DNA Fingerprinting - An Asset to Forensic Dentistry

Sharma, Arpita; Sachdeva, Akshat; Bhateja, Sumit; Arora, Geetika; Giovannone, Riccardo; Petitti, Tommasangelo; Boyd, Stuart D.; Buscarini, Maurizio

doi:10.26717/bjstr.2018.09.001855

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…Also, tooth is most resistant to adverse conditions such as humidity, high temperature etc. without causing any damage to the DNA [7].…”

Section: Various Sources Of Dnamentioning

confidence: 98%

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DNA Profiling Advances in Forensic Dentistry- A Review

Shamala¹,

Beeula²,

S.³

et al. 2023

JAMMR

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Forensic Odontology is a branch of forensic science involves in human identification through dental tissues or more specifically teeth when there is the destruction of body tissues in criminal mass disasters. The importance of Forensic Dentistry in recent days lies mainly when there is little remaining material to perform such identification has led dentists working with forensic investigators to become more aware with the new molecular biology technologies. In the last few years, DNA analysis methods are more commonly applied in the forensic cases due to its uniqueness in identification of human remains even in the deceased state. DNA is a genetic material which is unique to each individual. In oral cavity and mainly in tooth structure DNA is found to be in abundant and hence it can be an excellent source of genomic DNA. Recently, DNA profiling has high reliability and is accepted as legal proofs in courts. This article mainly focuses on an overview of DNA and their applications in the last few years, highlighting its importance in forensic investigation.

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“…Also, tooth is most resistant to adverse conditions such as humidity, high temperature etc. without causing any damage to the DNA [7].…”

Section: Various Sources Of Dnamentioning

confidence: 98%

“…One of the main significance of PCR is that it is used to amplify the amount of DNA present. Amplification of the original DNA can be done, through standard Polymerase chain reaction which runs through 30 cycles for a couple of hours.DNA primers and special enzymes are used for the reaction [7,8,11].…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

DNA Profiling Advances in Forensic Dentistry- A Review

Shamala¹,

Beeula²,

S.³

et al. 2023

JAMMR

View full text Add to dashboard Cite

show abstract

“…The extraction of DNA method is divided into three phases: cell lysis (which enables the utilization of numerous methods for efficacious cellular membrane rupture), protein degradation and inhibition (by proteinases and chelating agents to deactivate components like protein molecules), and eventually DNA isolation on its own 9 .…”

Section: Dna Extractionmentioning

confidence: 99%

DNA Fingerprinting: A Revolution in the Field of Forensic Sciences

Pawar,

Gosavi,

Palaskar

et al. 2023

jfds

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DNA profiling, commonly called DNA fingerprinting or DNA testing, has a wide range of uses, including assisting in criminal cases and wildlife conservation. The firm tissues of the teeth are resistant to external events such as decomposition, bruise, and incineration. Pulp tissue provides a rich DNA source. The review focuses on the various DNA Collection Procedures, the different techniques employed in DNA isolation and amplification. There are a numerous DNA Profiling or Typing systems such as Mitochondrial DNA (mtDNA) analysis, Restriction Fragment Length Polymorphism, Y chromosome analysis, Single Nucleotide Polymorphism (SNP) typing , Short Tandem Repeat (STR) typing, Gender typing, X-chromosome STR Typing. DNA fingerprinting has been applied to solve many cases. Although present DNA analysis techniques are fairly specialized, several upcoming simplifications may increase their simplicity of use.

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Reliability of Automated Biometrics in the Analysis of Enamel Rod End Patterns- In Vitro Study

Singh,

Bhargava,

Khare

2023

Biosci., Biotech. Res. Asia

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Introduction: Ameloglyphics is the study of the distinctive patterns found on the enamel surface of teeth, which are different on each tooth and particular to each person. The purpose of this study was to compare the enamel print patterns on the buccal and lingual surfaces of teeth to see if there are any appreciable differences, and to evaluate the sensitivity and reliability of an automated biometrics software. Method: The study involved 62 individuals including 36 females and 26 males, from age range 15-60 years. From each individual one tooth sample was analyzed using a light microscope to capture images of the buccal and lingual surfaces middle third. These images were then analyzed using biometric software called Source AFIS, which processed the images and displayed enamel patterns. Data were entered and analyzed using GPower software (version 3.0). Chi-square test and Goodness of fit tests were done to compare the enamel rod end patterns between the teeth surfaces These enamel patterns were classified according to the “Manjunath Classification”. Result: The study found that there were no notable distinctions in enamel print patterns when comparing the teeth buccal and lingual surfaces. All 62 samples analyzed showed a high degree of similarity in enamel print patterns on both surfaces. This finding suggests that the same developmental, environmental, and genetic factors likely influence the enamel print patterns on both surfaces of a tooth. Conclusion: The samples' high frequency of comparable sub-patterns on both the buccal and lingual surfaces suggests that they are very similar to one another. This implies that tooth prints may be a valuable tool for forensic personal identification, particularly in the area of forensic dentistry.

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DNA Fingerprinting - An Asset to Forensic Dentistry

Abstract: After the evidence is collected from the crime scene, DNA is isolated. Using a special enzyme (restriction endonuclease) that acts as molecular scissors, DNA is cut into fragments at sites that are not found within the tandem repeat sequence. The chopped

Cited by 5 publications

References 23 publications

DNA Profiling Advances in Forensic Dentistry- A Review

DNA Profiling Advances in Forensic Dentistry- A Review

DNA Fingerprinting: A Revolution in the Field of Forensic Sciences

Reliability of Automated Biometrics in the Analysis of Enamel Rod End Patterns- In Vitro Study

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