DNA damage in hemocytes of Schistocerca gregaria (Orthoptera: Acrididae) exposed to contaminated food with cadmium and lead

Yousef, Hesham A.; Afify, A. M.; Hasan, Hany M.; Meguid, Afaf Abdel

doi:10.4236/ns.2010.24037

Cited by 16 publications

(15 citation statements)

References 24 publications

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“…These results may be due to DNA repair occurred which differ from the study of Yousef et al (2010) that there were a higher DNA damage in the mature adults with respect to the long period of feeding of Shistocerca gregaria on clover treated with CdCl 2 and PbCl 2 reflects the absence of repair mechanism. But there are many studies used the comet assay to observe DNA repair in irradiated cells (Isabel and Maria, 2014) and in different cells of Drosophila after exposure to different genotoxic agents (Bilbao et al, 2002;Mukhopadhyay et al, 2004;Siddique et al, 2005bSiddique et al, , 2013García -Sar et al, 2012;Sharma et al, 2012;Mishra et al, 2014;Gaivã o et al, 2014;Isabel and Maria, 2014).…”

Section: Genotoxicity and Dna Repaircontrasting

confidence: 54%

“…Some studies used small mammalian species, living in or close to polluted areas for detection of hazardous pollution (Farbairn et al, 1995;Petras et al, 1995;Tice, 1995;Baker et al, 1996;Salagovic et al, 1996;Ralph et al, 1997;Silva et al, 2000). Also, comet assay has been applied to cells of insects, including Shistocerca gregaria, Drosophila melanogaster, Curculio sikkimensis, mosquito larvae, Ephestia kuehniella and in grasshoppers Chorthippus brunneus (Siddique et al, 2005;Todoriki et al, 2006;Augustyniak et al, 2006;Yousef et al;, Abdel-Gawad et al, 2011Isabel and Maria, 2014;Pandir and Guven, 2014).…”

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confidence: 99%

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Potential Role of Mosquito Larvae <i>Culex Pipiens</i> as a Biological Indicator of Environmental Water Pollution in Egypt

Afify

2017

jmr

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River Nile represents the most important source of freshwater in Egypt. There are several factors lead to the water pollution in the River Nile System (main stream Nile, drains and canals). The water quality of River Nile was good despite high organic loads discharged from some of the drains and industrial activities. These were harmful both to human and stream ecosystem health. A biological approach to monitoring water quality incorporates use of stream organisms themselves as a basis for pollution detection. Fish and aquatic insects considered as bio-indicators of pollutant effects and help to investigate possible environmental problems. One of the recent biochemical techniques for detecting DNA damage as a result of DNA single strand breaks, alkali-labile sites, and cross-linking is the alkaline (pH>13) single cell gel SCG assay. In this study the comet assay measure the DNA damage in various stages of the mosquito Culex pipiens collected from two different polluted water streams (Nikla and Elmansoreyh). The DNA damage measured in 3 rd , 4 th larval instars, pupa, male and female adults. It is clear from the results obtained in this study that the genotoxicity of water pollution of two different polluted water streams (Nikla and Elmansoreyh) in C. pipiens was high in larval and pupal stages and this study affirmed the appropriateness of the comet assay as a sensitive tool for environmental monitoring. Additionally, it can be proposed that C. pipiens is a strong aquatic bioindicator of genotoxicity.

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Section: Genotoxicity and Dna Repaircontrasting

confidence: 54%

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confidence: 99%

Potential Role of Mosquito Larvae <i>Culex Pipiens</i> as a Biological Indicator of Environmental Water Pollution in Egypt

Afify

2017

jmr

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“…The DNA damage and repair were quantified by the comet assay (alkaline single cell gel electrophoresis) as described by Yousef et al . () and Yun et al . () with slight modifications.…”

Section: Methodsmentioning

confidence: 89%

Evaluation of genotoxic and cytotoxic effects of ethyl acetate extract of Aspergillus flavus on Spodoptera litura

et al. 2019

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“…It is known that the genotoxicity of cadmium may involve either indirect or direct interaction with DNA and produce cytogenetic damage, as a consequence of DNA strand breaks (Waalkes, Misra 1996), which we observed in all studied benthic chironomid species. Also, Pb ions induced either single or double DNA breaks or inhibited the DNA replication or repair process (Yousef et al 2010) influencing the calcium regulated process. Because we detected a high spectrum of somatic chromosome alterations in all these species, it could be assumed that these alterations were induced by high concentrations of the above-mentioned metals.…”

Section: Discussionmentioning

confidence: 99%

The effect of long-term contamination by heavy metals on community and genome alterations of Chironomidae (Diptera) in a stream with mine drainage water (southern Poland)

Szarek-Gwiazda

Michailova

Ilkova

et al. 2013

Oceanological and Hydrobiological Studies

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This paper reports on studies of the effect of heavy metals on the Chironomidae that inhabit the Matylda stream, which has been contaminated for about 100 years by discharge water from a zinc and lead ore mine. Stream sediment was strongly polluted by Cd, Pb, Cu and Zn. These metals did not affect the Chironomidae community but strongly changed the genome system of the Chironomidae species that inhabited the sediment. The salivary gland chromosomes of six species belonging to the two genera Chironomus and Prodiamesa are analyzed. In all species the somatic index is calculated on the basis of somatic * Corresponding author: szarek@iop.krakow.pl chromosome alterations. Chironomus riparius has the highest numbers of somatic alterations and the highest somatic index -9.67. The smallest chromosome G carries the key structures known as "Balbiani rings", which play an important role in species development. This chromosome is very sensitive in the genome of the most studied species. The high sensitivity of the C. riparius genome is discussed in light of its DNA organization. The results show a high response of the salivary gland chromosomes to heavy metal pollution, and this makes them a valuable indicator in the assessment of water quality and detection of mutagenic agents in the aquatic environment.

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DNA damage in hemocytes of Schistocerca gregaria (Orthoptera: Acrididae) exposed to contaminated food with cadmium and lead

Cited by 16 publications

References 24 publications

Potential Role of Mosquito Larvae <i>Culex Pipiens</i> as a Biological Indicator of Environmental Water Pollution in Egypt

Potential Role of Mosquito Larvae <i>Culex Pipiens</i> as a Biological Indicator of Environmental Water Pollution in Egypt

Evaluation of genotoxic and cytotoxic effects of ethyl acetate extract of Aspergillus flavus on Spodoptera litura

The effect of long-term contamination by heavy metals on community and genome alterations of Chironomidae (Diptera) in a stream with mine drainage water (southern Poland)

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