DNA fingerprinting of medically important microorganisms by use of PCR

Belkum, Alex van

doi:10.1128/cmr.7.2.174

Cited by 257 publications

(186 citation statements)

References 121 publications

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“…Ribotyping [19] and RAPD-PCR [20,21] have been used for detecting polymorphism within medically important organisms. However, the value of any typing procedure depends on the discriminatory power of the particular technique being applied.…”

Section: Discussionmentioning

confidence: 99%

Subtype distribution of Haemophilus influenzae isolates from North India

Sharma

Kaur

Ganguly

et al. 2002

Journal of Medical Microbiology

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A total of 120 Haemophilus influenzae isolates from blood, cerebrospinal fluid, sputum and throat swabs of patients and carriers in North India was characterised by biotyping, ribotyping and random amplification of polymorphic DNA (RAPD)-PCR. Of these, 77 isolates (64%) were serotype b; the other 43 (36%) were non-typable. Biotype I was the most predominant among the typable strains and biotype II among the non-typable strains. Ribotyping with restriction endonucleases HaeIII and EcoRI differentiated the isolates into three and six ribotypes, respectively. However, RAPD fingerprints generated by the application of arbitrary primers AP1 and AP2 provided a higher level of discrimination. RAPD typing revealed distinct polymorphism among the serologically typable isolates. This study is the first report that stratifies the subtypes of H. influenzae strains from India by molecular techniques.

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Section: Discussionmentioning

confidence: 99%

Subtype distribution of Haemophilus influenzae isolates from North India

Sharma

Kaur

Ganguly

et al. 2002

Journal of Medical Microbiology

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“…In contrast to traditional target-specific PCR, no prior sequence information is required and the technique is potentially applicable to all bacteria. This technique has been applied successfully to epidemiological investigations of many bacterial genera and species [20]. In this report, the application of this random amplification of polymorphic DNA (RAPD) method to investigate the epidemiological relationship of isolates of E. aerogenes obtained from patients from two intensive care units is described.…”

Section: Introductionmentioning

confidence: 99%

Investigation of outbreaks of Enterobacter aerogenes colonisation and infection in intensive care units by random amplification of polymorphic DNA

Davin-Régli¹,

Saux²,

Bollet³

et al. 1996

Journal of Medical Microbiology

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During a 4-month period, 41 isolates of Enferobacfer aerogenes were cultured from different specimens from a 14-bed intensive care unit (ICUl). These were obtained from 12 patients out of a total of 187 patients admitted to the ICU. Sixteen E. aerogenes isolates were cultured from another ICU (ICU2) 6 months later. Six non-outbreakassociated strains were included as controls and all the isolates were compared by random amplification of polymorphic DNA (RAPD), with three different 10-mer oligonucleotide primers. The six non-outbreak-associated strains were distinguishable by RAPD with two of the three primers. RAPD fingerprinting with primer AP12h was as discriminatory as the combined results from all three primers and defined 22 different patterns for the 41 isolates from the ICU1. In nine instances, isolates with indistinguishable RAPD patterns were detected in two-to-five patients over a 3-15-day period, suggesting patient-to-patient transmission. During their stay in ICU1, patients harboured one-to-12 distinguishable isolates. Isolates from ICU2 were indistinguishable by RAPD analysis with the three different primers. These findings suggest that the cluster of colonisations and infections in ICUl was a 'false outbreak', consisting of successive patient-to-patient transmission of different E. aerogenes strains. In contrast, the outbreak on ICU2 probably involved the extensive spread of a single strain.

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“…Typing of enterococci has been accomplished by protein analysis, biochemical profiles and antibiotic susceptibility (8,9). However, the lack of discriminatory power of such techniques has led researchers to develop alternative molecular-based methods (10,11).…”

Section: Introductionmentioning

confidence: 99%

Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis

Bedendo

Pignatari

2000

Braz J Med Biol Res

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Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eight Enterococcus faecium isolates from Stanford University and 12 from São Paulo Hospital were studied by JB1-PCR, REP-PCR 1/2R and PFGE. Among the isolates from Stanford University, 5 genotypes were defined by JB1-PCR, 7 by REP-PCR 1/2R and 4 by PFGE. Among the isolates from São Paulo Hospital, 9 genotypes were identified by JB1-PCR, 6 by REP-PCR and 5 by PFGE. The three methods identified identical genotypes, but there was not complete agreement among them.

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DNA fingerprinting of medically important microorganisms by use of PCR

Cited by 257 publications

References 121 publications

Subtype distribution of Haemophilus influenzae isolates from North India

Subtype distribution of Haemophilus influenzae isolates from North India

Investigation of outbreaks of Enterobacter aerogenes colonisation and infection in intensive care units by random amplification of polymorphic DNA

Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis

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