DNA Distribution Patterns of Preneoplastic Cells and Their Interpretation

Weiß, H.; Wildner, G.; Gütz, Herbert; Ebeling, K; Steinhoff, G.; Tanneberger, S

doi:10.1159/000225553

Cited by 20 publications

(7 citation statements)

References 21 publications

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“…Hedley et al [17] and Schutte [16] devised new methods using paraffin-embedded tissue, which have made retrospective studies possi ble and allowed more accuracy. Evidence is now accumulating that DNA aneuploidy is a marker of prognosis in colorectal adenocarci noma as well as in other gastrointestinal tu mors, such as gastric cancer [ 19] and gastroin testinal leiomyosarcoma [20], In the field of colorectal cancer, DNA aneuploidy as mea sured by FCM is present in about 60% of colorectal adenocarcinomas [21][22][23], Studies in premalignant conditions such as ulcerative colitis [12] and chronic atrophic gastritis [24] have shown that DNA aneuploidy can arise prior to the onset of invasive malignancy.…”

Section: Discussionmentioning

confidence: 99%

Histopathologic and Flow Cytometric Analysis of Colorectal Polyps Resected at Endoscopy

Kimura

Kanno²,

Takamura³

et al. 1993

Eur Surg Res

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Forty-seven colorectal polyps of various histologic types were studied using retrospective flow cytometric (FCM) DNA analysis to determine the prevalence of aneuploid cell populations and whether they were associated with any particular histologic features. Overall, 6 of 47 polyps (12.8%) contained DNA aneuploid cells, comprising 4 adenomatous polyps, 1 of 12 with mild atypia (8.3 %), 2 of 19 with moderate atypia (10.5 %) and 1 of 4 with severe atypia (25.0%), and 2 of 4 polyps (50.0%) with histologic features of carcinoma in situ (CIS). The incidence of DNA aneuploid cells in CIS was significantly higher than that of all adenomatous polyps (p = 0.043). All of the 8 hyperplastic polyps were diploid. The detection of aneuploidy as a marker of increased risk for malignant transformation was possible even in colorectal adenomas measuring ≤ 10 mm. These results show that retrospective FCM analysis can detect DNA aneuploidy in adenomatous polyps and that DNA aneuploidy may occur before histologic evidence of invasive carcinoma.

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Section: Discussionmentioning

confidence: 99%

Histopathologic and Flow Cytometric Analysis of Colorectal Polyps Resected at Endoscopy

Kimura

Kanno²,

Takamura³

et al. 1993

Eur Surg Res

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“…Also dormant 'D' cells as described by Darzynkiewicz et al [6] should be taken into con sideration for the explanation of these peaks. The small extra peaks observed in the part of the histo gram which includes cells in S-phasc should also be seen in connection with dormant cells or with phagocytizing cells [37], Summarizing the results presented here and taking the DNA distribution pat tern as a parameter for characterizing and describing the biological value of pathological alteration, we have to attribute an equal biological potential to tu bular and tubulo-villous adenomas. The question re garding the value of an abnormal DNA distribution pattern for prediction of a malignant transformation in a single case remains open.…”

Section: Discussionmentioning

confidence: 62%

“…An only suggested scattering or splitting of the 4C peak was seen in approximately the same frequency (about 20%) in tubular and tubulovillous adenomas without dysplasia. These scattered shapes were also found in cells from chronic atrophic gastric mucosa [37] and gastric mucosal cells originat ing from the surrounding of hyperplastic polyps [25]. The reason for these shapes is supposed to be a nonuniform chromatin structure of the cells collected in these 4C peaks.…”

Section: Discussionmentioning

confidence: 82%

“…Two or more DNA lines were found in about half of large bowel carcinomas [26]. Correlations be tween the frequency of aneuploid DNA cell lines and the histological grading were observed in tumors of the urinary bladder [34], In cases of precancer of the urinary bladder [34], of the uterine cervix [7,27,32,37,38], and of bronchial cells [21] aneuploidy was found too. For esophageal cells the transition from mild dysplasia to severe dysplasia was shown to be ac companied by a broad dispersion of the DNA values without prominent peaks; the latter were specific for invasive carcinomas [20].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Human Adenomatous Polyps of the Colorectal Bowel by Means of DNA Distribution Patterns

Weiß¹,

Wildner²,

Jacobasch³

et al. 1985

Oncology

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64 human adenomatous polyps of the colorectal bowel were investigated by histological examination and by flow cytometrical analysis providing DNA distribution patterns. The histological investigation yielded tubular adenomas (TA) in 41 and tubulo-villous adenomas (TVA) in 23 cases. Epithelial dysplasia or carcinomatous degeneration was found in 23 TA and in 11 TVA. Cell cycle stage analysis revealed no different values for TA and TVA without dysplasia, neither for TA and TVA with mild to moderate dysplasia. TVA with severe dysplasia exhibited a significantly higher proportion of cells with G₂M phase DNA content than cases without dysplasia or mild to moderate dysplasia. TA and TVA without dysplasia were characterized by diploid DNA distribution patterns. TA with dysplasia showed aneuploid DNA stem lines in 4 of 20 cases, carcinomas arisen from TA in all 4 cases investigated. TVA with dysplasia were characterized by aneuploid DNA stem lines in 1 of 8 cases, carcinomas arisen from TVA in all 4 cases examined. It may be concluded that the DNA distribution patterns do not attribute different biological values to TA and TVA, 2 groups of adenomatous polyps which are characterized by different histological criteria.

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“…The use of gastric endoscopies as a source of cells for cytometric determinations has been reported by other authors (4, 19). In some studies, chronic atrophic gastri-tis has been also discussed (22,23). However, in the latter reports ploidy level and "shape" of the DNA content pattern have been jointly used as cytometric indicators.…”

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confidence: 95%

Cytometrically determined relative DNA content as an indicator of neoplasia in gastric lesionis,

Teodori¹,

Capurso²,

Cordelli³

et al. 1984

Cytometry

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Cellular samples from human gastric endoscopic biopsies were analysed in order to detect possible DNA content alterations as markers of cancerous and precancerous lesions of the digestive tract. Samples were derived from the stomach of normal donors (17 cases), and from patients clinically classified as affected by stomach adenocarcinoma (18 cases), chronic atrophic gastritis (20 cases), or other non‐neoplastic lesions (17 cases). Sample processing was performed by mechanical and enzymatic treatment to obtain monodispersed cells. Staining for flow cytometric analysis was achieved with ethidium bromide and mithramycin. Samples from normal donors constantly exhibited a single cell population with diploid DNA content. All but three neoplastic specimens exhibited both a diploid and an aneuploid cell subpopulation, with the DNA index of the aneuploid peak ranging from 1.10 to 1.85 (except a single instance with a value of 3. 13). The presence of a recognizable aneuploid subpopulation was also observed in 9 out of 20 chronic atrophic gastritis specimens. Such aneuploidy is similar to that observed for the adenocarcinoma, even if the fraction of aneuploid cells appears to be generally higher in the tumor than in the gastritis cases. All other cases of gastritis and of non‐neoplastic disease exhibited diploid cells only. The meaning of aneuploidy in some gastritis specimens is a phenomenon not yet fully explained. Still, aneuploidy appears to be a useful marker for recognizing the presence of suspect malignant cells in gastric lesions.

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DNA Distribution Patterns of Preneoplastic Cells and Their Interpretation

Cited by 20 publications

References 21 publications

Histopathologic and Flow Cytometric Analysis of Colorectal Polyps Resected at Endoscopy

Histopathologic and Flow Cytometric Analysis of Colorectal Polyps Resected at Endoscopy

Characterization of Human Adenomatous Polyps of the Colorectal Bowel by Means of DNA Distribution Patterns

Cytometrically determined relative DNA content as an indicator of neoplasia in gastric lesionis,

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