DNA fingerprinting of Paenibacillus popilliae and Paenibacillus lentimorbus using PCR-amplified 16S–23S rDNA intergenic transcribed spacer (ITS) regions

Paenibacillus larvae 16S–23S rDNA intergenic transcribed spacer (ITS) regions: DNA fingerprinting and characterization

“…Uniqueness is supported by a previous report in which P. larvae NRRL B-3555, one of 11 species tested, exhibited the six-band profile in relation to profiles of other Paenibacillus species (Dingman, 2009).…”

“…ITS-PCR fingerprint patterns of P. larvae strains An ITS-PCR profile for P. larvae strain NRRL B-3555 was previously reported as part of an investigation to define ITS-PCR fingerprint patterns of P. popilliae and P. lentimorbus (Dingman, 2009). To determine if this pattern was common for P. larvae and, thus, would serve as a DNA fingerprinting pattern for identification (i.e., ITS-PCR fingerprinting), numerous isolates of P. larvae, obtained from honey bees collected at different sites within Connecticut and from isolates collected at different geographical locations (Table 1), were tested using PCR-amplification of the ITS regions.…”

“…Jensen and associates (1993) used ITS-PCR fingerprinting to identify over 300 strains of bacteria comprising 28 species and eight genera. ITS-PCR DNA fingerprinting has been applied to the identification of Paenibacillus popilliae/Paenibacillus lentimorbus and has been suggested as useful for identification of other paenibacilli (Dingman, 2009).…”

Survey of pathogens and parasitoids of Popillia japonica (Coleoptera: Scarabaeidae) in northwest Arkansas

“…The most commonly reported have been milky disease bacteria Paenibacillus spp. (Dingman, 2009;Hutton and Burbutis, 1974;Cappaert and Smitley, 2002), the microsporidian Ovavesicula popilliae (Andreadis and Hanula, 1987), the gut protist Stictospora villani (Hays et al, 2004), a tiphiid parasitoid, Tiphia vernalis Rohwer (Rogers and Potter, 2004;Ramoutar and Legrand, 2007), and the tachinid fly, Istocheta aldrichi Mesnil (Fleming, 1968). The prevalence of these natural enemies in the Arkansas beetle population has been unknown.…”