DNA damage and decrease of cellular oxidase activity in piglet sertoli cells exposed to gossypol

Abstract

The study was designated to explore the toxic effects of gossypol on piglet sertoli cells. Sertoli cells were isolated from piglet testes using a two-step enzyme digestion and followed by differential plating. Piglet sertoli cells were cultured and classified into five groups, that is, group A, the control without gossypol, group B with 2.5 μg/ml gossypol, group C with 5 μg/ml gossypol, group D with 10 μg/ml gossypol and group E with 20 μg/ml gossypol. We found that sertoli cells' growth was inhibited by gossypol at dose 2.5 μg/ml when compared with the control group. The oxidase activity of sertoli cell also decreased at 2.5 μg/m gossypol. Moreover, DNA damage of sertoli cells was observed at 5 μg/ml gossypol. Putting this into consideration, our study suggests that exposure of gossypol to sertoli cells leads to an inhibition of sertoli cell growth and oxidase activity of sertoli cells at a low concentration, whereas gossypol results in DNA damage of sertoli cells at a higher concentration.

The effects of feeding graded levels of whole cottonseed on semen characteristics and testicular profiles of Red Sokoto Bucks

“…Tanyildizi and Boskurt (2004) found that bulls fed cottonseed meal had significant increase in mid-piece sperm abnormalities appearing three weeks after the onset of the feeding trial. Ming et al (2011) also reported that gossypol treatment in piglets increased the percentage of dead spermatozoa. In contrast with the findings of Solaiman (2007) who reported that WCS as high as 30% in a buck diet may not cause any deleterious effect on sperm normality and progressive motility.…”

“…Significant decrease in sperm motility through an inhibitory effect of gossypol in WCS has been attributed to its effects on mitochondrial ATP production has been reported. (Ming et al, 2011;Timurkaan et al, 2011). It has also been reported that gossypol contained in WCS promotes formation of oxygen radicals, which may be the underlying basis of its biological activity (Braga et al, 2012).…”