DNA extraction method from bones using Maxwell® 16

Vlahović, Maja; Kubat, Milovan

doi:10.1016/j.legalmed.2012.04.004

Cited by 17 publications

(3 citation statements)

References 8 publications

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“…In terms of skeletal remains, bone powders are the traditional type of bone samples used for DNA extraction (Amory, Huel, Bilić, Loreille, & Parsons, 2012; Karija Vlahović & Kubat, 2012; Latham & Miller, 2019; Zupanič Pajnič et al, 2016). Bone pulverization is important as it maximizes the surface area for recovery of DNA from the hydroxyapatite matrix.…”

Section: Dna Extraction From Human Remains and Non‐conventional Samplesmentioning

confidence: 99%

Recent trends and developments in forensic DNA extraction

Chong

Thong

Syn

2020

WIREs Forensic Science

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The extraction of DNA is a fairly standard procedure routinely undertaken in biological academic and research settings. In a forensic DNA context, DNA extraction is the crucial first step toward the generation of DNA profiles used to support convictions or exonerations in the criminal justice system. However, it is often complicated by the additional challenge of yielding sufficient quantities of clean DNA that is free from environmental inhibitors, from a wide spectrum of sample types which may contain limited quantities of biological material. While improvements in methods and technology over the decades have sped up and simplified the process of DNA extraction, several issues remain to be fully addressed—such as the incomplete separation of spermic DNA from sperm‐epithelial cell mixtures, increasing demands for ever‐faster DNA results, or even on‐scene DNA processing, and processing of difficult samples such as skeletal remains or samples contaminated by Chemical, Biological, or Radiological (CBR) agents. These challenges are often compounded by high work volumes and limited quantities of starting material. As such, this review focuses on recent trends and developments in four areas of forensic DNA extraction: (a) analysis of sexual assault evidence which often represents a significant portion of a forensic DNA laboratory's casework, (b) use of portable rapid DNA instruments which has gained much traction among law enforcement in recent times, (c) DNA extraction from difficult and CBR‐contaminated samples which, albeit rare, would be essential in a DNA laboratory's toolbox in the event of an incident, and (d) bypassing DNA extraction altogether. This article is categorized under: Forensic Biology > Forensic DNA Technologies

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Section: Dna Extraction From Human Remains and Non‐conventional Samplesmentioning

confidence: 99%

Recent trends and developments in forensic DNA extraction

Chong

Thong

Syn

2020

WIREs Forensic Science

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“…Thereafter, 300 µL of clear lysate sample was transferred to the reagent cartridge which was placed in the Maxwell ® 16 for automated DNA purification. This technique has been used to extract DNA from samples of diverse origin including black flies [ 31 , 33 , 34 , 35 ]. Moreover, the sensitivity and relative effectiveness of the method was evaluated in comparison with the phenol chloroform method—gold standard of DNA extraction for entomological surveillance in Latin America—for the detection of O. volvulus in pools of black flies, with results indicating that the automated method is as sensitive as phenol chloroform method, nontoxic, and rapid [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon

et al. 2020

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The enormity of the public health burden of onchocerciasis motivated the creation of various large-scale control programs that have depended principally on mass treatment of endemic communities with ivermectin for the elimination of the disease. Parasitological evaluation of Onchocerca species in the West Region of Cameroon indicates significant progress in the interruption of parasite transmission in some communities under ivermectin treatment. However, to verify the complete elimination of onchocerciasis, entomological assessment through O-150 PCR poolscreen of black flies is mandatory. Thus, in the present study, we assessed transmission of Onchocerca species using an O-150 PCR technique to screen pools of black flies—in seven onchocerciasis endemic communities (Makouopsap, Bankambe, Lemgo, Tsesse, Ndionzou, Kouffen, and Bayon) in Cameroon. Two thousand black flies were assessed—in each community—for the presence of Onchocerca species DNA. Our results show that the frequency of infective flies was 0.6% in Makouopsap and 0.0% in the other communities. On the other hand, the frequency of infected flies was 0.8% in Makouopsap, 0.2% in Bankambe, 0.1% in Bayon, and 0.0% in Lemgo, Tsesse, Ndionzou, and Kouffen. These results provide entomologic evidence for continuous transmission of Onchocerca species in Makouopsap, risk of active transmission in Bankambe, and Bayon, and a suppressed transmission in the four other studied communities.

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“…Although several methods of DNA extraction have been reported, a standard procedure for DNA extraction from old specimens has not yet been established (18,19). Although the PrepFiler TM BTA Forensic DNA Extraction Kit is designed for use with challenging forensic samples (e.g., bones, teeth, cigarette butts, chewing gum, envelope covers, and tapes) (20), this method can extract DNA from only 50 mg of bone or tooth powder at one time.…”

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confidence: 99%

A Simple and Efficient Method of Extracting DNA from Aged Bones and Teeth

Liu

Zhang

et al. 2017

Journal of Forensic Sciences

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DNA is often difficult to extract from old bones and teeth due to low levels of DNA and high levels of degradation. This study established a simple yet efficient method for extracting DNA from 20 aged bones and teeth (approximately 60 years old). Based on the concentration and STR typing results, the new method of DNA extraction (OM) developed in this study was compared with the PrepFiler™ BTA Forensic DNA Extraction Kit (BM). The total amount of DNA extracted using the OM method was not significantly different from that extracted using the commercial kit (p > 0.05). However, the number of STR loci detected was significantly higher in the samples processed using the OM method than using the BM method (p < 0.05). This study aimed to establish a DNA extraction method for aged bones and teeth to improve the detection rate of STR typing and reduce costs compared to the BM technique.

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DNA extraction method from bones using Maxwell® 16

Cited by 17 publications

References 8 publications

Recent trends and developments in forensic DNA extraction

Recent trends and developments in forensic DNA extraction

Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon

A Simple and Efficient Method of Extracting DNA from Aged Bones and Teeth

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