DNA–DNA interaction on dendron-functionalized sol–gel silica films followed with surface plasmon fluorescence spectroscopy

Kwon, Sung Hong; Hong, Bong Jin; Park, Hye Young; Knoll, Wolfgang; Park, Chong-Yun

doi:10.1016/j.jcis.2007.01.041

Cited by 17 publications

(14 citation statements)

References 35 publications

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“…In this regard, attomolar sensitivity in immunoassays based on SPFS has been reported 40. SPSF has also been used for DNA hybridization41–43 and protein detection 44…”

Section: Advanced Chemiluminescence Concepts: III Surface Plasmonmentioning

confidence: 99%

“…In this regard, attomolar sensitivity in immunoassays based on SPFS has been reported. 40 SPSF has also been used for DNA hybridization [41][42][43] and protein detection. 44 Our research laboratory recently reported a companion technique called surface plasmon coupled chemiluminescence (SPCC), which is based on the interactions of chemiluminescence emission with metal thin films.…”

Section: Advanced Chemiluminescence Concepts: III Surface Plasmon Cou...mentioning

confidence: 99%

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Metal-enhanced chemiluminescence: advanced chemiluminescence concepts for the 21st century

2009

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Chemiluminescent based detection is entrenched throughout the Biosciences today, such as in blotting, analyte and protein quantification and detection. While the biological applications of chemiluminescence are forever growing, the underlying principles of using a probe, an oxidizer and a catalyst (biological, organic or inorganic) have remained mostly unchanged for decades. Subsequently, chemiluminescence based detection is fundamentally limited by the classical photochemical properties of reaction yield, quantum yield, etc. However, for the last 5 years, a new technology has emerged which looks set to fundamentally change the way we both think about and use chemiluminescence today. Metal surface plasmons can amplify chemiluminescence signatures, while low-power microwaves can complete reactions within seconds. In addition, thin metal films, can convert spatially isotopic chemiluminescence into directional emission. In this timely forward looking tutorial review, we survey what could well be the next-generation chemiluminescent based technologies. ChemiluminescenceChemiluminescence is a useful analytical tool for the detection and quantification of a wide variety of biological materials such as cells, 1 microorganisms, 2, 3 proteins, 4 DNA 5 , RNA 6,7 and also other analytes. 8,9 For detailed information on the specific applications of chemiluminescence, the reader is referred to the references given here [1][2][3][4][5][6][7][8][9] The usefulness of chemiluminescence is due to its simplicity and the absence of unwanted background luminescence. In chemiluminescence-based detection, no excitation source and no optical filters are required as compared to other optical techniques such as fluorescence and phosphorescence spectroscopy. 10 Chemiluminescence emission is generated by photochemical reactions and is directly related to the concentration of the reactants. The chemical reactions involve the oxidation of an organic dye by a strong oxidizing agent in the presence of a catalyst (chemical or biological). The most commonly used dyes are luminol and acridan, 11 which are not luminescent in the ground state (before an oxidation reaction, Figure 1A). The oxidation of luminol or acridan with hydrogen peroxide (oxidizing agent) in the presence of a catalyst results in the conversion of the ground state of luminol or acridan into an activated state (chemically induced electronic excited states). A strong blue emission (at 450 nm wavelength) can be observed as a as a result of the decay of the excited states back to the ground state. Chemiluminescence solution emission can last from seconds to hours depending on the quantity of reacting species ( Figure 1B). The versatility and simplicity of chemiluminescence has also led to household products/toys such as "glow sticks", which typically employ organic dyes which can emit three primary colors: red, green and blue ( Figure 1C). On the other hand, while chemiluminescence is a versatile tool several factors limit the *Corresponding Authorgeddes@umbi.umd.edu. eff...

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“…In this regard, attomolar sensitivity in immunoassays based on SPFS has been reported 40. SPSF has also been used for DNA hybridization41–43 and protein detection 44…”

Section: Advanced Chemiluminescence Concepts: III Surface Plasmonmentioning

confidence: 99%

Section: Advanced Chemiluminescence Concepts: III Surface Plasmon Cou...mentioning

confidence: 99%

Metal-enhanced chemiluminescence: advanced chemiluminescence concepts for the 21st century

2009

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“…These differences may be due to the nature of regular and wide interprobe spaces of dendron slides. 21 – 23 These results indicate that the addition of cy3-dNTPs during hybridization can significantly increase the intensity of signals on dendron slides.…”

Section: Resultsmentioning

confidence: 78%

“…To solve the spacing problem, dendron-modified slides (dendron slides) have been developed; they provide regular and wide interprobe spaces, referred to as mesospacing. 21 – 23 The mesospacing of dendron slides could provide higher selectivity and sensitivity than those of other microarray slides, and discriminate the SNPs. 24 …”

Section: Introductionmentioning

confidence: 99%

Direct Detection of Drug-Resistant Hepatitis B Virus in Serum Using a Dendron-Modified Microarray

Kim¹,

Kang²,

Hur³

et al. 2018

Gut and Liver

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Background/AimsDirect sequencing is the gold standard for the detection of drug-resistance mutations in hepatitis B virus (HBV); however, this procedure is time-consuming, labor-intensive, and difficult to adapt to high-throughput screening. In this study, we aimed to develop a dendron-modified DNA microarray for the detection of genotypic resistance mutations and evaluate its efficiency.MethodsThe specificity, sensitivity, and selectivity of dendron-modified slides for the detection of representative drug-resistance mutations were evaluated and compared to those of conventional slides. The diagnostic accuracy was validated using sera obtained from 13 patients who developed viral breakthrough during lamivudine, adefovir, or entecavir therapy and compared with the accuracy of restriction fragment mass polymorphism and direct sequencing data.ResultsThe dendron-modified slides significantly outperformed the conventional microarray slides and were able to detect HBV DNA at a very low level (1 copy/μL). Notably, HBV mutants could be detected in the chronic hepatitis B patient sera without virus purification. The validation of our data revealed that this technique is fully compatible with sequencing data of drug-resistant HBV.ConclusionsWe developed a novel diagnostic technique for the simultaneous detection of several drug-resistance mutations using a dendron-modified DNA microarray. This technique can be directly applied to sera from chronic hepatitis B patients who show resistance to several nucleos(t)ide analogues.

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“…A 50 nm silicon monoxide layer is deposited on the source-drain gold electrodes as a spatial spacer as well as an electrical insulator and chemical barrier for electric characterization. And a sol–gel silica film with a silicon oxide complex (SiO x ) element is also delivered onto the gold surface to covalently bind the cone-shaped dendrimer unit for a highly sensitive and specific DNA analysis . Besides the gold substrate, a silicon-based alloy is deposited onto a silver substrate for SPFS measurements .…”

Section: Practical Application Of Surface Plasmon-assisted Fluorescencementioning

confidence: 99%

Surface Plasmon-Assisted Fluorescence Enhancing and Quenching: From Theory to Application

Jiang

Gou

et al. 2021

ACS Appl. Bio Mater.

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The integration of surface plasmon resonance and fluorescence yields a multiaspect improvement in surface fluorescence sensing and imaging, leading to a paradigm shift of surface plasmonassisted fluorescence techniques, for example, surface plasmon enhanced field fluorescence spectroscopy, surface plasmon coupled emission (SPCE), and SPCE imaging. This Review aims to characterize the unique optical property with a common physical interpretation and diverse surface architecture-based measurements. The fundamental electromagnetic theory is employed to comprehensively unveil the fluorophore−surface plasmon interaction, and the associated surfacemodification design is liberally highlighted to balance the surface plasmon-induced fluorescence-enhancement efforts and the surface plasmon-caused fluorescence-quenching effects. In particular, all types of surface structures, for example, silicon, carbon, protein, DNA, polymer, and multilayer, are systematically interrogated in terms of component, thickness, stiffness, and functionality. As a highly interdisciplinary and expanding field in physics, optics, chemistry, and surface chemistry, this Review could be of great interest to a broad readership, in particular, among physical chemists, analytical chemists, and in surface-based sensing and imaging studies.

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DNA–DNA interaction on dendron-functionalized sol–gel silica films followed with surface plasmon fluorescence spectroscopy

Cited by 17 publications

References 35 publications

Metal-enhanced chemiluminescence: advanced chemiluminescence concepts for the 21st century

Metal-enhanced chemiluminescence: advanced chemiluminescence concepts for the 21st century

Direct Detection of Drug-Resistant Hepatitis B Virus in Serum Using a Dendron-Modified Microarray

Surface Plasmon-Assisted Fluorescence Enhancing and Quenching: From Theory to Application

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