DNA homology between Saccharopolyspora strains and other erythromycin-producing actinomycetes

Stanzaic, Richard; Matsushima, Patti; Baltz, Richard H.; Schoner, Brigitte E.

doi:10.1099/00221287-136-9-1899

Cited by 6 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another example is erythromycin production in Saccharopolyspora erythraea. There are several independent isolates of S. erythraea that produce erythromycin, 36 and analysis of 16S rRNA sequences from four strains showed pairwise divergence times ranging from 7 to 73 Myr ago (V Miao and RH Baltz, unpublished). This range of divergence within a single species provides a good system to compare the divergence of erythromycin biosynthetic genes and enzymes relative to primary metabolic functions.…”

Section: Calculation Of Time To Last Common Ancestormentioning

confidence: 99%

Genomics and the ancient origins of the daptomycin biosynthetic gene cluster

Baltz¹

2010

J Antibiot

View full text Add to dashboard Cite

Daptomycin is a clinically useful lipopeptide antibiotic produced by Streptomyces roseosporus. The antibiotic is assembled by a nonribosomal peptide synthetase (NRPS) mechanism, and the cyclized tridecapeptide contains three non-proteinogenic L-amino acids: ornithine (Orn), 3-methyl-glutamic acid (3mGlu) and kynurinine (Kyn). Daptomycin also has three D-amino acids. The two genes that encode proteins involved in the formation of 3mGlu and Kyn have no known orthologs, and hence they are good probes to search for daptomycin-like gene clusters among newly sequenced actinomycete genomes. A recent search with these genes revealed a daptomycin-like gene cluster in Saccharomonospora viridis, a causative agent for farmer's lung disease. S. viridis has a genome of 4.3 Mb, which is about one half the size of Streptomyces genomes. Searches for other NRPS and polyketide synthase (PKS) genes revealed only one other NRPS gene and no PKS genes in S. viridis. The orthologous lipopeptide biosynthetic genes and gene products in S. viridis and S. roseosporus have diverged extensively from a last common ancestor dating back to a pathway that had evolved over 1 billion years ago.

show abstract

Section: Calculation Of Time To Last Common Ancestormentioning

confidence: 99%

Genomics and the ancient origins of the daptomycin biosynthetic gene cluster

Baltz¹

2010

J Antibiot

View full text Add to dashboard Cite

show abstract

“…Others antibiotics produced by other genera were also reported by genus Micromonospora. These include Primycin [41,236], Rifamycins [41,237,238], Erythromycin B [41,239].…”

Section: (C) Rosamicin Have Anmentioning

confidence: 99%

Recent progress on the development of antibiotics from the genus Micromonospora

Boumehira

El‐Enshasy

Hacène

et al. 2016

Biotechnol Bioproc E

View full text Add to dashboard Cite

The emergence of large number of antimicrobial-resistant organisms has given alarming magnitude. Nature is historically the source of drugs, and microorganisms have provided a significant number of antibiotic compounds, which are used every day in the treatment of many infectious diseases. However, the introduction to the pharmaceutical market of new therapeutic molecules has largely decreased during the last two decades. In this review, antibiotics from the genus Micromonospora are recognized as potential biofactory for new antibiotic production. The Micromonospora has been deeply studied and more than 100 antibiotics isolated from different Micromonospora strains. In addition, comprehensive information about the recent development in the field of analytical, biological and bioinformatics screening tools, which recently used in the discovery of new therapeutic compounds, are provided. It is widely believed that reviving old antibiotics produced by Micromonospora is possible and the study of this genus is still interesting for novel bioactive molecules discovery.

show abstract

Transposition and transduction of plasmid DNA inStreptomyces spp.

Hahn¹,

Solenberg²,

McHenney³

et al. 1991

Journal of Industrial Microbiology

View full text Add to dashboard Cite

To expand the application of molecular genetics to many different streptomycete species, we have been developing two potentially widely applicable methodologies: transposon mutagenesis and plasmid transduction. We constructed three transposons from the Streptomyces lividans insertion sequence IS493. Tn5096 and Tn5097 contain an apramycin resistance gene inserted in different orientations between the two open reading frames of IS493. These transposons transpose from different plasmids into many different sites in the Streptomyces griseofuscus chromosome and into its resident linear plasmids. Tn5099 contains a promoterless xylE gene and a hygromycin-resistance gene inserted in IS493 close to one end. Tn5099 transposes in S. griseofuscus giving operon fusions in some cases that drive expression of the xylE gene product, catechol deoxygenase, giving yellow colonies in the presence of catechol. We have also developed plasmid vectors that can be transduced into many streptomycete species by bacteriophage FP43. We describe the characterization of FP43 and mapping of several bacteriophage functions. The region of cloned FP43 DNA essential for plasmid transduction includes the origin for headful packaging.

show abstract

DNA homology between Saccharopolyspora strains and other erythromycin-producing actinomycetes

Cited by 6 publications

References 23 publications

Genomics and the ancient origins of the daptomycin biosynthetic gene cluster

Genomics and the ancient origins of the daptomycin biosynthetic gene cluster

Recent progress on the development of antibiotics from the genus Micromonospora

Transposition and transduction of plasmid DNA inStreptomyces spp.

Contact Info

Product

Resources

About