DNA extraction using bacterial magnetic particles modified with hyperbranched polyamidoamine dendrimer

Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation

“…Grüttner et al reported MNPs functionalized with chelating groups on the surface to bind lanthanides and actinides in order to remove radionuclides from nuclear wastes [24]. Magnetically assisted processes have been reported to purify water [25] and MNPs have been proven useful in protein immobilization and purification and DNA extraction [23,29]. The main advantage of magnetite nanoparticles is that they can be easily recovered from the reaction media by magnetic separation.…”

Efficient DNA release from PAMAM dendrimer-modified superparamagnetic nanoparticles for DNA recovery

“…16,19 The solid support first used nanoparticles that had been modified with an amino-silane monolayer. These particles had a low DNA adsorption capacity and low particle dispersivity, although the DNA release was efficient.…”

“…17 The capacity of the amine-modified nanoparticle was then improved by covering it with PAMAM using divergent synthesis. 15,16 However, the PAMAM-modified particles had two problems: controlling the amine numbers on the particles was difficult using the divergent method, and the DNA-release efficiency was low, with a high amino-group density for high dispersivity. We recently developed the self-assembly dendronization method using thiol core, functionalized PAMAM dendrons as a convenient method for PAMAM modification, which allowed the PAMAM-MNPs to be fabricated with sufficient reproducibility.…”

“…They have been used in various biotechnological applications, including cell separation, 2-4 immunoassays, [5][6][7] DNA detection, [8][9][10][11][12] and DNA recovery. [13][14][15][16][17][18] We have developed various types of amine-modified MNPs and have applied them to automated DNA recovery. 13,15,18 This development was based on the electrostatic interactions between the amino groups on the MNPs and the phosphate groups from nucleic acids.…”

“…18 The DNA binding capability of amine-modified MNPs was significantly improved by modifying them with polyamidoamine (PAMAM) dendrons. 16 The high amine density increased the DNA adsorption capacity of the PAMAM-modified MNPs (PAMAMMNPs). In addition, the dispersivity of the MNPs in a solution was increased with the high amino density, generating a repulsive force among the PAMAM-MNPs.…”