DNA Finger Printing of &amp;lt;i&amp;gt;Prosopis cineraria&amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;Prosopis juliflora&amp;lt;/i&amp;gt; Using ISSR and RAPD Techniques

Elmeer, Khaled; Al-Malki, Ameena

doi:10.4236/ajps.2011.24062

Cited by 13 publications

(10 citation statements)

References 41 publications

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“…ISSR-polymerase chain reaction (PCR) is a technique that involves the use of microsatellite sequences as primers in PCR to generate multi-locus markers. ISSR markers are highly polymorphic and are useful in studies of genetic diversity, phylogeny, genetic marking, genome mapping, and evolutionary biology (Elmeer and Almalki, 2011).…”

Section: Introductionmentioning

confidence: 99%

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Hsie¹,

Brito²,

Nova³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Sugarcane culture is an important source of income for the Brazilian economy. The aim of this study was to identify somaclonal variation in sugarcane varieties RB943365 and RB92579 arising from micropropagation using inter-simple sequence repeat (ISSR) DNA markers. The evaluated plants were generated from the in vitro propagation of shoot tips grown in MS medium supplemented with vitamins, myoinositol, glycine, and sucrose, without the use of growth regulators. Fifteen consecutive subcultures with intervals of 14 days were carried out, and DNA was extracted from young leaves obtained from each of the subcultures. The DNA was amplified with ISSR markers and separated by electrophoresis on 2% agarose gels. No evidence of polymorphism was observed in subcultures of the varieties analyzed, suggesting the absence of somaclonal variants. In this way, the ISSR marker was efficient at analyzing somaclonal variation, and in vitro propagation of sugarcane can be considered efficient for 15 consecutive subcultures of the varieties analyzed.

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Section: Introductionmentioning

confidence: 99%

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Hsie¹,

Brito²,

Nova³

et al. 2015

Genet. Mol. Res.

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“…In later years, PAST has grown into a comprehensive statistics package that is used not only by paleontologists, but also in many fields of life science, earth science, and even engineering and economics (Hammer et al, 2001). PAST program was used in DNA fingerprinting (ISSR and RAPD) of Prosopis cineraria and P.juliflora (Elmeer and Almalki, 2011), molecular (SSR) analysis of old apple cultivars (Király et al, 2012), genetic analysis (RAPD) of Hibiscus species (Kadve et al, 2012), molecular (SSR) determination of genetic structure of Brazilian soybean cultivars (Piriolli et al, 2013), SSR-based genetic diversity assessment in tetraploid and hexaploid wheat populations (Abouzied et al, 2013), molecular diversity (SSR) in cultivated groundnut (Goswami et al, 2013). …”

Section: Resultsmentioning

confidence: 99%

Valencia ve Navel grup portakal çeşitlerinin SSR markörleri yardımıyla genetik çeşitlilik analizi

Polat¹

2015

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Sweet orange [Citrus sinensis (L.) Osbeck] fruit is one of the main citrus fruits, Navel and Valencia group sweet orange being the most representative and recognizable species of this species. The aims of this study were to determine genetic relationships and diversity of 84 Navel and 36 Valencia groups of sweet orange using SSR (simple sequence repeat) molecular markers. Twenty-six SSR primers were tested on these accessions. Seven SSR primers produced thirteen polymorphic fragments, eight SSR primers produced monomorphic fragments, and eleven SSR primers produced no scorable fragments. Thirteen SSR primers produced a total of 29 fragments and 13 of them were polymorphic. The number of average polymorphic fragments per primer was 1.93. The mean polymorphism information content (PIC) and marker index (MI) are 0.16 and 11.74, respectively. The Dice's similarity coefficient among Navel and Valencia group sweet oranges ranged from 0.42 to 1.00 and matrix correlation (r) was 0.79. In the cluster analysis, Navel group sweet oranges were indicated as a separate group from Valencia group sweet oranges. 'Antalya (40)' was most distinct accessions from the others.

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“…Several authors have reported the efficiency of using both types of markers (Ranade et al, 2009;Elmeer and Almalki, 2011;Mani et al, 2011;Sharma et al, 2010Sharma et al, , 2011.…”

Section: Discussionmentioning

confidence: 99%

“…Pharmawati et al (2004) evaluated the diversity among 16 genotypes of Grevillea using RAPD markers and found an average of 17.34 polymorphic bands per primer. Elmeer and Almalki (2011) assessed the genetic diversity of Prosopis cineraria and P. juliflora using RAPD markers and found 100% polymorphism. In this study, the PIC of the RAPD primers varied from 0.257 to 0.490, with an average of 0.364.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, polymerase chain reaction (PCR)-based molecular markers, such as those based on a single primer amplification reaction (SPAR), have increasingly become efficacious tools for studying genetic diversity in plants (Ranade et al, 2009;Elmeer and Almalki, 2011; J.D. Souza Neto et al Mani et al, 2011;Sharma et al, 2010Sharma et al, , 2011, particularly for those species that have not been well-studied or those for which specific molecular markers had not been developed.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of Anthurium genotypes by using DNA fingerprinting and SPAR markers

Neto¹,

Soares²,

Motta³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. We characterized single primer amplification reaction (SPAR) molecular markers from 20 genotypes of Anthurium andraeanum Lind., including 3 from commercial varieties and 17 from 2 communities in the State of Espírito Santo, Brazil. Twenty-four SPAR, 4767©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 4766-4775 (2014) Access to Anthurium genotypes diversity using SPAR markers consisting of 7 random amplified polymorphic DNA and 17 inter-simple sequence repeat markers were used to estimate the genetic diversity of 20 Anthurium accessions. The set of SPAR markers generated 288 bands and showed an average polymorphism percentage of 93.39%, ranging from 71.43 to 100%. The polymorphism information content (PIC) of the random amplified polymorphic DNA primers averaged 0.364 and ranged from 0.258 to 0.490. Primer OPF 06 showed the lowest PIC, while OPAM 14 was the highest. The average PIC of the inter-simple sequence repeat primers was 0.299, with values ranging from 0.196 to 0.401. Primer UBC 845 had the lowest PIC (0.196), while primer UCB 810 had the highest (0.401). By using the complement of Jaccard's similarity index and unweighted pair group method with arithmetic mean clustering, 5 clusters were formed with a cophenetic correlation coefficient of 0.8093, indicating an acceptable clustering consistency. However, no genotype clustering patterns agreed with the morphological data. The Anthurium genotypes investigated in this study are a germplasm source for conservational research and may be used in improvement programs for this species.

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DNA Finger Printing of <i>Prosopis cineraria</i> and <i>Prosopis juliflora</i> Using ISSR and RAPD Techniques

Abstract: Diversity within and among the populations of Prosopis cineraria and

Cited by 13 publications

References 41 publications

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Valencia ve Navel grup portakal çeşitlerinin SSR markörleri yardımıyla genetik çeşitlilik analizi

Molecular characterization of Anthurium genotypes by using DNA fingerprinting and SPAR markers

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