DNA-Delivery Methods to Produce Transgenic Plants

Darbani, Behrooz; Farajnia, Safar; Toorchi, Mahmoud; Zakerbostanabad, Saeed; Noeparvar, Shahin; Stewart, Diana

doi:10.3923/biotech.2008.385.402

Cited by 34 publications

(16 citation statements)

References 7 publications

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“…Electroporation is a technique in which pulse of high electric field/high voltage (0.5 mA or 25 mV for 15 minutes) is applied to increase the permeability of cell membrane. This method is used to cause some type of structural rearrangement of the cell membrane resulting in a temporary increase in porosity and providing a local driving force for ionic and molecular transport through the pores (Darbani et al, 2008). The most common application of electroporation is in vitro introduction of DNA into cells.…”

Section: Electroporation/electrotransfectionmentioning

confidence: 99%

“…The most common application of electroporation is in vitro introduction of DNA into cells. Physical factors such as transmembrane potential generated by the imposing pulse of electric field, extent of membrane permeation, duration of the permeated state, mode and duration of molecular flow, global and local (surface) concentrations of DNA, form of DNA, tolerance of cells to membrane permeation, and the heterogeneity of the cell population may affect the electrotransfection efficiency of transgenic plant thus produced (Darbani et al, 2008;Hui, 1995;Weaver, 1995).…”

Section: Electroporation/electrotransfectionmentioning

confidence: 99%

“…This method involves a derivation of polyethylene glycol-mediated transformation known as liposome-mediated transformation technique. Liposomes are positively charged lipids and are used for DNA uptake due to their favorable interactions with negatively charged DNA and cell membranes (Darbani et al, 2008). In this approach external DNA is encapsulated in a spherical lipid bilayer (which is known as a liposome) to prepare lipoplexes (Gad et al, 1990).…”

Section: Lipofectionmentioning

confidence: 99%

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Omics and Edible Vaccines

Munshi

Sharma

2018

Omics Technologies and Bio-Engineering

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Section: Electroporation/electrotransfectionmentioning

confidence: 99%

Section: Electroporation/electrotransfectionmentioning

confidence: 99%

Section: Lipofectionmentioning

confidence: 99%

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Omics and Edible Vaccines

Munshi

Sharma

2018

Omics Technologies and Bio-Engineering

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“…Efficient plant gene delivery systems are crucial for understanding gene functions, improving plant quality, and facilitating molecular biofarming (Darbani et al, 2008). Numerous methods have been established to introduce foreign genes into plants using Agrobacterium, gene-gun, and plant viral vector-based systems.…”

Section: Research Reportmentioning

confidence: 99%

Expression of recombinant proteins in plants by using baculovirus vectors

Kim

Park

et al. 2011

Hortic. Environ. Biotechnol.

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Baculovirus has been widely used for the production of numerous recombinant proteins in insect cells. Baculovirus vectors have several advantages, including proper post-translational modification, biosafety, and multiple large gene expression ability. Most insect cell-produced proteins have been expressed by using the baculovirus expression vector system (BEVS) under the control of strong polyhedrin (Polh) or p10 promoters. There has been no report on the expression of recombinant proteins by baculovirus in plant cells. In this study, we used the baculovirus vector to express recombinant green fluorescent protein (GFP) in plants. To investigate the expression of GFP protein by baculovirus in plants, we cloned the gfp gene under the control of Polh promoter or Cauliflower Mosaic Virus (CaMV) 35S promoter to yield the Polh-GFP and 35S-GFP bacmids carrying the GFP expression cassettes, respectively. The presence of Polh-GFP and 35S-GFP expression cassettes in the bacmids was confirmed by polymerase chain reaction (PCR). Subsequently, both the GFP bacmids and GFP baculovirus vectors generated from the bacmid-transfected Sf9 insect cells were inoculated into Nicotiana benthamiana leaves. Confocal microscopy revealed that the gfp gene expression was high in plant leaves at 48 and 72 h after bacmid and baculovirus inoculation. Reverse transcription-PCR (RT-PCR) and fluorescence microscopy confirmed that the gfp genes under the control of Polh or CaMV35S promoters were highly expressed in plant leaves inoculated with 40 L of baculovirus solution. These results suggested that the baculovirus vector can be used to express recombinant proteins in plants. The baculovirus vector-mediated gene delivery and expression system could be used in plant biotechnology for fast and efficient production of recombinant proteins and for molecular virology studies in plants.Additional key words: CaMV 35S promoter, GFP protein, Sf9 insect cell, polyhedrin promoter Hort. Environ. Biotechnol. 52(1):95-104. 2011.

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“…Discovering methods to deliver DNA into plant cells was a key enablement for transformation (Darbani et al, 2008;Nandakumar et al, 2011). Many developments in plant DNA delivery technologies were built on progress in transformation of microbes and animal cell cultures.…”

Section: Direct Dna Deliverymentioning

confidence: 99%

Transgenic Methodologies – Plants

Somers

2014

Encyclopedia of Agriculture and Food Systems

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DNA-Delivery Methods to Produce Transgenic Plants

Cited by 34 publications

References 7 publications

Omics and Edible Vaccines

Omics and Edible Vaccines

Expression of recombinant proteins in plants by using baculovirus vectors

Transgenic Methodologies – Plants

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