DNA degradation in nuclei of muscle cells followed by ischemic injury in a rabbit amputation model

Abstract

This study evaluated DNA degradation in nuclei of muscle cells during ischemia in a rabbit limb amputation model. Seven New Zealand rabbits were used. The left rear limb of the animal was amputated. The quadriceps femoris muscle was biopsied, as well as the rabbit carotid arteries. Half of the tissue samples were preserved at a cold temperature (2-4 degrees C), and the other half were kept at room temperature (25 degrees C, global ischemia). Immediately and 4, 8, 12, 16, and 24 h after amputation, muscle tissue samples were stained with the Feulgen reaction and analyzed by an image-analysis system to detect DNA contents in muscle cell nuclei. Samples from carotid arteries were examined with a transmission electron microscope. The results showed that the DNA content in muscle cell nuclei was slightly decreased at 4 and 8 h of global ischemia. At 12 h after global ischemia, the DNA content was found to be significantly decreased (80.03% of baseline). The DNA content dropped to 53.93% of baseline at 16 h of global ischemia. However, in the muscle that was preserved at cold temperature, the DNA contents were only slightly changed along with the ischemia time at 24 h. The DNA changes were confirmed by transmission electron microscopic observations. The present findings could provide evidence for the study of revascularization of ischemic muscle.

Outcome for client-owned domestic rabbits undergoing limb amputation: 34 cases (2000–2009)

“…The motivation for this study was the paucity of information describing outcome information limb amputation for rabbits. A search of the literature identified only limited studies [3][4][5][6][7] involving laboratory rabbits and a single case report 2 of a domestic rabbit with osteosarcoma that did not survive amputation. Only 2 studies 5,7 provided outcome information.…”

“…Rabbits were typically euthanized soon after surgery with no evalu-SMALL ANIMALS/ EXOTIC ation of recovery and return to function. 3,4 The purpose of the study reported here was to retrospectively describe morbidity, survival rate, and survival time of client-owned domestic rabbits undergoing amputation of a thoracic or pelvic limb for any reason.…”

Review: Hemodynamic Studies for Lower Limb Amputation and Rehabilitation

“…From a micro perspective, the occurrence mechanism of muscle atrophy is inseparable from protein metabolism and cell death, therefore blood flow is usually involved in the researches about muscle protein synthesis and degradation or apoptosis. For example, in 2006, Wang et al 80 evaluated DNA degradation in nuclei of muscle cells during ischemia in a rabbit limb amputation model. In 2007, the activation of hypoxia-inducible factor in skeletal muscle cells during ischemia was investigated by Dehne et al 81 A bone marrow cell therapy was performed by Liu et al 82 on a model of severe lower limb ischemia in 2009.…”

¿Es posible utilizar las muestras de colecistectomías con cáncer en investigación?: Calidad del ADN de muestras obtenidas del sistema público y privado de salud

“…Las piezas quirúrgicas provenientes de estas intervenciones son procesadas rutinariamente en los servicios de Anatomía Patológica, muchas veces sin las debidas precauciones para conservar este material en condiciones que pudiesen permitir la realización de estudios posteriores con técnicas de biología molecular. Largos períodos de isquemia fría, fijación inadecuada, procesamiento no estandarizado, almacenamiento en condiciones no óptimas, son sólo algunos de los factores que determinan la pérdida de gran cantidad de dicho material [16][17][18] . La ausencia de bancos de tumores en la mayor parte de los servicios asistenciales, también, ha impedido la recolección de tejidos frescos que pudiesen ser utilizados en investigación a nivel genético-molecular.…”