DNA Damage and Repair in Higher Plants

Soyfer, Valery N.

doi:10.1016/b978-0-12-035408-5.50010-1

Cited by 17 publications

(8 citation statements)

References 129 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thesis, Odessa (in Russian)]. Later, using these data and having introduced caffeine exactly at the time of excision repair, we found that caffeine enhanced the frequency of chromosomal aberrations several fold at the time of maximal enzyme repair activity (Soyfer 1979).…”

Section: Introductionmentioning

confidence: 81%

See 1 more Smart Citation

Influence of physiological conditions on DNA repair and mutagenesis in higher plants

Soyfer¹

1983

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

In barley (Hordeum vulgare L.) and grass pea (Lathyrus sativus L.), caffeine, an inhibitor of DNA repair activity, and Na2ethylenediaminetetraacetate, an inhibitor of DNA‐endonucleases, sharply decreased the excision repair of pyrimidine dimers induced in DNA by ultraviolet irradiation. An inhibitor of RNases, diethylpyro‐carbonate, did not inhibit the process of excision, and in one experiment it even enhanced excision. Caffeine markedly increased the frequency of mutations and inhibited the growth of seedlings after UV‐radiation. Such enhancement was greater with the higher UV fluence. Results of chemical inhibition were further confirmed by the suppression of repair by low temperatures: the frequency of chromatid aberrations induced with propyl methanesulfonate was increased more than 3 times and chromatid aberrations 1.5 times. Evidence for participation of repair enzymes in the modification of mutation processes was also obtained in the experiments which combined γ‐irradiation and treatment with propyl methanesulfonate. Conditions favouring repair activity caused a drastic reduction in the frequency of aberrations, whereas with conditions preventing enzyme function the mutation frequency increased. In one of the experiments of this series we were able to demonstrate, with identical mutagenic treatment, that by changing post‐mutagen conditions (wetting and drying of seeds, storage after mutagenic treatment) it was possible to alter the mutation frequency and to obtain below‐additive, additive and synergistic mutational response.

show abstract

Section: Introductionmentioning

confidence: 81%

“…After 30 min washing, the seeds were dried with a hand-held hair dryer at a controlled temperature (18°C) to constant weight and put into a freezing chamber at -70°C for 24 h. DNA isolation. DNA was isolated according to the method described earlier (Soyfer andCieminis 1974, 1977).…”

Section: Influence Of Inhibitorsmentioning

confidence: 99%

Influence of physiological conditions on DNA repair and mutagenesis in higher plants

Soyfer¹

1983

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ineffective "polymorphism" (used in a wider sense, including functional polymorphism involving a set of genes as well as single gene polymorphism) or deficiency, in any one of the genetic systems responsible for the enzymes in the pathway may stop or seriously hamper the processes of repair. Although DNA repair systems have been found in prokaryotes and have also been shown to occur in certain animal and plant cells, it is evident from the review of literature (Wolff and Cleaver, 1973;Yamaguchi et aL, 1975;Kihlman, 1977;Tano and Yamaguchi, 1977;Soyfer, 1979;Jackson and Linskens, 1980;Johnson et aL, 1984) that the belief, by some authors, of their relatively uniform, universal occurrence, with regard to tissues as well as species, in plants may not be justified. The notion that DNA repair as a process is common to all life, and evolved very early, is generally accepted.…”

Section: Hypothesismentioning

confidence: 98%

Gene transfer through the use of sublethally irradiated pollen: the theory of chromosome repair and possible implication of DNA repair enzymes

Pandey

1986

Heredity

View full text Add to dashboard Cite

Gene transfer experiments using sublethally irradiated pollen have given inconsistent results, with the species used falling generally into two classes: (a), those in which the Ml plants, arising from the use of irradiated pollen, are highly variable and show a "maternalisation effect", many plants lacking one or more dominant markers from the irradiated parent and (b), those in which the Ml plants are generally alike and similar to the Fl plants obtained by the use of unirradiated pollen, and there is no maternalisation effect. It is suggested here that this difference relates to the absence (a) or presence (b) of certain chromosome and DNA repair enzymes in the pollen, for which, it is argued, plant species may be widely variant and "polymorphic". The proposal is in agreement with the theory of chromosome repair through somatic recombination and gene conversion previously invoked to explain the maternalisation effect and gene transfer through the use of sublethally irradiated pollen.

show abstract

“…This problem, unstudied for a long time, is now being investigated in several laboratories [19,20]. It is already well established that higher plants are able to repair demage caused bl ultraviolet, ionizing radiations or chemicals.…”

Section: Discussionmentioning

confidence: 99%

Base‐Excision Repair in Carrot Cells

Talpaert-Borlé

Liuzzi

1982

European Journal of Biochemistry

View full text Add to dashboard Cite

Uracil-DNA glycosylase and apurinic/apyrimidinic (AP) endodeoxyribonuclease hacc been purified from cultured carrot cells.The two enzymes, separated by affinity chromatography on Sepharosc-pc,ly(rU), were found to have properties similar to those of the homologous bacterial and mammalian enzymes. 1 he action of AP endodeoxyribonuclease on (dA)230 . (dT, dU)230 partially depyrimidinated by uracil-DNA 811 co\ylase suggests that these two enzymes might act successively to initiate the repair of uracil-containing DNA Inforination concerning enzymes implicated in the DNA repair of plant cells is scarce. Nevertheless, the available data suggest that the repair mechanisms are similar to those described in bacterial and in mammalian cells. For instance, endodeoxyribonucleases for apurinic sites were studied in tissues of Phascolus mult(f1orus [I, 21 and barley [3,4].The apuriniciapyrimidinic (AP) endodeoxyribonuclease acts in an early step of the excision repair pathway by cutting the DNA backbone near a missing base lost either spontaneously or as the result of the action of specific DNA glycosylases. These latter enzymes, recently found in bacterial and in mammalian cells, catalyze the hydrolysis of the glycosyl bond between the deoxyribose moiety and an altered or incorrect base in DNA [5]. In this new class of enzymatic activities, uracil-DNA glycosylase is involved in the removal of uracil erroneously incorporated in newly synthesized D N A or produced by deamination of cytosine.This paper presents some data on the isolation and characterization of an uracil-DNA glycosylase from cultured carrot cells. An AP endodeoxyribonuclease which subsequently acts in the repair of DNA was also detected and partially characterized. The present findings suggest the existence of a base-excision repair mechanism in plant cells. up to 1 ml with water, supplemented with 10 ml of Insta-Gel (Packard) and counted in a liquid scintillation spectrometer. The results were corrected by subtracting the radioactivity of controls which were prepared under the same conditions without enzyme, and which gave ;I very low acid-soluble fraction. We adopted the unit of AI' endodeoxyribonuclease defined by Verly and Rassart [S] as the amount which releases 10 :4 of the total alkylated-depurinatcd DNA radioactivity into the acid-soluble fraction in 1 he conditions described above. MATERIALS A N D METHODS Subs t ra tosAssuy .for Uracil-DNA Glycos~~lns~~. The enzyme activity was measured as outlined previously [7]. The standard reaction mixture (50 pl) contained 10 tnM TrisjHCI, pH 7.8,

show abstract

DNA Damage and Repair in Higher Plants

Cited by 17 publications

References 129 publications

Influence of physiological conditions on DNA repair and mutagenesis in higher plants

Influence of physiological conditions on DNA repair and mutagenesis in higher plants

Gene transfer through the use of sublethally irradiated pollen: the theory of chromosome repair and possible implication of DNA repair enzymes

Base‐Excision Repair in Carrot Cells

Contact Info

Product

Resources

About