DNA fingerprinting and genetic diversity analysis with SCoT markers of Persian walnut populations (Juglans regia L.) in Iran

Tabasi, Melika; Sheidai, Masoud; Hassani, D.; Koohdar, Fahimeh

doi:10.1007/s10722-020-00914-7

Cited by 34 publications

(11 citation statements)

References 42 publications

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“…PCoA results in the current study also proved that there is a high level of genetic diversity in walnut accessions studied in this paper. Previous studies also indicated efficacy of PCoA used in walnut and in different plant species [46,48,[55][56][57][58][59][60][61][62][63][64].…”

Section: Genetic Relationships Among Walnut Accessionsmentioning

confidence: 99%

Genetic Diversity among Some Walnut (Juglans regia L.) Genotypes by SSR Markers

et al. 2021

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The food needs for increasing population, climatic changes, urbanization and industrialization, along with the destruction of forests, are the main challenges of modern life. Therefore, it is very important to evaluate plant genetic resources in order to cope with these problems. Therefore, in this study, a set of ninety-one walnut (Juglans regia L.) accessions from Central Anatolia region, composed of seventy-four accessions and eight commercial cultivars from Turkey, and nine international reference cultivars, was analyzed using 45 SSR (Simple Sequence Repeats) markers to reveal the genetic diversity. SSR analysis identified 390 alleles for 91 accessions. The number of alleles per locus ranged from 3 to 19 alleles with a mean value of 9 alleles per locus. Genetic dissimilarity coefficients ranged from 0.03 to 0.68. The highest number of alleles was obtained from CUJRA212 locus (Na = 19). The values of polymorphism information content (PIC) ranged from 0.42 (JRHR222528) to 0.86 (CUJRA212) with a mean PIC value of 0.68. Genetic distances were estimated according to the UPGMA (Unweighted Pair Group Method with Arithmetic Average), Principal Coordinates (PCoA), and the Structure-based clustering. The UPGMA and Structure clustering of the accessions depicted five major clusters supporting the PCoA results. The dendrogram revealed the similarities and dissimilarities among the accessions by identifying five major clusters. Based on this study, SSR analyses indicate that Yozgat province has an important genetic diversity pool and rich genetic variance of walnuts.

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Section: Genetic Relationships Among Walnut Accessionsmentioning

confidence: 99%

Genetic Diversity among Some Walnut (Juglans regia L.) Genotypes by SSR Markers

et al. 2021

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“…These markers represent a high level of polymorphism and efficiency. They are successfully utilized in different plant species such as Cicer species [ 17 ], bread wheat [ 18 ], date palm [ 18 ], and walnut [ 19 ]. ISSR markers are one of the most efficient marker systems because of their capacity to reveal several informative bands from a single amplification [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

Khodaee

Azizinezhad

Etminan

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Crop wild relatives (CWRs) are commonly used as a suitable genetic reservoir for plant breeding. They can be used for enhancing the tolerance of plant varieties to biotic and abiotic stresses. Studying the genetic diversity of related wheat species in Iran could be useful to improve different traits of bread wheat, since the country is one of the major centers of genetic diversity and distribution of Aegilops species. Therefore, the aim of the present study was to determine the relationship among 48 Aegilops triuncialis accessions using three DNA marker systems, including start codon targeted (SCoT), CAAT box-derived polymorphism (CBDP), and inter-simple sequence repeat (ISSR) markers. Results A total of 359 amplified DNA fragments were generated using 13 CBDP, 14 SCoT, and 16 ISSR primers that produced 96, 147, and 152 bands, respectively. The discriminating power of the three markers was assessed using polymorphism information content (PIC), marker index (MI), and resolving power (Rp). The mean values of PIC for ISSR, SCoT, and CBDP markers were 0.3, 0.26, and 0.34, respectively, indicating the efficiency of the three markers in detecting polymorphism among the studied accessions. ISSR markers had the highest values of MI, Rp, and polymorphism percentage as compared to SCoT and CBDP markers. Based on the Shannon index and heterozygosity values, genetic diversity in the Alborz population was more than in other populations. The accessions were classified into six, five, and five groups based on ISSR, SCoT, and CBDP using the UPGMA method. According to the results of cluster and PCoA analyses, the variation patterns corresponded with the geographical distribution of the Ae. triuncialis accessions. Conclusions The three markers provided a comprehensive pattern of the genetic diversity among the Iranian Ae. triuncialis accessions. This information could allow for a future insight into wheat breeding programs.

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“…Generally, genotypes gathered in the provinces of Midelt and Marrakech were divided into separate groups. Tabasi et al (2020a) showed similar results, where they showed that the populations collected from Semnan, Markazi, and Alborz provinces were almost divided into separate subsets. This is in agreement with Pollegioni et al (2014) who concluded that space and landscape feature act simultaneously on gene flow, influencing the genetic structure of the walnut populations.…”

Section: Genetic Similarity and Clustering Analysismentioning

confidence: 57%

“…In many places, walnut genotypes are known under different names so they can be the same. This is confirmed by Tabasi et al (2020a). Precise identification of walnut genotypes is an absolute requirement for good management and the use of genetic material in breeding and selection programs (Maghsoodi et al, 2018).…”

Section: Genetic Similarity and Clustering Analysismentioning

confidence: 78%

“…Precise identification of walnut genotypes is an absolute requirement for good management and the use of genetic material in breeding and selection programs (Maghsoodi et al, 2018). The obtained results showed that the ISSR markers are effective in identifying genotypes, assessing genetic diversity, and can be used for DNA fingerprinting of walnut populations (Tabasi et al, 2020a). This study will help map the associations in the walnut once the economic characteristics are associated with molecular discoveries.…”

Section: Genetic Similarity and Clustering Analysismentioning

confidence: 83%

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Molecular Diversity of Walnut (Juglans regia L.) Among Two Major Areas in Morocco in Contrast with Foreign Varieties

Houmanat

Kajji

Hssaini

et al. 2020

International Journal of Fruit Science

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In Morocco, walnut tree (Juglans regia L.) cultivation is ancestral with complicated misidentification and unknown local genetic diversity. Atlas Mountains host a rich walnut tree germplasm, which has arisen from seedlings. The use of molecular markers makes it possible to overcomes confusion usually encountered in phenotypic characterization, thereby elucidating the real genetic diversity magnitude. This study aimed to investigate the genetic relationships among 33 local walnut trees grown in two contrasted agroecosystems, while comparing them with 8 Bulgarian varieties belonging to an ex-situ collection, using ISSR markers. The use of 13 primers generated 120 reproducible ISSR markers, with 7 to 13 bands and an average of nine bands per primer. This number reflected the high level of polymorphism between genotypes revealed by the selected primers. The average polymorphism rate was 75.2%, with the polymorphic information content varying from 0.212 to 0.370 and with an average of 0.32. Genetic distances and the pairwise comparison showed that all pairs of genotypes are distinct using more than 10 markers with a maximum of 50 bands. The dendrogram showed an obvious phylo-geographic structuring and the differentiation of genotypes by origin area. The Bulgarian cultivars form distinct groups that confirms the specificity of the Moroccan walnut gene pool. However, the dendrogram obtained showed that the Moroccan genotypes are genetically close to a certain number of Bulgarian genotypes. This indicates that some Moroccan genotypes and the Bulgarian varieties could have a common ancestor.

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DNA fingerprinting and genetic diversity analysis with SCoT markers of Persian walnut populations (Juglans regia L.) in Iran

Cited by 34 publications

References 42 publications

Genetic Diversity among Some Walnut (Juglans regia L.) Genotypes by SSR Markers

Genetic Diversity among Some Walnut (Juglans regia L.) Genotypes by SSR Markers

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

Molecular Diversity of Walnut (Juglans regia L.) Among Two Major Areas in Morocco in Contrast with Foreign Varieties

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