2006
DOI: 10.1016/s0076-6879(05)09010-5
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DNA Damage‐Induced Phosphorylation of Rad55 Protein as a Sentinel for DNA Damage Checkpoint Activation in S. cerevisiae

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Cited by 14 publications
(20 citation statements)
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“…In another example, which supports a key role for Mec1 in the control of HR, phosphorylation of multiple S/T‐Q residues in Sae2, a pro‐resection factor homologous to human CtIP, contributes to Mec1‐mediated GCR suppression (Liang et al , ). Furthermore, Mec1 phosphorylates the strand exchange factor Rad55, and Rad53 promotes the phosphorylation and DNA binding of the Tid1/Rdh54 translocase (Fig ; Bashkirov et al , ; Ferrari et al , ; Herzberg et al , ). Mec1 also phosphorylates Rad51 at serine 192, likely supporting its ATPase activity and capacity for strand invasion (Fig ; Flott et al , ).…”
Section: Introductionmentioning
confidence: 99%
“…In another example, which supports a key role for Mec1 in the control of HR, phosphorylation of multiple S/T‐Q residues in Sae2, a pro‐resection factor homologous to human CtIP, contributes to Mec1‐mediated GCR suppression (Liang et al , ). Furthermore, Mec1 phosphorylates the strand exchange factor Rad55, and Rad53 promotes the phosphorylation and DNA binding of the Tid1/Rdh54 translocase (Fig ; Bashkirov et al , ; Ferrari et al , ; Herzberg et al , ). Mec1 also phosphorylates Rad51 at serine 192, likely supporting its ATPase activity and capacity for strand invasion (Fig ; Flott et al , ).…”
Section: Introductionmentioning
confidence: 99%
“…Evidence from budding yeast implicates DNA damage response kinases in regulating DNA repair, as cells lacking MEC1 are defective in homologous recombination (Fasullo & Sun, 2008; Suetomi et al , 2010). Although the underlying mechanisms for this are not clear, Mec1‐ and Tel1‐dependent phosphorylation of the Sae2 protein has been shown to promote DSB resection (Baroni et al , 2004), and homologous recombination is further promoted by Mec1‐dependent phosphorylation of Rad55 (Bashkirov et al , 2006; Herzberg et al , 2006). Here, we establish that Rad51 is phosphorylated on Ser 192 in response to DNA damage in a Mec1‐dependent manner, and that mutation of this site confers hypersensitivity to DNA damage and defective homologous‐recombination‐mediated repair of a homothallic switching (HO) endonuclease‐induced DSB.…”
Section: Introductionmentioning
confidence: 99%
“…Rad55-Rad57 (wild type and Rad55-S2,8,14A-Rad57) was purified as glutathione Stransferase-Rad55 and His 6 -Rad57 fusions after the overexpression from the GAL1-10 promoter of a pJN58 derivative. To obtain the damage-induced phosphorylated form of the heterodimer, 0.1% MMS was added for 2 h. About 90 g of cells was used for protein purification using sequential affinity chromatography on glutathione-Sepharose and Ni-nitrilotriacetic acid agarose resins as previously described (3). The kinase assay with purified Rad53 kinase and the Rad55-Rad57 heterodimer (1 g) was performed as previously described (2), followed by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Coomassie-staining, and imaging.…”
Section: Methodsmentioning
confidence: 99%