DNA FINGERPRINTING OF CHRYSANTHEMUM CULTIVARS USING RAPDs

Sehrawat, S. K.; Kumar, Ram; Dahiya, D. S.; Boora, K. S.; Yadav, R. K. S.

doi:10.17660/actahortic.2003.624.66

Cited by 9 publications

(4 citation statements)

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“…Ebrahimi et al (2010) also found that in most cases, the cluster results of 13 polymorphic loci were not correlated with the morphological traits of 13 pomegranate (P. granatum) cultivars. In the present study, all of the Japanese cultivars were clustered together in branch I; moreover, the Chinese-specific chrysanthemum germplasm, anemone, and dark red cultivars, closely clustered in branch II, indicating that the cultivars clustered according to the geographical origin, which is consistent with the results of Sehrawat et al (2003) and Shao et al (2010).…”

Section: Discussionsupporting

confidence: 89%

“…Martí et al (2002) found that 15 commercial chrysanthemum cultivars could be distinguished from each other through RAPD analysis, and the level of similarity between the cultivars was low. Sehrawat et al (2003) classified 13 chrysanthemum cultivars into two primary groups using RAPD markers and found that the results were consistent with the morphological differences and geographical distributions. In another study, the molecular characterizations of 10 new radiomutants of chrysanthemum were analyzed (Lema-Rumiñska et al, 2004).…”

Section: Discussionmentioning

confidence: 53%

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Assessing the Genetic Diversity of Chrysanthemum Cultivars with Microsatellites

Zhang

Wang²,

Ma³

et al. 2013

J. Amer. Soc. Hort. Sci.

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The morphological characteristics of chrysanthemum (Chrysanthemum ×morifolium) are rich in variation. However, as a result of the aneuploid polyploidy of the chrysanthemum genome and the lack of proper tools, the genomic information of this crop is limited. Development of microsatellite markers has been an increasing trend in crop genetic studies because of the applicability of these markers in breeding programs. In this study, we reported the development of a simple sequence repeat in chrysanthemums using a magnetic beads enrichment method. An enriched genomic library with AC and GT microsatellite motifs was constructed, and 53 positive clones were detected by a colony polymerase chain reaction (PCR) technique. Of these clones, 35 showed high-quality sequences, and 35 primer pairs were designed accordingly. Twenty-six (74.29%) of the 35 primer pairs revealed polymorphisms on a set of 40 chrysanthemum cultivars. There were 172 alleles amplified over 26 loci with an average of 6.615 alleles per locus. The mean values of gene diversity corrected for the sample size and the inbreeding coefficient were 0.609 and 0.119 over 26 loci, respectively, which indicated that the majority of the microsatellite loci is highly informative. Cluster analysis based on 26 polymorphic loci demonstrated that the selected cultivars were clustered according to geographical origin. This study shows the isolation efficiency of the magnetic beads technique; the abundance of microsatellites in chrysanthemum; and the potential application for the cultivar classification, the studies on genetic diversity, and molecular breeding of chrysanthemums, which is beneficial to promoting the conservation and sustainable use of this crop.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 53%

Assessing the Genetic Diversity of Chrysanthemum Cultivars with Microsatellites

Zhang

Wang²,

Ma³

et al. 2013

J. Amer. Soc. Hort. Sci.

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“…There are many methods that have been used for the classification of chrysanthemums. Previous studies on the classification of chrysanthemums have mainly focused on the quantitative taxonomy (Du, ; Liu & Wang, ), cytotaxonomy (Zhang et al, ), isoenzyme classification research (Roxas et al, ), and molecular marker research (Sehrawat et al, ; Chatterjee et al, ). For example, Liu & Wang () carried out a numerical classification study on 78 chrysanthemum cultivars by analyzing 38 characters, and pointed out that the first and second classification criteria for chrysanthemums were the petal type and the flower head type, which was consistent with the present study.…”

Section: Discussionmentioning

confidence: 99%

A classification study for chrysanthemum (Chrysanthemum ×grandiflorum Tzvelv.) cultivars based on multivariate statistical analyses

Zhang

Luo

Zhu

et al. 2014

J of Sytematics Evolution

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The Chinese traditional chrysanthemum cultivar is a marvelous group of Chrysanthemum ×grandiflorum Tzvelv., whose floral characters are rich in variation. However, neither the values of floral characters for the classification of chrysanthemums nor the relevance of different characters are yet known. Moreover, the existing classification systems of chrysanthemums are incomplete and unsystematic. In the present study, we selected 16 floral morphological characters on which to carry out diversity and relativity analyses by seven multivariate statistical analysis methods, and we further studied the cultivar classification. The analyses of nested variance, diversity index, and principle components showed that 10 floral morphological characters were of great value for chrysanthemum classification. Clustering analysis classified these 10 characters into three types, in which the variation of the ray floret factor was the highest. The analysis of partial correlations showed that the petal type was significantly affected by the length of the ray florets, the bending state of the outer‐layer ray florets, and the tip shape of the ray florets; besides these three characters, the flower head type was also affected by the angle of the outer‐layer ray florets and the length and distribution of the disc florets. The multiple linear regression equations for petal type and flower head type were successfully established. All of the cultivars were classified into four groups and 18 forms by cluster and discriminant analyses. These results provide a reference that can be used for cultivar identification and classification of chrysanthemums.

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“…Especially, cultivars possess similar morphological features can be distinguished by molecular markers very well. In recent years, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), inter-simple sequence repeats (ISSR), simple sequence repeats (SSR) and sequence-related amplified polymorphism (SRAP) markers have been used to identify sports or classify cultivars in chrysanthemum [14] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] . Among these markers, SSR markers have gained considerable popularity due to their many desirable attributes, including hypervariability, a multiallelic nature, codominant inheritance, reproducibility, relative abundance, extensive genomic coverage (including organellar genomes), chromosome-specific location, amenability to automation, and high-throughput genotyping [28] .…”

Section: Introductionmentioning

confidence: 99%

Application of Genomic SSR Locus Polymorphisms on the Identification and Classification of Chrysanthemum Cultivars in China

et al. 2014

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The Chinese traditional chrysanthemum is a notable group of chrysanthemums (Chrysanthemum×morifolium Ramat.) in which the phenotypic characteristics richly vary. At present, there is a serious controversy regarding homonyms and synonyms within this group. Moreover, the current international chrysanthemum classification systems are not comprehensive enough to be used on Chinese traditional chrysanthemums. Thus, we first identified a broad collection of 480 Chinese traditional chrysanthemum cultivars using the unique DNA fingerprints and molecular identities that were established by 20 simple sequence repeat markers. Five loci, which distinguished all of the selected cultivars, were identified as the core loci to establish unique fingerprints and molecular identities with 19 denary digits for each cultivar. A cluster analysis based on Nei's genetic distance indicated that the selected cultivars were clustered according to their horticultural classification. Population structure analysis was subsequently performed with K values ranging from 2 to 14, and the most likely estimate for the population structure was ten subpopulations, which was nearly consistent with the clustering result. Principal component analysis was further performed to verify the classification results. On the basis of the Q-matrices of K = 10, a total of 19 traits were found to be associated with 42 markers. Taken together, these results can serve as starting points for the identification and classification of chrysanthemums based on the polymorphism of microsatellite markers, which is beneficial to promote the marker-assisted breeding and international communication of this marvelous crop.

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DNA FINGERPRINTING OF CHRYSANTHEMUM CULTIVARS USING RAPDs

Cited by 9 publications

References 0 publications

Assessing the Genetic Diversity of Chrysanthemum Cultivars with Microsatellites

Assessing the Genetic Diversity of Chrysanthemum Cultivars with Microsatellites

A classification study for chrysanthemum (Chrysanthemum ×grandiflorum Tzvelv.) cultivars based on multivariate statistical analyses

Application of Genomic SSR Locus Polymorphisms on the Identification and Classification of Chrysanthemum Cultivars in China

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