DNA: Gold nanoparticles designed for mRNA sensing in cells: imaging of the gold nanoparticles using two photon photoluminescence spectroscopy.

Kyriazi, Maria Eleni; Muskens, Otto L.; Kanaras, Antonios G.

doi:10.1117/12.2502724

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…Our group have developed for the first time an IOC device coupled with a localized surface plasmon resonance (LSPR) sensing module to monitor on-line and label-free the insulin secretion in pancreatic islets [102]. These optical biosensors present the advantage of being highly sensitive, enabling online, label-free, and cost-effective sensing and have shown great capability to detect all kinds of molecular biomarkers [103][104][105]. Unlike other IOC devices, based on multiple tiny wells to trap the islets [65,68,70,106], we have developed a 3D heterogeneous porous polymeric cryogel scaffold to spatially organize the islets.…”

Section: Label-free Detection Of Insulin Secretionmentioning

confidence: 99%

Islet-on-a-chip for the study of pancreatic β-cell function

Rodríguez‐Comas

Ramón‐Azcón

2021

In vitro models

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Diabetes mellitus is a significant public health problem worldwide. It encompasses a group of chronic disorders characterized by hyperglycemia, resulting from pancreatic islet dysfunction or as a consequence of insulin-producing β-cell death. Organ-on-a-chip platforms have emerged as technological systems combining cell biology, engineering, and biomaterial technological advances with microfluidics to recapitulate a specific organ’s physiological or pathophysiological environment. These devices offer a novel model for the screening of pharmaceutical agents and to study a particular disease. In the field of diabetes, a variety of microfluidic devices have been introduced to recreate native islet microenvironments and to understand pancreatic β-cell kinetics in vitro. This kind of platforms has been shown fundamental for the study of the islet function and to assess the quality of these islets for subsequent in vivo transplantation. However, islet physiological systems are still limited compared to other organs and tissues, evidencing the difficulty to study this “organ” and the need for further technological advances. In this review, we summarize the current state of islet-on-a-chip platforms that have been developed so far. We recapitulate the most relevant studies involving pancreatic islets and microfluidics, focusing on the molecular and cellular-scale activities that underlie pancreatic β-cell function.

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Section: Label-free Detection Of Insulin Secretionmentioning

confidence: 99%

Islet-on-a-chip for the study of pancreatic β-cell function

Rodríguez‐Comas

Ramón‐Azcón

2021

In vitro models

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“…Among the different existing transduction methods, optical biosensors have the advantage of being highly sensitive, enabling label-free, cost-effective, and real-time sensing. As a well-studied optical sensing scheme, localized surface plasmon resonance (LSPR)-based sensors, which exploit the unique properties of noble metal nanostructures, have shown a great ability to detect all kinds of molecular biomarkers (proteins [ 17 ], peptides [ 18 ], mRNA [ 19 ], DNA [ 20 , 21 ], and miRNA [ 22 ]) in biological samples. The ease of optical transduction and the compact nature of LSPR sensors means their integration into fully automated microfluidic devices to perform multiplexed quantitative detection can be achieved [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

In Situ LSPR Sensing of Secreted Insulin in Organ-on-Chip

et al. 2021

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Organ-on-a-chip (OOC) devices offer new approaches for metabolic disease modeling and drug discovery by providing biologically relevant models of tissues and organs in vitro with a high degree of control over experimental variables for high-content screening applications. Yet, to fully exploit the potential of these platforms, there is a need to interface them with integrated non-labeled sensing modules, capable of monitoring, in situ, their biochemical response to external stimuli, such as stress or drugs. In order to meet this need, we aim here to develop an integrated technology based on coupling a localized surface plasmon resonance (LSPR) sensing module to an OOC device to monitor the insulin in situ secretion in pancreatic islets, a key physiological event that is usually perturbed in metabolic diseases such as type 2 diabetes (T2D). As a proof of concept, we developed a biomimetic islet-on-a-chip (IOC) device composed of mouse pancreatic islets hosted in a cellulose-based scaffold as a novel approach. The IOC was interfaced with a state-of-the-art on-chip LSPR sensing platform to monitor the in situ insulin secretion. The developed platform offers a powerful tool to enable the in situ response study of microtissues to external stimuli for applications such as a drug-screening platform for human models, bypassing animal testing.

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