DNA fragmentation status in patients with necrozoospermia

Brahem, Sonia; Jellad, Sonia; Ibala, Samira; Saâd, Ali; Mehdi, Meriem

doi:10.3109/19396368.2012.710869

Cited by 16 publications

(23 citation statements)

References 32 publications

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“…In agreement with this finding, Samplaski et al [27] concluded that DFI testing may not be necessary in men with high (≥75%) and low (≤30%) levels of sperm vitality. In addition, our results support the results of Brahem et al [24], who found high levels of sperm DNA fragmentation in patients with necrozoospermia, a condition in which the proportion of non-viable spermatozoa in the ejaculate is increased significantly to more than 42%.…”

Section: Discussionsupporting

confidence: 92%

“…Sperm vitality and progressive motility exhibited the most significant associations, which could be explained by the significant role of epididymal function in sperm pathology. Due to long exposure to overproduced reactive oxygen species (ROS) at epididymal level by both leukocytic and nonleukocytic cells, such as immature spermatozoa [20], epididymal epithelial cells [21] and spermiophages [22], ROS-induced sperm membrane peroxidation results in loss of sperm motility, membrane integrity, and consequently, loss of sperm vitality [23][24][25]. Moreover, ROS capable of penetrating sperm membrane can impair sperm DNA integrity either directly by attacking DNA backbone and by forming abasic sites and DNA adducts or indirectly by triggering the apoptotic cascade through the activation of caspases and endonucleases [26].…”

Section: Discussionmentioning

confidence: 99%

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Standard Semen Parametersvs.Sperm Kinematics to Predict Sperm DNA Damage

Aghazarian

Huf

Pflüger

et al. 2021

World J Mens Health

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Purpose: The aims of this study were to associate sperm kinematics and standard semen parameters with sperm DNA damage and to evaluate whether the addition of sperm kinematics improve the multivariable prediction of sperm DNA fragmentation compared to standard semen parameters alone. Materials and Methods: We evaluated sperm kinematics, standard semen parameters, and DNA fragmentation index (DFI) in 122 men. Univariate and multivariate logistic regression models were fitted to evaluate the association of sperm kinematics and standard semen parameters with pathologically damaged sperm DNA (DFI≥26%), and receiver operating characteristics (ROC) curves were calculated for these models. Results: On univariate analyses, average velocity, curvilinear velocity, straight-line velocity, straightness (STR), beat-cross frequency (BCF), and the percentage of progressive motile sperm cells (PPMS) were significantly associated with pathologically damaged sperm DNA. Likewise, among standard semen parameters, sperm concentration, progressive motility, normal morphology, and vitality were found to be linked with sperm DNA damage. On the multivariate analysis, vitality was the strongest predictor of pathologically damaged sperm DNA with an area under the ROC curve (AUROC) of 88.3%. Adding STR, BCF, and PPMS to vitality increased the AUROC to the significant extent of 91.5%. Conclusions: Sperm vitality is the most accurate routine-based laboratory test for the prediction of pathologically damaged sperm DNA, but the addition of sperm kinematics increases its accuracy. Both standard semen parameters and sperm kinematics are complementary in predicting pathologically damaged sperm DNA, and might serve as a new tool to screen for fertile men.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Standard Semen Parametersvs.Sperm Kinematics to Predict Sperm DNA Damage

Aghazarian

Huf

Pflüger

et al. 2021

World J Mens Health

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“…While the relationship between sperm concentration and SDF in subfertile men appears to vary, a correlation between SDF and sperm viability has been demonstrated [15]. Although the mechanisms have not been fully recognized, SDF is seen in mature, viable sperm.…”

Section: Introductionmentioning

confidence: 99%

Abnormal Human Sperm Parameters Contribute to Sperm DNA Fragmentation in Men with Varicocele

Park

Lee

Choi

et al. 2018

World J Mens Health

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PurposeThis study was performed to evaluate and compare threshold sperm parameters and sperm DNA fragmentation index (DFI), and further analyzed whether sperm DFI could be predicted from sperm parameters in men with varicocele.Materials and MethodsA total of 157 semen samples underwent both semen analysis and sperm DNA fragmentation (SDF) testing in men with varicocele. Sperm parameters were assessed using the World Health Organization guidelines. SDF testing was performed using the Halosperm kit. Sperm parameters and sperm DFI results were compared.ResultsThe overall sperm parameter results and sperm DFI showed normal values; however, the morphology value was at the lower limit of normal. High sperm DFI was associated with significantly lower motility and viability (p<0.001, respectively). Sperm motility and morphology were significantly higher in the higher sperm count group compared to the lower sperm count group (p<0.05), while sperm DFI was higher in the lower sperm count group (p<0.05). Sperm count and viability and sperm DFI were significantly associated with the quality of sperm motility (p<0.001). Sperm motility and sperm DFI were significantly different (p<0.001) between normal and abnormal sperm viability groups. Between normal and abnormal sperm morphology groups, sperm count, motility, and sperm DFI showed significant differences (p<0.001).ConclusionsIn this study, a correlation between SDF and sperm parameters was confirmed in men with varicocele. SDF may be contributing factors to sperm motility, viability, and morphology. Abnormal sperm count, motility, and viability showed high sperm DFI. Therefore, lower sperm parameters were indicative of increasing SDF in men with varicocele.

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“…DNA breaks are among primary factors inducing spermatozoa apoptosis (12,31). The literature has demonstrated a clear link between DNA fragmentation and sperm viability (6).…”

Section: Resultsmentioning

confidence: 99%

Influence of addition of cobalamin to the extender on the post-thaw motility, viability, and DNA integrity of bovine ejaculate

Mizera¹,

Kuczaj²,

Szul³

et al. 2019

Medycyna Weterynaryjna

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The present study was undertaken to investigate the effect of various concentrations of cobalamin (vitamin B12) in Bioexcell® extender on the spermatozoa quality of Simmental bulls. The semen was collected from 12 bulls by means of an artificial vagina. Vitamin B12 at concentrations of 1.50, 2.50, 3.50, and 5.00 mg/mL was added to bovine Bioexcell® extender. The semen aliquots were cooled and preserved at 4°C. Their quality was evaluated during pre-freezing, and then the cooled semen samples were packaged into 0.25 ml straws. The straws were frozen in the vapor of liquid nitrogen, and stored at -196ºC in a container. The straws were thawed one day later, and the characteristics of spermatozoa were examined. The results showed that the effect of vitamin B12 on the viability, DNA fragmentation and motility of spermatozoa was significant under both pre- and post-freezing conditions (p <0.05), and revealed that supplementation of the extender with B12 improved the post-thaw spermatozoa quality in bulls.

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DNA fragmentation status in patients with necrozoospermia

Cited by 16 publications

References 32 publications

Standard Semen Parametersvs.Sperm Kinematics to Predict Sperm DNA Damage

Standard Semen Parametersvs.Sperm Kinematics to Predict Sperm DNA Damage

Abnormal Human Sperm Parameters Contribute to Sperm DNA Fragmentation in Men with Varicocele

Influence of addition of cobalamin to the extender on the post-thaw motility, viability, and DNA integrity of bovine ejaculate

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