DNA fingerprinting differentiation between β-carotene hyperproducer strains of Dunaliella from around the world

Abstract

Background: Dunaliella salina is the most important species of the genus for β-carotene production. Several investigations have demonstrated that D. salina produces more than 10% dry weight of pigment and that the species grows in salt saturated lagoons. High plasticity in the green stage and the almost indistinguishable differences in the red phase make identification and differentiation of species and ecotypes very difficult and time consuming.

Apoptosis Comparison Effects Between Synthetic and Natural Β-Carotene from Dunaliella salina on MDA-MB-231Brest Cancer Cells

“…In this sense, Dunaliella isolates were morphological and molecularly identified. Figure 2, shown Dunaliella strain isolated from locations reported by Olmos and coworkers [11], picture presents a microalga growing on a brine lagoon containing high levels of β-carotene and two flagella, characteristics from a Dunaliella hyperproducer strain. Thus, Intron-Sizing-Method was applied to make an easy, fast and precise identification of the isolates [11,12].…”

“…Figure 2, shown Dunaliella strain isolated from locations reported by Olmos and coworkers [11], picture presents a microalga growing on a brine lagoon containing high levels of β-carotene and two flagella, characteristics from a Dunaliella hyperproducer strain. Thus, Intron-Sizing-Method was applied to make an easy, fast and precise identification of the isolates [11,12]. In natural samples "D. salina var Teod" was identified due its 18S rDNA fingerprinting profile presented a 2100 bp PCR product using MA1-MA2 conserved oligonucleotides and a 700 bp product by using D. salina specific and conserved oligonucleotides (DSs-MA2) (Figure 3).…”

“…Isolation of chromosomal DNA from Dunaliella strains was carried out and species identification of isolates was developed using MA1 [5'-CGGGATCCGTAGTCATATGCTTGTCTC-3'] and MA2 conserved and DSs (D. salina) [5'-GCAGGAGAGCTAATAGGA-3'] specific oligonucleotide reported by Olmos and coworkers [5,11]. MA1 and MA2 conserved oligonucleotides amplify 18S rDNA complete sequence.…”

“…MA1 and MA2 conserved oligonucleotides amplify 18S rDNA complete sequence. DSs oligonucleotide is specific for β-carotene "D. salina var Teod" hyper producer strain [11,12]. PCR reactions were carried out in a total volume of 100 μl containing 50 ng of chromosomal DNA in TE (Tris-EDTA) buffer, pH 8 and 200 ng MA1 and MA2 conserved primers.…”

“…D. salina and D. bardawil are the most important species of the genus for β-carotene production. Several investigations have demonstrated that both species produces more than 10% of its dry mass [11,12]. The commercial production of β-carotene that comes from Dunaliella is the third most important microalgae industry since 1986 [13].…”