DNA fingerprinting in roe deer using the digoxigenated probe (GTG)5

Mörsch, G.; Leibenguth, F.

doi:10.1111/j.1365-2052.1994.tb00443.x

Cited by 13 publications

(2 citation statements)

References 23 publications

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“…Postma et al (2001) using the same set of microsatellite loci as in our study on several introduced populations of roe deer in the Netherlands found a highly significant differentiation attributable to the divergent history of the populations and reduced migration over the highly urbanized landscape. Morsch and Leibenguth (1994) using neutral DNA markers, presented a similar conclusion based the absence of a significant differentiation between roe deer populations separated as far as 40 km apart by fenced highways and urban areas. In our study, the Nemunas and Neris rivers are ice free and could slow migration contributing to the genetic structure observed in our study.…”

Section: Geographic Location Effect On the Genetic And Morphometric Vsupporting

confidence: 60%

Differentiation of European roe deer populations and ecotypes in Lithuania based on DNA markers, cranium and antler morphometry

Sabalinkiene¹,

Danusevičius²,

Manton³

et al. 2017

Silva Fenn.

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Highlights• Lithuanian roe deer populations are genetically structured into southern and northern groups, most likely affected by a divergent gene flow and Lithuania's largest rivers slowing down migration.• Microsatellite and skull morphology based genetic differentiation between field and forest ecotypes are weak.• Geographical location has a significant effect on antler morphometry traits and skull size of male roe deer, the latter increasing northwards. AbstractThe objective of our study was to assess the genetic and morphological differentiation of European roe deer (Capreolus capreolus L.) in Lithuania based on DNA markers, skull and anther morphology. DNA was extracted from 79 culled individuals at 13 locations and genotyped at five nuclear microsatellite loci. Based on culling location, individuals were assigned to either a field (N = 43) or a forest ecotype (N = 36). Skull and antler morphometry was studied on 603 and 292 individuals, respectively. Results showed no significant genetic and skull morphology differentiation between the ecotypes. The forest ecotype tends to exhibit lower genetic diversity compared to the field ecotype, particularly for male individuals. The genetic differentiation of roe deer in Lithuania was significant based on the R ST values, but not on the F ST values. A STRUCTURE analyses revealed southern and northern genetic clusters, most likely affected by divergent gene flow. The country's major rivers Nemunas and Neris are likely to increase differentiation between the clusters. ANOVA on skull morphology by gender and age indicated a significant effect of geographical location. Skull size (especially length) is greater in the northern part of the country. We also found significant effects of age, ecotype and geographical location on most of the roe deer male antler morphometric traits.

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Section: Geographic Location Effect On the Genetic And Morphometric Vsupporting

confidence: 60%

Differentiation of European roe deer populations and ecotypes in Lithuania based on DNA markers, cranium and antler morphometry

Sabalinkiene¹,

Danusevičius²,

Manton³

et al. 2017

Silva Fenn.

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“…Figure 1 shows the fingerprints of 11 specimens of the Wustweiler subpopulation. Since somatic stability of the restriction fragment pattern was confirmed by preliminary experiments (Morsch & Leibenguth 1993), the organs from which DNA was isolated (spleen or kidney) are not specified. The following roe deer have a known degree of relatedness (r = 0.5): R5 is a 6-month-old female fawn of its mother R4 and R8 (9) and R 9 (6) are embryos of the female R10.…”

Section: Methodsmentioning

confidence: 99%

DNA fingerprinting in roe deer using the digoxigenated probe (GTG)5

Mörsch

Leibenguth

1994

Animal Genetics

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Summary The digoxigenin‐labelled oligonucleotide (GTG)5 was used as a multilocus probe to detect hypervariable microsatellites in roe deer DNA digested with Hae III. The resulting fingerprints of 24 animals belonging to four subpopulations were characterized with regard to within‐subpopulation as well as between‐subpopulation similarity. The mean number of polymorphic fragments was 20 and the average band‐sharing rate for unrelated animals 0.27. A mean probability of 91.5% for a fragment to be present in the heterozygous state was evaluated and the probabilities of identical band patterns in unrelated individuals were estimated to be in the range 1.3 times 10‐16 ‐ 2.5 times 10‐18. Though band‐sharing rates of animals belonging to different subpopulations (range 0.18‐0.24) were lower than those of within‐subpopulations, several measures of population subdivision and the genetic distance do not reveal a striking differentiation of the subpopulations studied.

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Informative enzyme/probe combinations for the multilocus DNA fingerprinting of marsupials

et al. 1997

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DNA fingerprinting has become an invaluable tool in the study of population genetics, paternity success, and individual identification; however, the species specificity of some methods has made the wide-range screening of many different species very time-consuming. In this study we describe the development and application of reliable and informative DNA fingerprinting techniques in a range of marsupial species using three different restriction enzyme and two oligonucleotide probe combinations. Six species from four marsupial families, the koala (Phascolarctidae), tammar wallaby (Macropodidae), southern hairy-nosed wombat (Vombatidae), kowari, and dusky and brown marsupial mice (Dasyuridae) were examined. Restriction enzymes HinfI AluI and HaeIII were used in combination with the digoxygenin (DIG)-labelled oligonucleotide probes (CAC)5 and (GGAT)4. The combinations of HinfI/(GGAT)4, AluI/ (CAC)5 and AluI/(GGAT)4 were the most informative, providing highly resolved bands, low background, and the lowest band sharing between individuals. The genetic diversity evident within the different species showed a clear relationship between the level of band sharing and population size. The greatest levels of band sharing were found in the kowaris (80%), which were part of a long-term captive colony originating from a few founders, and the lowest levels of band sharing were found in the marsupial mice (30-35%) and tammar wallaby (45%), which were caught from large outbred wild populations.

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DNA fingerprinting in roe deer using the digoxigenated probe (GTG)5

Cited by 13 publications

References 23 publications

Differentiation of European roe deer populations and ecotypes in Lithuania based on DNA markers, cranium and antler morphometry

Differentiation of European roe deer populations and ecotypes in Lithuania based on DNA markers, cranium and antler morphometry

DNA fingerprinting in roe deer using the digoxigenated probe (GTG)5

Informative enzyme/probe combinations for the multilocus DNA fingerprinting of marsupials

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