DNA fingerprinting: an overview on genetic diversity studies in the botanical taxa of Indian Bamboo

Boban, Subin; Maurya, Shrinkhla; Jha, Zenu

doi:10.1007/s10722-021-01280-8

Cited by 3 publications

(1 citation statement)

References 86 publications

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“…Discovering the proper molecular markers for genetic fingerprinting is an important step in genetic estimation. For this explanation, different molecular markers are used and evaluated in the genetic fingerprinting of reasonably significant plant species (Dar et al 2019;Boban et al 2022;Zhang et al 2023). This also holds accuracy for Musa cultivars.…”

Section: Scot Polymorphism Analysismentioning

confidence: 99%

Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers

NITIWORAKARN,

PHAE-NGAM,

VANIJAJIVA

2023

Biodiversitas

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Abstract. Nitiworakarn S, Phae-Ngam W, Vanijajiva O. 2023. Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers. Biodiversitas 24: 4060-4068. Musa cultivars are mostly obtained from crossing between wild diploid subspecies of M. acuminata (A genome) and M. balbisiana (B genome), and they reveal numerous levels of ploidy and genomic constitution. The present study, for the first time, investigated the genetic diversity of 90 Musa cultivars from 17 localities of Chai Badan district in Lopburi Province, Thailand, using start codon targeted (SCoT) markers. The SCoT technique has shown very high repeatability for characterizing Musa cultivars. The result indicated that DNA fingerprints from 30 SCoT primers generated 244 amplicons, among which 238 bands (97.54%) were polymorphic, with a mean of 7.93 polymorphic bands per primer. The average polymorphism information content (PIC) was high, ranging from 0.345 to 0.483, with an average of 0.432. Genetic diversity and its partitioning parameters were calculated and demonstrated that present Musa cultivars maintain relatively high genetic diversity. The unweighted pair-group method with an arithmetic cluster analysis, principal coordinate analysis and STRUCTURE analysis results were the same and showed the clear division of the genotypes into two distinct clusters, which were cluster-I consisted of only the AA genomes and cluster-II comprised of ABB and BB genomes. In conclusion, SCoT markers obtained here showed their manipulation in genetic diversity and the relationship of cultivated Musa, which would be practical for genetic preservation and continuing breeding programs in Thailand and other regions.

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Section: Scot Polymorphism Analysismentioning

confidence: 99%

Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers

NITIWORAKARN,

PHAE-NGAM,

VANIJAJIVA

2023

Biodiversitas

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Association between molecular markers and resistance to bacterial blight using binary logistic analysis

Fatemifard,

Masoumiasl,

Rezaei

et al. 2024

BMC Plant Biol

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The most effective strategy for managing wheat bacterial blight caused by Pseudomonas syringae pv. syringae is believed to be the use of resistant cultivars. Researching the correlation between molecular markers and stress resistance can expedite the plant breeding process. The current study aims to evaluate the response of 27 bread wheat cultivars to bacterial blight disease in order to identify resistant and susceptible cultivars and to pinpoint ISSR molecular markers associated with bacterial blight resistance genes. ISSR markers are recommended for assessing a plant's disease resistance. This experiment is focused on identifying ISSR molecular markers linked to bacterial blight resistance. After applying the bacterial solution to the leaves, we performed sampling to determine the infection percentage in the leaves at different intervals (7, 14, and 18 days after spraying). In most cultivars, the average leaf infection percentage decreased 18 days after spraying on young leaves. However, in some cultivars such as Niknegad, Darab2, and Zarin, leaf infection increased in older leaves and reached up to 100% necrosis. In our study, 12 ISSR primers generated a total of 170 bands, with 156 being polymorphic. The primers F10 and F5 showed the highest polymorphism, while the F7 primer exhibited the lowest polymorphism. Cluster analysis grouped these cultivars into four categories. The resistant group included Qods, Omid, and Atrak cultivars, while the semi-resistant and susceptible groups comprised the rest of the cultivars. Through binary logistic analysis, we identified three Super oxide dismutase-related genes that contribute to plant resistance to bacterial blight. These genes were linked to the F3, F5, and F12 primers in regions I (1500 bp), T (1000 bp), and G (850 bp), respectively. We also identified seven susceptibility-associated genes. Atrak, Omid, and Qods cultivars exhibited resistance against bacterial blight, and three genes associated with this resistance were linked to the F3, F5, and F12 primers. These markers can be used for screening or transferring tolerance to other wheat cultivars in breeding programs.

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Deciphering the Ramet System of a Bamboo Plant in Response to Intensive Management

Gao

Zhizhuang

et al. 2022

Forests

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Intensive management is a common practice in bamboo plantations to ensure higher shoot yields. However, the effects of these management practices on ramet systems are understudied. A pot experiment was conducted to explore the process of propagation for potted bamboo seedlings (Phyllostachys praecox C. D. Chu et C. S. Chao “Prevernalis”) from a single mother bamboo to a ramet system exposed to a chronosequence of intensively managed bamboo forest soils. The ramet system of potted bamboo seedlings reached seven branching grades after two growth cycles. During ramet system expansion, the rhizome length and rhizome internodes decreased with increasing branching grade and the extension of intensive management periods. In the bud bank for each branching grade, the front branching grade was dominated by the bud output, which was conducive to consolidating the occupied living space. The back branching grade was dominated by bud input to continue rhizome penetration. With increasing branching, the mulching soil significantly inhibited rhizome bud germination and dormant bud accumulation. The mulching soil was not conducive to branch expansion in the ramet system, and ramet system expansion was predominantly based on the branching of the rhizome modules. With increasing branching and the extension of intensive management periods, rhizome branches decreased markedly. Our findings indicate that bamboo mulching inhibits branching and causes a differential reaction in branching types. The long-term mulching of bamboo forest soil was not conducive to the healthy and sustainable growth of bamboo. These results provide a basis for further research on the relationship between the bamboo ramet system and its productivity, as well as the population construction and maintenance mechanisms of bamboo ramet systems in the field.

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DNA fingerprinting: an overview on genetic diversity studies in the botanical taxa of Indian Bamboo

Cited by 3 publications

References 86 publications

Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers

Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers

Association between molecular markers and resistance to bacterial blight using binary logistic analysis

Deciphering the Ramet System of a Bamboo Plant in Response to Intensive Management

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