DNA four-way junction-driven dual-rolling circle amplification sandwich-type aptasensor for ultra-sensitive and specific detection of tumor-derived exosomes

Zhao, Zhuyang; Yang, Sha; Tang, Xiaoqi; Feng, Liu; Ding, Zishan; Chen, Zhiguo; Luo, Xing; Deng, Ruijia; Sheng, Jing; Xie, Shuang; Chang, Kai; Chen, Ming

doi:10.1016/j.bios.2023.115841

Cited by 12 publications

(2 citation statements)

References 70 publications

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“…Electrochemical biosensing is a low-cost, easy-to-use, minimally affected by a low background signal, highly sensitive, and fast real-time system, thereby garnering considerable interest from researchers[ 39 ]. The utilization of aptamer recognition and signal amplification techniques augments the electrochemical signal, thereby bolstering detection sensitivity and specificity[ 40 ]. Thus, it exhibits broad application prospects in the field of tumor diagnosis and therapy.…”

Section: Discussionmentioning

confidence: 99%

Trends and frontiers in signal amplification for aptamer-based tumor detection: A bibliometric analysis

Cai,

Chen,

Wang

et al. 2024

World J Clin Cases

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BACKGROUND Malignant tumors are one of the leading causes of death worldwide, imposing a substantial economic and social burden. Early detection is the key to improving cure rates and reducing mortality rates, which requires the development of sensitive early detection technologies. Signal amplification techniques play a crucial role in aptamer-based early detection of tumors and are increasingly garnering attention from researchers. AIM To investigate the current research status, developmental trajectories, and hotspots in signal amplification for aptamer-based tumor detection through bibliometric analysis. METHODS English publications pertaining to signal amplification in aptamer-based tumor detection were retrieved from the Web of Science Core Collection database. VOSviewer and CiteSpace software were employed to analyze various information within this field, including countries, institutions, authors, co-cited authors, journals, co-cited journals, cited references, and keywords. RESULTS A total of 757 publications were included in this study. China accounted for 85.47% of all publications, with Nanjing University (China) emerging as the institution with the highest publication output. The most influential authors and journals were Hasanzadeh M. from Iran and "Biosensors and Bioelectronics ", respectively. Exosomes and carcinoembryonic antigen (CEA) stood out as the most researched tumor-related molecules. Currently, the predominant signal amplification technique, nanomaterial, and signal transduction method were identified as hybridization chain reactions, gold nanoparticles, and electrochemical methods, respectively. Over the past 3 years, exosomes, CEA, electrochemical biosensors, and nanosheets have emerged as research hotspots, exhibiting a robust burst of intensity. CONCLUSION This study is the first bibliometric analysis of literature on signal amplification in aptamer-based tumor detection and elucidates the current status, hotspots, and prospective research directions within this realm. Additionally, it provides an important reference for researchers.

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Section: Discussionmentioning

confidence: 99%

Trends and frontiers in signal amplification for aptamer-based tumor detection: A bibliometric analysis

Cai,

Chen,

Wang

et al. 2024

World J Clin Cases

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“…Consequently, R1 and R2 hybridized with each other to form a stable DNA duplex (R1–R2) and a complete trigger chain (T) was built, subsequently initiating signal conversion and amplification. Many cascaded amplification assays for EVs have been proposed to achieve higher sensitive detection. ,, However, amplification reactions usually occurred on the surface of EVs, and the amplification efficiency was limited due to steric hindrance. Inspired by Li et al, we moved the amplification reaction from EVs to the solution.…”

mentioning

confidence: 99%

Dual-Recognition-Mediated Autocatalytic Amplification Assay for the Subpopulations of PD-L1 Positive Extracellular Vesicle

Ren,

Ge,

Tang

et al. 2024

Anal. Chem.

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The PD-L1 protein on extracellular vesicles (EVs) is a promising biomarker for tumor immunotherapy. However, PD-L1+ EVs have various cell origins, so further analysis of the subpopulations is essential to help understand better their relationship with tumor immunotherapy. Different from the previous work which focus on the level of total PD-L1+ EVs expression, we, herein, report a dual-recognition mediated autocatalytic amplification (DRMAA) assay to detect the PD-L1 derived from tumors (EpCAM+), immune T cells (CD3+), and total (Lipids) EVs, respectively. The DRMAA assay employed proximity hybridization to construct a complete trigger sequence and then catalyzed the cross-hybridization of three hairpin probes, producing a three-way DNA junction (3-WJ) structure carrying the newly exposed trigger sequence. The 3-WJ complex subsequently initiated an autocatalytic amplification reaction and higher sensitivity than the traditional catalytic hairpin assembly assay was obtained. It was found that the EpCAM+ and PD-L1+ EVs were more effective than others in distinguishing lung cancer patients from healthy people. Surprisingly, the CD3+ and PD-L1+ EVs in lung cancer patients were also upregulated, indicating that immune cell-derived PD-L1+ EVs are also non-negligible marker in a tumor microenvironment. Our results suggested that the DRMAA assay would improve the study of subpopulations of PD-L1+ EVs to provide new insights for cancer immunotherapies.

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Immuno‐Rolling Circle Amplification (Immuno‐RCA): Biosensing Strategies, Practical Applications, and Future Perspectives

Zhang,

Bai,

Zou

et al. 2024

Adv Healthcare Materials

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In the rapidly evolving field of life sciences and biomedicine, detecting low‐abundance biomolecules, and ultraweak biosignals presents significant challenges. This has spurred a rapid development of analytical techniques aiming for increased sensitivity and specificity. These advancements, including signal amplification strategies and the integration of biorecognition events, mark a transformative era in bioanalytical precision and accuracy. A prominent method among these innovations is immuno‐rolling circle amplification (immuno‐RCA) technology, which effectively combines immunoassays with signal amplification via RCA. This process starts when a targeted biomolecule, such as a protein or cell, binds to an immobilized antibody or probe on a substrate. The introduction of a circular DNA template triggers RCA, leading to exponential amplification and significantly enhanced signal intensity, thus the target molecule is detectable and quantifiable even at the single‐molecule level. This review provides an overview of the biosensing strategy and extensive practical applications of immuno‐RCA in detecting biomarkers. Furthermore, it scrutinizes the limitations inherent to these sensors and sets forth expectations for their future trajectory. This review serves as a valuable reference for advancing immuno‐RCA in various domains, such as diagnostics, biomarker discovery, and molecular imaging.

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DNA four-way junction-driven dual-rolling circle amplification sandwich-type aptasensor for ultra-sensitive and specific detection of tumor-derived exosomes

Cited by 12 publications

References 70 publications

Trends and frontiers in signal amplification for aptamer-based tumor detection: A bibliometric analysis

Trends and frontiers in signal amplification for aptamer-based tumor detection: A bibliometric analysis

Dual-Recognition-Mediated Autocatalytic Amplification Assay for the Subpopulations of PD-L1 Positive Extracellular Vesicle

Immuno‐Rolling Circle Amplification (Immuno‐RCA): Biosensing Strategies, Practical Applications, and Future Perspectives

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