these karyotypes were reported in adult GCTs 34 . Different from the germinomas in our cohort, most adult seminomas carried isochromosome 12p 13 , and some seminomas with isochromosome 12p retained activating KIT mutations, suggesting that the acquisition of isochromosome 12p precedes that of KIT mutations.…”

Section: Discussionmentioning

confidence: 74%

Comprehensive genetic analysis of pediatric germ cell tumors identifies potential drug targets

et al. 2020

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To elucidate the molecular pathogenesis of pediatric germ cell tumors (GCTs), we performed DNA methylation array analysis, whole transcriptome sequencing, targeted capture sequencing, and single-nucleotide polymorphism array analysis using 51 GCT samples (25 female, 26 male), including 6 germinomas, 2 embryonal carcinomas, 4 immature teratomas, 3 mature teratomas, 30 yolk sac tumors, and 6 mixed germ cell tumors. Among the 51 samples, 11 were from infants, 23 were from young children, and 17 were from those aged ≥10 years. Sixteen of the 51 samples developed in the extragonadal regions. Germinomas showed upregulation of pluripotent genes and global hypomethylation. Pluripotent genes were also highly expressed in embryonal carcinomas. These genes may play essential roles in embryonal carcinomas given that their binding sites are hypomethylated. Yolk sac tumors exhibited overexpression of endodermal genes, such as GATA6 and FOXA2, the binding sites of which were hypomethylated. Interestingly, infant yolk sac tumors had different DNA methylation patterns from those observed in older children. Teratomas had higher expression of ectodermal genes, suggesting a tridermal nature. Based on our results, we suggest that KIT, TNFRSF8, and ERBB4 may be suitable targets for the treatment of germinoma, embryonal carcinomas, and yolk sac tumors, respectively.

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Section: Discussionmentioning

confidence: 74%

Comprehensive genetic analysis of pediatric germ cell tumors identifies potential drug targets

et al. 2020

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“…Amongst germ cell tumours, DNA ploidy does not appear to be a prognostic factor in ovarian dysgerminomas, 74 whilst immature teratomas in a paediatric population are, irrespective of grade, diploid: 75 yolk sac tumours, by contrast, are aneuploid and combined immature teratomas/yolk sac tumours show a diploid teratomatous component and an aneuploid yolk sac component 75 …”

Section: Non‐epithelial Tumours Of the Ovarymentioning

confidence: 96%

Ploidy in gynaecological cancers

Fox

2005

Histopathology

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“…Five studies were based on analyses performed on 20 or more cases (135, 138–140, 142). The results from all 15 articles are summarized in Figure 2A and Supplemental Table 1.…”

Section: Genome Profiling Of Mogctmentioning

confidence: 99%

Molecular Characteristics of Malignant Ovarian Germ Cell Tumors and Comparison With Testicular Counterparts: Implications for Pathogenesis

et al. 2013

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This review focuses on the molecular characteristics and development of rare malignant ovarian germ cell tumors (mOGCTs). We provide an overview of the genomic aberrations assessed by ploidy, cytogenetic banding, and comparative genomic hybridization. We summarize and discuss the transcriptome profiles of mRNA and microRNA (miRNA), and biomarkers (DNA methylation, gene mutation, individual protein expression) for each mOGCT histological subtype. Parallels between the origin of mOGCT and their male counterpart testicular GCT (TGCT) are discussed from the perspective of germ cell development, endocrinological influences, and pathogenesis, as is the GCT origin in patients with disorders of sex development. Integrated molecular profiles of the 3 main histological subtypes, dysgerminoma (DG), yolk sac tumor (YST), and immature teratoma (IT), are presented. DGs show genomic aberrations comparable to TGCT. In contrast, the genome profiles of YST and IT are different both from each other and from DG/TGCT. Differences between DG and YST are underlined by their miRNA/mRNA expression patterns, suggesting preferential involvement of the WNT/β-catenin and TGF-β/bone morphogenetic protein signaling pathways among YSTs. Characteristic protein expression patterns are observed in DG, YST and IT. We propose that mOGCT develop through different developmental pathways, including one that is likely shared with TGCT and involves insufficient sexual differentiation of the germ cell niche. The molecular features of the mOGCTs underline their similarity to pluripotent precursor cells (primordial germ cells, PGCs) and other stem cells. This similarity combined with the process of ovary development, explain why mOGCTs present so early in life, and with greater histological complexity, than most somatic solid tumors.

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DNA Cytometry of Pure Dysgerminomas of the Ovary

Cited by 17 publications

References 0 publications

Comprehensive genetic analysis of pediatric germ cell tumors identifies potential drug targets

Comprehensive genetic analysis of pediatric germ cell tumors identifies potential drug targets

Ploidy in gynaecological cancers

Molecular Characteristics of Malignant Ovarian Germ Cell Tumors and Comparison With Testicular Counterparts: Implications for Pathogenesis

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