DNA Fingerprinting and Quality Traits of Corbarino Cherry-like Tomato Landraces

Abstract

Twenty-five landraces of the cherry-like tomato named Corbarino, a typical niche product grown in the Sarno valley of the Campania region, have been characterized. The landraces used have been compared to eight cultivars widely spread in the same area of cultivation. The genetic diversity within and between landraces was evaluated through the comparison of DNA fingerprints obtained with (GATA)(4) probe hybridized to TaqI digested genomic DNA. Twenty-two of the Corbarino landraces were homogeneous and were unequivocally characterized by their DNA fingerprints. The others are probably "population varieties" in that within each of them polymorphic DNA fragments were identified. According to the characterization of fruit shapes, four groups were identified. One landrace from each group, harvested at the same ripening degree, was further characterized for yield, fruit quality components, antioxidant activities, and carotenoid contents and compared to Faino F(1) and Tomito F(1) hybrids. The Corbarino landraces were all characterized by high yield, a high level of carbohydrates, and high levels of soluble and total solids. Interestingly, the biotype named ISCI 05 was the most suitable for canning. Landraces ISCI 07 and ISCI 05 gave the highest value of antioxidant activities and carotenoid content. These data, together with previously published results, suggested that ISCI 05 should be proposed for the institution of a Label of Origin.

Chemotype and genotype combined analysis applied to tomato (Lycopersicon esculentum Mill.) analytical traceability

“…However, in practical production these factors are often variable and closely linked to one other (Hart and Scott, 1995). It is well known that the amount of each antioxidant in tomato fruits is strongly influenced by varietal differences (genetic influence), in addition to agronomical, geographical and environmental parameters (Hernandez et al, 2007;Abushita et al, 2000;Andrakis et al, 2004). Several hundred cultivars and hybrids of tomato having different chemical and nutritional characteristics are available on the market, in response to fresh consumption and industry requests.…”

Traditional Eastern Spanish varieties of tomato

“…Obviously, in these cases, the cause of the segregation observed should have emerged before the collection. Segregating or seed mixture populations have also been found in traditional tomato varieties in other areas, for example, in the "Corbarino" variety from Italy (Andreakis et al, 2004), though in that case, it remained unclear if that situation arose during the conservation process in the genebank or previously.…”

New genetic tools to identify and protect typical italian products

“…Nel pomodoro, sin dai primi studi effettuati mediante marcatori molecolari, fu chiaro che il livello di variabilità genetica era significativamente inferiore a quello riscontrato in altre specie ad auto-impollinazione e ciò è la conseguenza della stretta autogamia della specie oltre che della diffusa utilizzazione di materiali comuni in programmi di incrocio e selezione. Un marcatore dall'elevato potere discriminante è confermato essere il minisatellite (GATA) 4 , (Rao et al, 2006;Andreakis et al, 2004), marcatore altamente informativo, che evidenzia un profilo genetico identificativo di ogni varietà o accessione analizzata ed individua contaminazioni genetiche presenti in una stessa accessione (Caramante et al, 2009). Tra le altre classi di marcatori molecolari, gli SSR hanno mostrato elevati livelli di polimorfismo nella specie coltivata S. lycopersicum (Bredemeijer et al, 2002).…”