DNA Flow-Cytometric Analysis of Basal Cell Carcinomas and Its Relevance to Their Morphological Differentiation: A Retrospective Study

Fortier-Beaulieu, M; Laquerrière, Annie; Thomine, E; Lauret, P; Hémet, J

doi:10.1159/000247109

Cited by 9 publications

(6 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The incidence of DNA aneuploidy in BCC is much lower (9%-40%) than observed for SCC (Frentz and Moller, 1983;Robinson et al, 1996;Staibano et al, 2001). However, different subgroups of BCC, such as keratotic and metatypical subgroups, have been shown to have much higher aneuploidy rates (Fortier-Beaulieu et al, 1994). Furthermore, Staibano and colleagues (2001) suggested that poor clinical outcome from recurrent/ metastatic hyperpigmented BCCs could be predicted on the basis of aneuploidy and cyclin D1 expression.…”

Section: Dna Ploidy Of Nmscmentioning

confidence: 99%

Cytogenetic alterations in nonmelanoma skin cancer: A review

Ashton

Carless

Griffiths

2005

Genes Chromosomes & Cancer

View full text Add to dashboard Cite

Since the advent of cytogenetic analysis, knowledge about fundamental aspects of cancer biology has increased, allowing the processes of cancer development and progression to be more fully understood and appreciated. Classical cytogenetic analysis of solid tumors had been considered difficult, but new advances in culturing techniques and the addition of new cytogenetic technologies have enabled a more comprehensive analysis of chromosomal aberrations associated with solid tumors. Our purpose in this review is to discuss the cytogenetic findings on a number of nonmelanoma skin cancers, including squamous- and basal cell carcinomas, keratoacanthoma, squamous cell carcinoma in situ (Bowen's disease), and solar keratosis. Through classical cytogenetic techniques, as well as fluorescence-based techniques such as fluorescence in situ hybridization and comparative genomic hybridization, numerous chromosomal alterations have been identified. These aberrations may aid in further defining the stages and classifications of nonmelanoma skin cancer and also may implicate chromosomal regions involved in progression and metastatic potential. This information, along with the development of newer technologies (including laser capture microdissection and comparative genomic hybridization arrays) that allow for more refined analysis, will continue to increase our knowledge about the role of chromosomal events at all stages of cancer development and progression and, more specifically, about how they are associated with nonmelanoma skin cancer.

show abstract

Section: Dna Ploidy Of Nmscmentioning

confidence: 99%

Cytogenetic alterations in nonmelanoma skin cancer: A review

Ashton

Carless

Griffiths

2005

Genes Chromosomes & Cancer

View full text Add to dashboard Cite

show abstract

“…Whether this is the explanation for their low metastatic potential remains to be seen . There have been few studies on aneuploidy rates in BCC using cytometry, as well as using classical and molecular cytogenetic methods . Yet, there is no study that would genetically characterize different histopathologic subtypes of BCC.…”

mentioning

confidence: 99%

Basal cell carcinoma of the skin: whole genome screening by comparative genome hybridization revisited

et al. 2012

View full text Add to dashboard Cite

Basal cell carcinoma (BCC) of the skin is considered to be the most common malignancy in people of European ancestry. It is often not clinically aggressive and has been regarded as genetically stable. However, histopathologic subtypes of BCC differ in their ability to invade surrounding tissues and recur. The aim of this work was to present a comprehensive study of chromosomal imbalances of cutaneous BCC and to correlate the findings with their histopathologic and clinical features. In all, 101 tumor samples were classified according to the current microscopic classification of BCC and then analyzed by comparative genomic hybridization (CGH). Over 60% of BCC were found to carry chromosomal imbalances - partial or whole chromosome gains and losses. Different subtypes of BCC presented common chromosomal alterations. No single chromosomal imbalance was found to be characteristic of a particular subtype of BCC, although the frequency of some chromosomal changes differed from one group to the other. The significance of chromosome 2 gains as a phenomenon that does not coexist with the losses in 9q is discussed.

show abstract

“…We observed allelic loss of a novel cancer‐associated gene, NAV3 , in 20–25% of BCC and SCC tumors and showed that silencing of the NAV3 gene up‐regulates a number of genes relevant for tumor cell growth in primary human keratinocytes. BCC is known to show a high frequency of non‐clonal, structural chromosomal rearrangements and an increased frequency of DNA aneuploidy, especially in BCC exhibiting infiltrative and superficial multicentric growth patterns (23) and metatypical differentiation (24). Likewise, aneuploidy has been found to associate with local metastasis in SCC as well as in head and neck squamous cell carcinoma (25,26).…”

Section: Discussionmentioning

confidence: 99%

“…To obtain a better insight into the effect of NAV3 silencing, we analysed several post-silencing time points separately, because the point of maximal silencing is not necessarily the optimal time point for monitoring the expression of potential target genes. Post-transfection RNA samples from four different time points (6,24,48 and 72 h) were extracted with RNeasy Micro or Mini Kit (Qiagen, Hilden, Germany) and stored at )70°C. Efficient NAV3 silencing was confirmed by quantitative PCR and Western blot ( Fig.…”

Section: Nav3 Gene Silencing In Vitromentioning

confidence: 99%

NAV3 copy number changes and target genes in basal and squamous cell cancers

Maliniemi¹,

Carlsson²,

Kaukola³

et al. 2011

Experimental Dermatology

View full text Add to dashboard Cite

The neuron navigator 3 (NAV3) gene on chromosome 12q21 encodes a microtubule plus end tracking protein and belongs to the navigator family of cytoskeletal regulators. Loss of heterozygosity on 12q has previously been suggested to be associated with poor prognosis in cancers of epithelial origin. In this study, we characterized copy number changes of NAV3 in 24 basal cell cancers (BCCs), eight squamous cell cancers (SCCs) and eight non-malignant inflammatory skin lesions by fluorescent in situ hybridization. To identify genes affected by NAV3, we used oligo siRNA gene silencing and gene microarrays to analyse gene expression profiles at several time points post-transfection in primary human keratinocytes. We found NAV3 copy number loss and decreased protein expression in 21% of the BCCs and 25% of the SCCs. In the nodular/superficial BCC subgroup, low-level NAV3 amplification was also observed. NAV3 aberrations were independent of the known chromosome 6 amplifications in BCC. Chromosome 12 polysomy was detected in 33% and 25% of the invasive type of BCC and SCC, respectively. Silencing of NAV3 in primary human keratinocytes revealed 22 differentially expressed genes, mostly related to inflammation. The most relevant of these were validated with qPCR or immunohistochemistry. This pilot study suggests that NAV3 is a novel cancer-associated gene that contributes to the pathogenesis of a subgroup of BCC and SCC.

show abstract

DNA Flow-Cytometric Analysis of Basal Cell Carcinomas and Its Relevance to Their Morphological Differentiation: A Retrospective Study

Cited by 9 publications

References 11 publications

Cytogenetic alterations in nonmelanoma skin cancer: A review

Cytogenetic alterations in nonmelanoma skin cancer: A review

Basal cell carcinoma of the skin: whole genome screening by comparative genome hybridization revisited

NAV3 copy number changes and target genes in basal and squamous cell cancers

Contact Info

Product

Resources

About