DNA-DNA reassociation studies on the genus Pedomicrobium

Pedomicrobium americanum sp. nov. and Pedomicrobium australicum sp. nov. from Aquatic Habitats, Pedomicrobium gen. emend., and Pedomicrobium ferrugineum sp. emend.

“…The methods used for cell wall disintegration and deoxyribonucleic acid (DNA) extraction and purification have been described previously (12). Shearing of DNA and radioactive labeling were carried out as described by Gebers et al (10,11). DNA reassociation procedures and S1 nuclease treatment were performed as described previously (10).…”

“…Subsequently, these strains were identified as Pedomicrobium ferrugineum and Pedomicrobium manganicum, and an emended description was given by Gebers (7). Further investigations demonstrated the genetic relatedness between these Pedomicrobium strains and their relationship to other genera of hyphal, budding bacteria (9)(10)(11)(12)20). In these studies, five isolates from aquatic habitats in North America and Australia (12) proved to be two new Pedomicrobium species.…”

Effect of water velocity on the early development of manganese-depositing biofilm in a drinking-water distribution system

“…On the basis of morphological similarity Tyler and Marshall suggested that Pedomicrobium was an invalid genus [16,36,37,39]. However, subsequent research has supported the existence of Pedomicrobium as a separate genus on morphological, physiological and genetic grounds [10,[40][41][42][43][44][45][46].…”

Deoxyribonucleic Acid Base Compositions and Nucleotide Distributions of 65 Strains of Budding Bacteria

“…strain B-522 (Mr, 3.1 X lo9) (34) and Rhodomicrobiurn vannielii RM5 (Mr, 2.1 X lo9) (40). DNA-DNA base sequence homologies have been reported for a number of Hyphomicrobium strains and other budding bacteria (33), for Prosthecomicrobium, Ancalomicrobium, and Hyphomicrobiurn (33, and for Pedomicrobium species (11). Ribosomal ribonucleic acid-DNA hybridizations have been performed only between Hyphomicrobiurn sp.…”

“…Disintegration of the bacterial cell walls was achieved by various procedures ( Table 1). The enzyme A method was a modification of a previously described procedure (10,11); proteinase K (100 pglml; E. Merck AG, Darmstadt,) was used instead of pronase E. The enzyme B, enzyme C, and enzyme D procedures were modifications of the enzyme A method; the cell pellets were suspended not in Tris-MgC12-KCl buffer but in 0.05 M Tris (pH 8), 0.05 M Tri s-0.05 M e th ylenediamine t etraace tate (EDTA)-0 .01 M NaCl (pH 8), and saline-EDTA (28), respectively. In the enzyme E method, the cells were suspended in 9.6 ml of Tris-MgCI2-KC1 buffer; then a solution containing 2 mg of sodium dodecyl sulfate per ml and 1 mg of proteinase K were added, and the mixture was incubated at 60 or 37°C for a maximum of 45 min.…”