DNA forms indicate rolling circle and recombination-dependent replication of Abutilon mosaic virus

Jeske, Holger

doi:10.1093/emboj/20.21.6158

Cited by 228 publications

(197 citation statements)

References 48 publications

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“…1b). As reported previously (Jeske et al, 2001), these signal smears bury the secrets of replicational dynamics, which can be unravelled by 2D electrophoresis (Figs 2 and 3). …”

supporting

confidence: 72%

“…All of the MSV 2D blots showed clear evidence of RDR intermediates, indicated by faint arcs originating on dimeric cccDNA spots (labelled RDR in Figs 2 and 3). As described elsewhere (Jeske et al, 2001), similar intermediates originating on monomeric cccDNA spots could not be resolved in our 2D gels, because they are overprinted by the prominent tracks formed by the hdsDNA molecules that are produced as a result of RDR.…”

mentioning

confidence: 94%

“…In begomoviruses and curtoviruses, recombination-dependent replication (RDR) has been identified as a third replication mode that utilizes viral covalently closed circular DNA (cccDNA) as template molecules (Alberter et al, 2005;Jeske et al, 2001;Jovel et al, 2007;Morilla et al, 2006;Pilartz & Jeske, 2003;Preiss & Jeske, 2003). Mastreviruses might behave differently from members of the other genera in the family because they are the only geminiviruses to express RepA.…”

mentioning

confidence: 99%

“…Furthermore, they lack genes encoding transcriptional activator and replicational enhancer proteins that are found in begomoviruses and curtoviruses. Although neither of these proteins is absolutely required for RDR (Jeske et al, 2001), the possibility remains that they may enhance the efficiency of the process.…”

mentioning

confidence: 99%

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Replicative intermediates of maize streak virus found during leaf development

Erdmann

Shepherd

Martin

et al. 2009

Journal of General Virology

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Geminiviruses of the genera Begomovirus and Curtovirus utilize three replication modes: complementary-strand replication (CSR), rolling-circle replication (RCR) and recombinationdependent replication (RDR). Using two-dimensional gel electrophoresis, we now show for the first time that maize streak virus (MSV), the type member of the most divergent geminivirus genus, Mastrevirus, does the same. Although mastreviruses have fewer regulatory genes than other geminiviruses and uniquely express their replication-associated protein (Rep) from a spliced transcript, the replicative intermediates of CSR, RCR and RDR could be detected unequivocally within infected maize tissues. All replicative intermediates accumulated early and, to varying degrees, were already present in the shoot apex and leaves at different maturation stages. Relative to other replicative intermediates, those associated with RCR increased in prevalence during leaf maturation. Interestingly, in addition to RCR-associated DNA forms seen in other geminiviruses, MSV also apparently uses dimeric open circular DNA as a template for RCR.

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“…1b). As reported previously (Jeske et al, 2001), these signal smears bury the secrets of replicational dynamics, which can be unravelled by 2D electrophoresis (Figs 2 and 3). …”

supporting

confidence: 72%

mentioning

confidence: 94%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Replicative intermediates of maize streak virus found during leaf development

Erdmann

Shepherd

Martin

et al. 2009

Journal of General Virology

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“…In particular, a coupling between viral replication and recombination has been established for geminivirus (abutilon mosaic virus) infecting plant cells and agroinfection (Jeske et al, 2001). The authors concluded that the replication-dependent recombination (RDR) might indeed be a general replication process in this virus group.…”

Section: Introductionmentioning

confidence: 99%

T‐DNA recombination and replication in maize cells

Zhao

Coats

et al. 2003

The Plant Journal

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SummaryT-DNA recombination and replication was analyzed in 'black mexican sweet' (BMS) cells transformed with T-DNAs containing the replication system from wheat dwarf virus (WDV). Upon recombination between the T-DNA ends, a promoterless marker gene (gusA) was activated. Activation of the recombination marker gene was delayed and increased exponentially over time, suggesting that recombination and amplification of the T-DNA occurred in maize cells. Mutant versions of the viral initiator gene (rep), known to be defective in the replication function, failed to generate recoverable recombinant T-DNA molecules. Circularization of T-DNA by the FLP/FRT site-specific recombination system and/or homologous recombination was not necessary to recover circular T-DNAs. However, replicating T-DNAs appeared to be suitable substrates for site-specific and homologous recombination. Among 33 T-DNA border junctions sequenced, only one pair of identical junction sites was found implying that the population of circular T-DNAs was highly heterogenous. Since no circular T-DNA molecules were detected in treatments without rep, it suggested that T-DNA recombination was linked to replication and might have been stimulated by this process. The border junctions observed in recombinant T-DNA molecules were indicative of illegitimate recombination and were similar to left-border recombination of T-DNA into the genome after Agro-mediated plant transformation. However, recombination between T-DNA molecules differed from T-DNA/genomic DNA junction sites in that few intact right borders were observed. The replicating T-DNA molecules did not enhance genomic random integration of T-DNA in the experimental configuration used for this study.

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Geminiviridae

Briddon¹,

Stanley²

2009

Encyclopedia of Life Sciences

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The Geminiviridae comprise a group of plant viruses with small, circular, single‐stranded deoxyribonucleic acid (DNA) genomes encapsidated in particles that have a twinned quasi‐icosahedral (geminate) shape, from which the family derives its name. They are among the smallest of viruses known and are transmitted exclusively by specific insect vectors. Some are associated with highly diverse circular, single‐stranded DNA betasatellites that are essential for disease progression. Their association with alphasatellites provides evidence of the interaction of geminiviruses with other families of plant‐infecting viruses. Collectively, geminiviruses are pathogens of cereals, vegetable and fibre crops, posing a serious threat to agriculture worldwide. Efforts to control these viruses by conventional means, such as using insecticides against their insect vectors and by breeding resistance, have either proven unsuccessful or have not been durable. For this reason, researchers are increasingly looking to engineered resistance as a means to control these important diseases. Key Concepts: Geminiviruses are important insect‐transmitted pathogens of cereal, vegetable and fibre crops in tropical and temperate regions throughout the World, including developed and developing countries. Geminiviruses often cause striking symptoms in plants, indeed the earliest description of virus symptoms in plants is believed to relate to those caused by a geminivirus, and such symptoms occasionally are considered to be horticulturally desirable. All geminiviruses have single‐stranded DNA genomes and a twinned particle morphology that is unique to this family of plant viruses. Some geminiviruses are associated with single‐stranded DNA satellites, referred to as alphasatellites and betasatellites, the latter playing an essential role in maintenance of the disease. Because their small DNA genomes encode only a limited number of genes, geminiviruses manipulate the metabolism of the infected cell to produce conditions suitable for their proliferation. Geminiviruses are proving invaluable in the investigation of fundamental biological processes such as DNA replication, cell cycle regulation, gene expression and pathogen resistance in plants. Geminiviruses are highly recombinogenic, and the derivation of a large number of geminivirus and satellite DNA sequences is providing important insights into their diversity, adaptability and evolution. Conventional control strategies, such as the use of chemicals to eliminate their insect vectors and breeding natural host plant resistance, have proven ineffective in reducing crop losses to geminivirus infection, leading to the development of promising alternative (genetically engineered) strategies. Sequences derived from geminiviruses are being used to develop systems for high‐level expression of valuable proteins in plants.

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DNA forms indicate rolling circle and recombination-dependent replication of Abutilon mosaic virus

Cited by 228 publications

References 48 publications

Replicative intermediates of maize streak virus found during leaf development

Replicative intermediates of maize streak virus found during leaf development

T‐DNA recombination and replication in maize cells

Geminiviridae

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