DNA electrochemical biosensor based on thionine-graphene nanocomposite

Zhu, Limei; Luo, Liqiang; Wang, Zhenxin

doi:10.1016/j.bios.2012.03.026

Cited by 148 publications

(34 citation statements)

References 41 publications

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“…It may get a hint from the thermal reduction of GO in deionized water [62]. Differential pulse voltammetry (DPV) also represents a sensitive method for DNA detection based on the electrochemical signals of intercalators or tags, such as adriamycin [63], daunomycin [64,65], methylene blue [66], and Au [67]. Without these intercalators or tags, an ultrasensitive and selective DNA detection by using stand-displacement DNA polymerization and parallel-motif DNA triplex system as dual amplifications was realized on ferrocene-appended poly(ethyleneimine)/ graphene-mesoporous silica-gold nanoparticle hybrids (Fc-PEI/GSGHs) n [68].…”

Section: Electrochemical Assaymentioning

confidence: 99%

Graphene for DNA Biosensing

Han

et al. 2014

SpringerBriefs in Molecular Science

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Graphene (or GO) is an excellent candidate for biomolecules anchoring and detection due to its large surface area (up to 2,630 m 2 /g) and unique sp 2 (sp 2 /sp 3 )-bonded network. According to the binding affinity difference between single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) to graphene sheet, GO has been successfully adopted as a platform to discriminate DNA sequences. Fluorescent, electrochemical, electrical, surface-enhanced Raman scattering (SERS) and other methods have been utilized to achieve the sensitive, selective, and accurate DNA recognition. Both theoretical and experimental results illustrate that ssDNA sequences are adsorbed on the surface of graphene sheet with all nucleobases lying nearly flat. DNA or RNA sequencing through graphene nanopore, nanogap, and nanoribbon has also attracted much interest because it is a label-free, amplification-free, and single-molecule approach that can be scaled up for high-throughput DNA or RNA analysis. Meanwhile, miRNA detection is achieved by forming DNA-miRNA duplex helixes, and strong emission is observed due to the poor interaction between the helix and GO.Keywords DNA sequencing Á miRNA detection Á Fluorescent spectroscopy Á Electrochemical method Á Electrical method Á Graphene nanopore Á Graphene nanogap Á Graphene nanoribbon Investigation of DNA and Graphene Binding InteractionThe large surface area (up to 2,630 m 2 /g) and unique sp 2 (sp 2 /sp 3 )-bonded network make graphene (GO) an excellent candidate for biomolecules anchoring and detection. Taking DNA for the first example, various strategies have been adopted to nucleic acid sequence recognition [1][2][3][4][5][6][7][8][9][10]. To better illustrate the interaction, how nucleobases interact with graphene should be addressed, which may be inspired by the interaction of nucleobases with CNTs [11-13] and highly oriented pyrolytic graphite (HOPG) [14].

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Section: Electrochemical Assaymentioning

confidence: 99%

Graphene for DNA Biosensing

Han

et al. 2014

SpringerBriefs in Molecular Science

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“…Graphene-based bioaffinity electrochemical biosensors 4. Graphene-based DNA electrochemical sensors [123] 5. Graphene-based electrochemical immunosensors [124] 6.…”

Section: Graphenementioning

confidence: 99%

Biomolecules and Pure Carbon Aggregates: An Application Towards “Green Electronics”

Srivastava¹

2018

Green Electronics

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Abstract"Green electronics" is a novel scientific term which aims to identify the compounds of natural origin (economically safe and biodegradable) and establish economically efficient route for production of synthetic materials. The purpose of green electronics is to create path for the production of human and environmental friendly electronics and the integration of electronics with living tissue in particular. These researches may help to fulfill not only the organic electronics to deliver low cost energy efficient materials and devices, but also achieve unimaginable functionalities for electronics. In this chapter we have considered the molecular electronic devices biomolecules: deoxyribonucleic acid (DNA) and pure carbon aggregates: (carbon nanotubes (CNTs)/graphene), their properties and applications.

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“…Now, it has become popular to explore the electrochemical properties of GO at electrode surfaces. These GO-based electrodes have been demonstrated to be useful for practical electrochemical applications [24,25].…”

Section: Introductionmentioning

confidence: 99%

Determination of trypsin activity using a gold electrode modified with a nanocover composed of graphene oxide and thionine

et al. 2015

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We report on an electrochemical method for the determination of the activity of trypsin. A multi-functional substrate peptide (HHHAKSSATGGC-HS) is designed and immobilized on a gold electrode. The three His residues in the N-terminal are able to recruit thionine-loaded graphene oxide (GO/thionine), a nanocover adopted for signal amplification. Once the peptide is cleaved under enzymatic catalysis by trypsin (cleavage site: Lys residue), the His residues leave the electrode, and the GO/thionine cannot cover the peptidemodified electrode anymore. Thus, the changes of the electrochemical signal of thionine, typically acquired at a voltage of -0.35 V, can be used to determine the activity of trypsin. A detection range of 1×10 −4 to 1 U, with a detection limit of 3.3×10 −5 U, can be achieved, which is better than some currently available methods. In addition, the method is highly specific, facile, and has the potential for the detection of trypsin-like proteases.

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DNA electrochemical biosensor based on thionine-graphene nanocomposite

Cited by 148 publications

References 41 publications

Graphene for DNA Biosensing

Graphene for DNA Biosensing

Biomolecules and Pure Carbon Aggregates: An Application Towards “Green Electronics”

Determination of trypsin activity using a gold electrode modified with a nanocover composed of graphene oxide and thionine

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