DNA damage in multiple organs after exposure to chlorhexidine in Wistar rats

Grassi, Thiago Luís Magnani; Camargo, Elaine Aparecida de; Salvadori, Daisy Maria Fávero; Marques, Sílvio Alencar; Ribeiro, Daniel Araki

doi:10.1016/j.ijheh.2006.09.001

Cited by 41 publications

(42 citation statements)

References 27 publications

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“…Hermeto et al 2014;Timoroglu et al, 2014). In circulating blood samples (Grassi et al, 2007;Braz et al, 2011;Costa et al, 2011;Kasuba et al, 2012), for example, it is not common to find damage frequently, which may also occur due to clonal expansion, when the pH > 13 comet assay version is used.…”

Section: Discussionmentioning

confidence: 99%

“…Studies in which an automatic analysis system is used to determine DNA damage using the comet assay show limited parameters such as only the DNA percentage in the tail (Osipov et al, 2014) or the tail intensity (Priestley et al, 2010;Braz et al, 2011;Costa et al, 2011;Camargo et al, 2013;Valença-Silva et al, 2014), or this parameter is associated with others, such as tail moment and tail length, in the results (Grassi et al, 2007;Kasuba et al, 2012;Aydin et al, 2013;Pant et al, 2014;Timoroglu et al, 2014). However, there is still no consensus regarding the best parameter for MSCs or other biological samples.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay

et al. 2015

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ABSTRACT. The use of mesenchymal stem cells (MSCs) in experimental, clinical, and therapeutic trials has grown in recent years. However, the issue remains of whether these procedures are completely safe for transplant patients. Therefore, this study was designed and carried out with the aim of evaluating two different comet assay protocols for genomic damage pattern analysis in MSCs derived from adipose tissue. The analyzed and interpreted results suggest that genetic testing is needed to support clonal expansion safety in cell therapy procedures with MSCs. Furthermore, they also suggest that if the comet assay technique would be used as a genomic integrity screening assay, the protocol performed at pH = 12 (that yielded a frequency of damaged cells: tail intensity = 9.50 ± 0.60, tail moment = 0.0122 ± 0.0007; results are reported as means ± standard deviation) would be indicated as genomic damage, and that subsequent single-strand breaks occur at pH > 13 (frequency of damaged cells: tail intensity = 30.71 ± 4.23, tail moment = 0.0447 ± 0.0073). Our study demonstrates that, in the era of regenerative medicine, it is necessary to standardize and establish a battery of tests in order to identify genomic damage prior to MSC transplantation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay

et al. 2015

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“…According to Nancy and Don [32], cultured human periodontal cells treated with 2% chlorhexidine exhibited a foamy appearance in which most of the cytoplasm seemed to have been extracted from the cells. According to an in vivo genotoxicity study conducted by Grassi et al [33] in rats, chlorhexidine induced DNA damage in leukocytes, renal cells and oral mucosal cells. And in an in vitro study on rat oral mucosal cells and rat leukocytes, Ribeiro et al.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Assessment of the Toxic Effects of an AKWATON based-disinfectant on Human Tissues

MK¹,

Lesage²,

Gauvin³

et al. 2017

J Antimicrob Agents

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The purpose of this study is to prove the potential safe use of AKWATON as a new antimicrobial product. Many service products are often removed from the market due to their toxic effects on the human body or to their aggressiveness towards the environment. Antimicrobial products such as disinfectants may contain harmful ingredients that can cause disease. Some disinfecting products are corrosive or irritating; others produce strong odors, which in the long run can cause real health problems. AKWATON is a new disinfectant, member of the family of guanidine polymers.Its bactericidal, fungicidal and sporicidal properties have been demonstrated and widely documented. In this study, the toxic effects of AKWATON and of three well known commercial antimicrobial products currently on market, were evaluated and compared on various human tissues including eyes, lung, skin and liver cells. The testing were performed using the TB (Trypan blue) and MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) methods. Cell-cultures and the different tests done, showed that the AKWATON based-disinfectant was much less toxic, killing many fewer cells than the commercial disinfectants. It spared more than 64% of skin cells; 65% of lung cells; 66% of eye cells and 64% of liver cells while some well-known disinfectants currently marketed killed 100% of cells. This study demonstrated that AKWATON can be used as an odorless, colorless, non-corrosive and harmless disinfectant for hospital, agriculture industry, farming, food service and household facilities or as antiseptic.

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“…Others have demonstrated that chlorhexidine does not induce genetic damage in vitro (57,65). Nevertheless, chlorhexidine is potent at causing genotoxic damage in several tissues and organs, such as liver, kidney and oral mucosa cells in vivo (66)(67)(68)(69). When associated with calcium hydroxide, chlorhexidine exerts both anti-oxidant and prooxidant activities through scavenging superoxide radicals by the xanthine/xanthine oxidase reaction and induction of reactive species production including hydrogen peroxide and superoxide radicals, respectively (70).…”

Section: Endodontic Compoundsmentioning

confidence: 99%

Genotoxicity Induced by Dental Materials: A Comprehensive Review

2017

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DNA damage in multiple organs after exposure to chlorhexidine in Wistar rats

Cited by 41 publications

References 27 publications

Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay

Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay

In Vitro Assessment of the Toxic Effects of an AKWATON based-disinfectant on Human Tissues

Genotoxicity Induced by Dental Materials: A Comprehensive Review

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