DNA-Directed Assembly of Single-Wall Carbon Nanotubes

Chen, Yi; Liu, Haipeng; Ye, Tao; Kim, Junghwa; Mao, Chengde

doi:10.1021/ja072838t

Cited by 122 publications

(81 citation statements)

References 14 publications

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“…Using branched DNA as the main building block (also known as a ''tile'') and cohesive singlestranded DNA (ssDNA) ends to designate the pairing strategy for tile-tile recognition, one can rationally design and assemble complicated nanoarchitectures from specifically designed DNA oligonucleotides. Objects in both two and three dimensions with a large variety of geometries and topologies have been built from DNA with excellent yield (3-9); this development enables the construction of DNA-based nanodevices (10-11) and DNA template directed organization of other molecular species (12)(13)(14)(15)(16)(17)(18)(19). The construction of such nanoscale objects constitutes the basis of DNA nanotechnology.…”

mentioning

confidence: 99%

In vivo cloning of artificial DNA nanostructures

Lin

Rinker

Wang

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

119

106

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Mimicking nature is both a key goal and a difficult challenge for the scientific enterprise. DNA, well known as the genetic-information carrier in nature, can be replicated efficiently in living cells. Today, despite the dramatic evolution of DNA nanotechnology, a versatile method that replicates artificial DNA nanostructures with complex secondary structures remains an appealing target. Previous success in replicating DNA nanostructures enzymatically in vitro suggests that a possible solution could be cloning these nanostructures by using viruses. Here, we report a system where a single-stranded DNA nanostructure (Holliday junction or paranemic cross-over DNA) is inserted into a phagemid, transformed into XL1-Blue cells and amplified in vivo in the presence of helper phages. High copy numbers of cloned nanostructures can be obtained readily by using standard molecular biology techniques. Correct replication is verified by a number of assays including nondenaturing PAGE, Ferguson analysis, endonuclease VII digestion, and hydroxyl radical autofootprinting. The simplicity, efficiency, and fidelity of nature are fully reflected in this system. UV-induced psoralen cross-linking is used to probe the secondary structure of the inserted junction in infected cells. Our data suggest the possible formation of the immobile four-arm junction in vivo.DNA nanotechnology ͉ immobile DNA junction ͉ self-replication ͉ synthetic biology T he notion that DNA is merely the gene encoder of living systems has been eclipsed by the successful development of DNA nanotechnology (1-2). Using branched DNA as the main building block (also known as a ''tile'') and cohesive singlestranded DNA (ssDNA) ends to designate the pairing strategy for tile-tile recognition, one can rationally design and assemble complicated nanoarchitectures from specifically designed DNA oligonucleotides. Objects in both two and three dimensions with a large variety of geometries and topologies have been built from DNA with excellent yield (3-9); this development enables the construction of DNA-based nanodevices (10-11) and DNA template directed organization of other molecular species (12)(13)(14)(15)(16)(17)(18)(19). The construction of such nanoscale objects constitutes the basis of DNA nanotechnology. However, the synthetic scale of oligonucleotides limits the potential applications of DNA nanotechnology, especially when the nanostructure is designed to be made from long ssDNA (Ͼ100 bases). Replicable DNA nanostructures are thus a highly desirable goal to help overcome this barrier.To this end, several possible solutions have been explored. For example, a three-point star motif has been replicated by chemical methods in which the parental nanomotif serves as the template to direct the chemical ligation of nonidentical DNA strands to form the next generation of the nanomotif (20). Another instance involves a 1.7-kb ssDNA that was used to assemble a DNA octahedron (together with five short strands); this molecule was cloned in a plasmid in bacteria (9). A nicking end...

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mentioning

confidence: 99%

In vivo cloning of artificial DNA nanostructures

Lin

Rinker

Wang

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

119

106

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“…Similar to proteins, DNAs and RNAs could be attached to carbon nanotubes noncovalently by adsorption [30][31][32] and covalently in carbodiimide-activated coupling reactions either directly [33,34] or through a bifunctional linker. [35] For example, Hamers and coworkers used the bifunctional linker approach to functionalize sidewalls of carbon nanotubes in several steps, starting by the reaction of a 4-nitrobenzenediazonium salt, then the electrochemical reduction of -NO 2 to -NH 2 for reaction with a heterobifunctional cross-linker to introduce a maleimide group, followed by coupling with 5 0 -thiol-modified single strand DNA (ssDNA).…”

Section: Dnas/rnasmentioning

confidence: 99%

“…In an exploration on DNAdirected multicomponent self-assembly of carbon nanotubes and gold nanoparticles, [31][32][33] ssDNA was first grafted onto the nanotube surface via direct wrapping or carbodiimide-activated coupling to form ssDNA-SWNT conjugates and then hybridized with complimentary DNA sequences grafted (via the specific thiol-gold interaction) on gold nanoparticles. The DNA-directed assembly was found to be reversible: formation at low temperature and dissociation at high temperature.…”

Section: Dnas/rnasmentioning

confidence: 99%

Advances in Bioapplications of Carbon Nanotubes

et al. 2009

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This progress report provides an overview on recent advances in bioapplications of carbon nanotubes including the chemical modification of carbon nanotubes, targeting specifically their covalent and noncovalent conjugations with a variety of biological and bioactive species (proteins and peptides, DNAs/RNAs, and carbohydrates). Furthermore, the significant recent development and progress in the use of carbon nanotubes for biosensors, drug and other delivery systems, bioimaging, etc. and in the understanding of in vivo biodistribution and toxicity of carbon nanotubes are reported.

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“…高超 [7] 制备了聚甲基丙烯酸甲酯修饰的碳纳米管, 发现这种修饰的多壁碳纳米管可以在金、云母、石英和硅表面上自组装成一些形态各异的微观结构, 他认为在溶剂挥发过程中, 高分子链的相分离推动并且稳定了具有刚性的碳纳米管的自组装. Chen 等 [8] 利用…”

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Self-Assembly of Polymer-Functionalized Carbon Nanotubes Induced by Polymer Microphase Separation

Zheng¹,

Gao²,

Ding³

et al. 2013

Chin. J. Org. Chem.

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It is of importance for the application of carbon nanotubes in composite materials via supramolecular self-assembly of surface-functionalized carbon nanotubes. Here the self-assembly of the mixture of poly(4-vinylpyridine) functionalized multi-walled carbon nanotubes and carboxyl-containing graft copolymer, poly(acrylic acid)-g-poly(methyl methacrylate) is reported. Because of the hydrogen bond between the pyridine nitrogen and the carboxyl of the graft copolymer, carbon nanotubes were induced to form organized structure during the polymer microphase separation process, which afforded a kind of honeycomb suprastructure containing carbon nanotubes.

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DNA-Directed Assembly of Single-Wall Carbon Nanotubes

Cited by 122 publications

References 14 publications

In vivo cloning of artificial DNA nanostructures

In vivo cloning of artificial DNA nanostructures

Advances in Bioapplications of Carbon Nanotubes

Self-Assembly of Polymer-Functionalized Carbon Nanotubes Induced by Polymer Microphase Separation

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