DNA fingerprinting of Flavobacterium columnare using RAPD-PCR

Abstract

In the present study, DNA fi ngerprinting of eight strains of Flavobacterium columnare was done by random amplifi cation of polymorphic DNA (RAPD) fi ngerprinting method. The strains were collected from Fish Health Management Division, Central Institute of Freshwater Aquaculture, Bhubaneswar, India. A total number of 160 primers were screened for RAPD-PCR, of which 10 primers yielded amplifi cation with all the strains. The molecular weight of amplifi ed bands varied from 0.29-2.63 Kb. The number of bands varied from 1 to 8. Unique band was seen with primer OPY-15 with molecular weight 0.75 Kb that can be used for epidemiological study. Genetic variability was investigated using NTSYS software. Highest genetic similarity was found between MS1 and MS3 followed by MS5 and MS7. Minimum genetic similarity was found between MS2 and MS8. Phylogenetic tree was constructed using UPGMA and neighbor joining methods.

Molecular Identification and Characterization of Different Fusarium oxysporum f.sp. lycopersici Isolates Using RPAD-PCR Marker

“…Genetic diversity for each primer was calculated as previously described (Owen et al, 1998) using the following formula (H = 1-Σxi 2 ), where H is the expected heterozygosity and xi is the frequency of allele i, including "nulls" as a separate band. It works on the assumption that the rates of evolution in all lineages are same and gives output clustering in increasing order of distance (Sahoo et al 2010).…”