DNA damage and somatic mutations in mammalian cells after irradiation with a nail polish dryer

Abstract

Ultraviolet A light is commonly emitted by UV-nail polish dryers with recent reports suggesting that long-term use may increase the risk for developing skin cancer. However, no experimental evaluation has been conducted to reveal the effect of radiation emitted by UV-nail polish dryers on mammalian cells. Here, we show that irradiation by a UV-nail polish dryer causes high levels of reactive oxygen species, consistent with 8-oxo-7,8-dihydroguanine damage and mitochondrial dysfunction. Analysis of somatic mutations reveals a dose-dependent increase of C:G>A:T substitutions in irradiated samples with mutagenic patterns similar to mutational signatures previously attributed to reactive oxygen species. In summary, this study demonstrates that radiation emitted by UV-nail polish dryers can both damage DNA and permanently engrave mutations on the genomes of primary mouse embryonic fibroblasts, human foreskin fibroblasts, and human epidermal keratinocytes.

Polyphenolic Platform Ameliorated Sanshool for Skin Photoprotection

“…( Figure 5 A ) Thus, γH2AX immunofluorescence was employed to evaluate the protective mechanism of PS NPs on skin tissues. [ 43 ] The occurrence of DNA double‐strand breaks (DSBs) is widely recognized as a perilous issue that can have severe repercussions on cell viability. [ 44 ] These breaks are typically a result of the interaction between cellular DNA and ROS that are activated by UV.…”

Influence of UV nail lamps radiation on human keratinocytes viability

“…The study was investigated in response to a recently published article by Maria Zhivagui et al, according to which there is strong evidence suggesting that the radiation emitted by UV nail dryers may cause cancers of the hand and that UV-nail polish dryers, similar to tanning beds, may increase the risk of early-onset skin cancer 7 . In our study, the human keratinocyte cell line HaCaT was exposed to UV for 4 min, which corresponded to the real conditions of typical exposure time per manicure being around 5 min per hand by UV nail polish dryers 10 – 12 .…”

“…Products cured in UV lamps became popular in the early 1990s 3 , 6 . They change their state of aggregation thanks to the presence of photoinitiators that absorb the UVA lightproduced by manicure lamps and dissolve, releasing free radicals that initiate the polymerization process 7 . In order for the reaction to be complete and correct, the duration of irradiation and the lamp power should be adjusted to the manufacturer's recommendations and the formulation of a given product.…”

Recent Progress in Polymers with Dynamic Covalent Bonds

“…Even though the involvement of dimerization yields highly functional materials, all the studies mentioned above required UV irradiation, which is harmful to the human body. [ 91,92 ] Therefore, halochromic chromophore like styrylquinoxaline was employed to induce a red light‐induced photocycloaddition. [ 72,93 ] Recently, a hydrogel that responded to different visible light was developed based on two red‐shifted chromophores, styrylpyrido[2,3‐b]pyrazine (SPP) and acrylpyerene (AP) as depicted in Figure .…”

“…Even though the involvement of dimerization yields highly functional materials, all the studies mentioned above required UV irradiation, which is harmful to the human body. [91,92] Therefore, halochromic chromophore like styrylquinoxaline was [86] Copyright 2018, The Authors, published by American Association for the Advancement of Science.…”