DNA detection of JC and BK virus in archival urine cytospin slides

Assis, Patricia Gioia de; Carvalho, Carlos Eduardo; Silva, Marcelo Soares da Mota e; Carvalho, Maria da Glória da Costa

doi:10.1002/jmv.24979

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…It can be observed when urine BKV increases to approximately 4-5 log 10 c/mL [62], when BKV is not amplified in plasma in many cases. However, decoy cells are not specific for the presence of BKV in urine and can be found in JC polyomavirus and adenovirus infection [62][63][64][65].…”

Section: Urine Cytologymentioning

confidence: 99%

BK Virus-Associated Nephropathy after Renal Transplantation

Funahashi

2021

Pathogens

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Recent advances in immunosuppressive therapy have reduced the incidence of acute rejection and improved renal transplantation outcomes. Meanwhile, nephropathy caused by BK virus has become an important cause of acute or chronic graft dysfunction. The usual progression of infection begins with BK viruria and progresses to BK viremia, leading to BK virus associated nephropathy. To detect early signs of BK virus proliferation before the development of nephropathy, several screening tests are used including urinary cytology and urinary and plasma PCR. A definitive diagnosis of BK virus associated nephropathy can be achieved only histologically, typically by detecting tubulointerstitial inflammation associated with basophilic intranuclear inclusions in tubular and/or Bowman’s epithelial cells, in addition to immunostaining with anti-Simian virus 40 large T-antigen. Several pathological classifications have been proposed to categorize the severity of the disease to allow treatment strategies to be determined and treatment success to be predicted. Since no specific drugs that directly suppress the proliferation of BKV are available, the main therapeutic approach is the reduction of immunosuppressive drugs. The diagnosis of subsequent acute rejection, the definition of remission, the protocol of resuming immunosuppression, and long-term follow-up remain controversial.

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Section: Urine Cytologymentioning

confidence: 99%

BK Virus-Associated Nephropathy after Renal Transplantation

Funahashi

2021

Pathogens

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“…Эти клетки идентифицируют по их типичным внутриядерным включениям цвета матового стекла на мазках, окрашенных по методу Папаниколау. Однако decoy-клетки не специфичны для присутствия BKPyV и могут быть обнаружены при JCPyV-инфекции [58]. Стоит также отметить, что образец мочи должен быть исследован в течение 3 ч после сбора, что представляет определенные технические сложности.…”

Section: достоверная дифференциальная диагностикаunclassified

A systematic review. Current understanding of the infection caused by <i>Betapolyomavirus hominis</i>

Prilepskaya

Домонова

2023

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Reactivation of Betapolyomavirus hominis (BKPyV) in kidney and hematopoietic stem cell recipients can lead to serious complications such as BKPyV-associated nephropathy followed by transplant rejection and BKPyV-associated hemorrhagic cystitis. Early diagnosis of the disease is hampering by the possible combination of infection of BKPyV with other post-transplant pathologies and the absence of specific symptoms. Replication of BKPyV is currently the only reliable prognostic sign of the development of long-term consequences, so patient management is basseting on monitoring the concentration of viral DNA. However, consistency between the results of determining the viral load and the development of post-transplant complications associated with BKPyV reactivation cannot be achieving without effective means of standardizing laboratory testing. This review covers the current understanding of the epidemiology; pathogenesis and the clinical features of the disease associated with BKPyV, and also considers in detail the current methods of laboratory diagnosis infection of BKPyV.

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DNA detection of JC and BK virus in archival urine cytospin slides

Cited by 2 publications

References 25 publications

BK Virus-Associated Nephropathy after Renal Transplantation

BK Virus-Associated Nephropathy after Renal Transplantation

A systematic review. Current understanding of the infection caused by <i>Betapolyomavirus hominis</i>

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