DNA damage response pathway in radioadaptive response

Sasaki, Masao S.; Ejima, Yoshimichi; Tachibana, Akira; Yamada, Toshiko; Ishizaki, Kanji; Shimizu, Takashi; Nomura, Taisei

doi:10.1016/s0027-5107(02)00084-2

Cited by 155 publications

(87 citation statements)

References 122 publications

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“…This cytoprotective response was optimally induced when the cells were irradiated with γ-rays at 0.25-0.5 Gy. In contrast, it was reported that the DNA/chromosomal protection was optimally induced when various mammalian cell types were pre-irradiated below ~0.1 Gy (Ikushima et al, 1996;Shimizu et al, 1999;Sasaki et al, 2002). Therefore, the optimal dose range for IR-induced cytoprotective responses appears to vary depending on experimental settings.…”

Section: Discussionmentioning

confidence: 95%

“…Some investigators explored this latter possibility by using the TUNEL assay, a method widely used to diagnose DNA fragmentation induced during apoptosis (McGahon et al, 1995). The reported results suggest that the pre-irradiation can reduce the challenge-induced accumulation of TUNEL-positive cells (Takahashi et al, 2001;Sasaki et al, 2002). While this data was interpreted in a way that the IR-induced cytoprotection can act against apoptosis, it should be noted that the TUNEL assay reflects genotoxic effects of IR, not necessarily apoptosis.…”

Section: Ionizing Radiation Induces Blockade Of C-jun N-terminal Kinamentioning

confidence: 99%

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Ionizing radiation induces blockade of c-Jun N-terminal kinase-dependent cell death pathway in a manner correlated with p21Cip/WAF1 induction in primary cultured normal human fibroblasts

et al. 2005

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During radiotherapy of cancer, neighboring normal cells may receive sub-lethal doses of radiation. To investigate whether such low levels of radiation modulate normal cell responses to death stimuli, primary cultured human fibroblasts were exposed to various doses of γ-rays. Analysis of cell viability using an exclusion dye propidium iodide revealed that the irradiation up to 10 Gy killed the fibroblasts only to a minimal extent. In contrast, the cells efficiently lost their viability when exposed to 0.5-0.65 mM H2O2. This type of cell death was accompanied by JNK activation, and was reversed by the use of a JNK-specific inhibitor SP600125. Interestingly, H 2 O 2 failed to kill the fibroblasts when these cells were pre-irradiated, 24 h before H 2 O 2 treatment, with 0.25-0.5 Gy of γ-rays. These cytoprotective doses of γ-rays did not enhance cellular capacity to degrade H2O2, but elevated cellular levels of p21 Cip/WAF1 , a p53 target that can suppress H 2 O 2 -induced cell death by blocking JNK activation. Consistently, H 2 O 2 -induced JNK activation was dramatically suppressed in the pre-irradiated cells. The overall data suggests that ionizing radiation can impart normal fibroblasts with a survival advantage against oxidative stress by blocking the process leading to JNK activation.

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Section: Discussionmentioning

confidence: 95%

Section: Ionizing Radiation Induces Blockade Of C-jun N-terminal Kinamentioning

confidence: 99%

Ionizing radiation induces blockade of c-Jun N-terminal kinase-dependent cell death pathway in a manner correlated with p21Cip/WAF1 induction in primary cultured normal human fibroblasts

et al. 2005

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“…However, in mp53 cells, induction of the radio-adaptive response was not observed. p53, which plays a key role in protecting the genome (Lane, 1992), is the most important factor in the signaling pathway of the radioadaptive response because various endpoints used to study this response such as changes in apoptosis levels (Wang et al, 2000;Hendrikse et al, 2000;Takahashi, 2001;Okazaki et al, 2007), micronuclei induction (Sasaki et al, 2002) and chromosome aberrations (Takahashi et al, 2008b) are not observed in p53-null and mp53 cells. It should be noted that an accumulation of p53 was induced after a high-dose irradiation alone, but was not induced after a priming irradiation followed by a subsequent challenging irradiation in wtp53 cells (Takahashi et al, 2008b).…”

Section: − 29 −mentioning

confidence: 99%

The First Life Science Experiments in ISS: Reports of "Rad Gene"-Space Radiation Effects on Human Cultured Cells-

Takahashi

Nagamatsu

et al. 2010

Biol. Sci. Space

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To clarif y the biological ef fe cts of space environment, especially space radiations, a proposal of " Rad Gene" was performed as the first life science experiment with two human lymphoblastoid cell lines bearing wild-type p53 gene (wtp53) and mutated p53 gene (mp53) in an International Space Station (ISS) for 133 days. We scheduled four projects: (1) DNA damage induced by space radiations including the high linear energy transfer (LET) particles was detected as a track of γH2AX foci in the nuclei of these frozen cells. (2) To examine the biological effects of microgravity and space radiations on gene and protein expression of p53 -dependent regulated genes, these cells were grown under microgravity and 1 gravity in ISS, and on ground for 8 days and analyzed by DNA and protein arrays. (3) p53-Dependent regulated genes were analyzed in the cultured cells after spaceflight at frozen state exposed to space radiations. (4) To clarify the effects of space radiations on the radioadaptive response, the space flown cells at frozen state were cultured, and then exposed to challenging X-ray-irradiation. All of the radioadaptive responses of cell killing, apoptosis, chromosomal aberrations and mutations were found only in wtp53 cells, but not in the mp53 cells.

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“…A number of studies and reviews (Bala & Mathew, 2000;Luckey, 2008;Pandey et al, 2006;Sasaki et al, 2002) document that exposure to small doses of ionizing radiation enhanced the tolerance towards the detrimental effects of lethal doses of ionizing radiation given subsequently. Such phenomenon was observed in prokaryotes as well as in eukaryotes.…”

Section: Introductionmentioning

confidence: 99%

Radiation Induced Radioresistance - Role of DNA Repair and Mitochondria

Bala¹

2012

Gamma Radiation

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DNA damage response pathway in radioadaptive response

Cited by 155 publications

References 122 publications

Ionizing radiation induces blockade of c-Jun N-terminal kinase-dependent cell death pathway in a manner correlated with p21Cip/WAF1 induction in primary cultured normal human fibroblasts

Ionizing radiation induces blockade of c-Jun N-terminal kinase-dependent cell death pathway in a manner correlated with p21Cip/WAF1 induction in primary cultured normal human fibroblasts

The First Life Science Experiments in ISS: Reports of "Rad Gene"-Space Radiation Effects on Human Cultured Cells-

Radiation Induced Radioresistance - Role of DNA Repair and Mitochondria

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