DNA damaging potential of zinc oxide nanoparticles in human epidermal cells

Sharma, Vyom; Shukla, Ritesh K.; Saxena, Neetu; Parmar, Devendra; Das, Mukul; Dhawan, Alok

doi:10.1016/j.toxlet.2009.01.008

Cited by 566 publications

(339 citation statements)

References 45 publications

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“…In wild-type, the presence of metalloproteins provides antioxidant protection to the organism; this protection is significantly impaired in the triple knockout mutant, findings that mirror those of others (Sharma et al, 2012a,b;Ahamed et al, 2011;Sharma et al, 2009;Hanley et al, 2009;Huang et al, 2010).…”

Section: Resultssupporting

confidence: 73%

“…However, the exposure to ZnONPs resulted in a significant (p≤0.05) decrease in the total intracellular ROS levels in the wild-type strain, as the total fluorescence intensity of the DCF moiety decreased by 22% compared to the control levels. In , as hypothesized in studies that highlighted the carcinogenicity of ZnONPs (Sharma et al, 2012a,b;Ahamed et al, 2011;Sharma et al, 2009;Wu et al, 2010).…”

Section: Resultsmentioning

confidence: 84%

“…Exposure of the cellular environment to ZnONPs can oxidize and reduce macromolecules (proteins, lipids, DNA), thereby causing oxidative damage to the cell (Sharma et al, 2012a,b;Sharma et al, 2009). In addition, due to their size, some ZnONPs may reach the nucleus and interact with DNA molecules (Sharma et al, 2012a,b;Martinez et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

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Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Polak

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Jurkschat

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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Spurgeon, David J.; Sturzenbaum, Stephen R.. 2014. Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegansContact CEH NORA team at noraceh@ceh.ac.ukThe NERC and CEH trademarks and logos ('the Trademarks') are registered trademarks of NERC in the UK and other countries, and may not be used without the prior written consent of the Trademark owner. suggesting that the nominal exposure to pure ZnONPs represents in vivo, at best, a mixture exposure of ionic zinc and nanoparticles. Nevertheless, the analyzes provided evidence that the metallothioneins (mtl-1 and mtl-2), the pytochelatin synthase (pcs-1) and an apoptotic marker (cep-1) were transcriptionally activated. In addition, the DCFH-DA assay provided in vitro evidence of the oxidative potential of ZnONPs in the metal exposure sensitive triple mutant.Raman spectroscopy highlighted that the biomolecular phenotype changes significantly in the metal sensitive knockout worm upon ZnONP exposure, suggesting that these metalloproteins are instrumental in the protection against cytotoxic damage. Finally, ZnONP exposure was shown to decrease growth and development, reproductive capacity and lifespan, effects which were amplified in the triple knockout. By combining diverse toxicological strategies, we identified that individuals (genotypes) housing mutations in key metalloproteins are more susceptible to ZnONP exposure, which underlines the importance of fully functional metalloproteins to minimize ZnONP induced toxicity.

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Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 84%

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Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Polak

Read

Jurkschat

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Silver nanoparticles (AgNPs) widely used in antimicrobial activity, cosmetics industry and daily products. Synthesis method of nanoparticles plays an important role in its technological advancement as it is the primary step to tune their physical, electronic, and optical properties of the synthesized nanoparticles by varying size, shape and surface chemistry (Sharma et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Leaf Extract of Rosmarinus officinalis and Its Effect on Tomato and Wheat Plants

Farghaly¹,

Nafady²

2015

JAS

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Biological green synthesis of silver nanoparticles (AgNPs) from silver salts is a growing advanced approach to avoid the requirement of costly instruments and involvement of hazardous chemicals as well. However, increasing use of AgNPs raises potential toxicity level in the environment. In this investigation, leaf extract of rosemary (Rosmarinus officinalis) used as a reducing and stabilizing agent for biosynthesis of AgNPs. The biosynthesized AgNPs were authorized by UV-vis spectrophotometer and X-ray diffraction (XRD) analysis. The shape and size of the biosynthesized AgNPs were studied using high resolution transmission electron microscope (TEM). The toxicity of the biosynthesized silver nanoparticles on wheat and tomato plants was studied by soaking wheat grains and tomato seeds in 100 mg/L AgNPs and follow its effect on seedling growth of wheat (at 10 days) and on vegetative growth of tomato and wheat plants (at 35 days). Some physiological parameters as germination percentage of wheat seedling, length of seedling, dry weight, pigment fractions (chl.a, chl.b and caroteinoids), soluble proteins, lipid peroxidation (MDA) and antioxidant enzymes (catalase and peroxidase) of two plants were measured. AgNPs has a non-significant inhibitory effect on germination percentage of wheat, dry weight and pigment fractions. The biosynthesized AgNPs has a noticeable stress effect on tomato plant as reduced chlorophyll a and dry weight. Generally, AgNPs stimulate MDA accumulation in tomato and wheat plants. There was a noticeable different effect of AgNPs on soluble proteins and antioxidant enzymes as catalase and peroxidase among tomato and wheat plants.

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“…Zinc in low doses has benefi cial functions in metalloenzymes, transcription factors, immune regulation and growth and synthesis of cell proteins and plays antioxidant, anti-apoptotic and anti-infl ammation roles, but in high doses, it has toxic effects and causes oxidative stress through increasing end products of lipid peroxidation and decreasing glutathione and protein thiol levels (23). Cytotoxicity increases with rise in nanoparticles concentration and also exposure time (24). The nanoparticles of zinc oxide can pass from protective layers of body; enter the circulation and lead to severe effects (25) especially predispose individuals to thrombosis in high doses (26).…”

Section: Introductionmentioning

confidence: 99%

Toxic effect of nano-zinc oxide

Hr¹,

Faghfoori²,

Izadpanah³

et al. 2015

BLL

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INTRODUCTION: Nowadays, a lot of studies were carried out about side effects of nanoparticles especially nano-zinc oxide, but unfortunately, most of these studies have been conducted with high doses. MATERIAL AND METHODS: In the present study 36 male Wistar rats with approximate age of two months and weighing 300 ± 30 g were selected. After standard phases of preparation, injections were done intraperitoneally in chronic groups with zinc oxide nanoparticles (20 and 40 mg/kg) every other day and for acute groups with 200 and 400 mg/kg in a single dose. After the determined time for every group (21 days for chronic groups and 7 days for acute groups), blood samples were provided from rat hearts and also liver, kidney and testicular tissue were removed for histological studies. RESULTS: There were signifi cant decreases in lymphocytes and platelet counts and signifi cant increases in white blood cells count, hemoglobin and hematocrit in acute dose compared to control group and no difference in red blood cells count in all groups. A severe to slight damage was observed in liver tissue in all groups and tissue destruction was seen in kidney only in acute dose treated groups but no specifi c pathological effect in testis was observed (Fig. 8, Ref. 43). Text in PDF www.elis.sk.

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DNA damaging potential of zinc oxide nanoparticles in human epidermal cells

Cited by 566 publications

References 45 publications

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Green Synthesis of Silver Nanoparticles Using Leaf Extract of Rosmarinus officinalis and Its Effect on Tomato and Wheat Plants

Toxic effect of nano-zinc oxide

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