DNA hydrolysis by stable metal complexes

Abstract

Stable cobalt(rI1) complexes promote hydrolysis of the DNA phosphodiester backbone.

Hydrolysis of plasmid DNA catalyzed by Co(III) complex of cyclen attached to polystyrene

“…À6 s À1 for 1 mM C o at 37 C and pH 7.5] of CoCyc toward the supercoiled pUC18 measured in this study is comparable to the reported value (k o =1Â10 À5 s À1 for 1 mM C o at 37 C and pH 7.6) 6 for that toward supercoiled pUC9. When the supercoiled DNA was incubated with the Cyc-containing resins without Co(III) ion, cleavage of the DNA was negligible under the conditions of the kinetic measurements.…”

“…A mechanism (2) has been proposed 6 for the catalytic action of the Co(III) complexes: the Co(III) center binds the phosphate anion of the phosphodiester linkage and the adjacent Co(III)-bound hydroxide ion makes intramolecular attack at the phosphorus atom of the bound substrate.…”

DNA Binding and Cleavage Studies of Dichlorido[Bis(2-Ethyl-5-Methyl-1H-Imidazol-4-yl-κN3)Methane]Cobalt(II) Monohydrate

“…These studies are also important to understand the toxicity of metal based drugs [7]. Cobalt complexes have gained importance for their applicability in the biological field [8][9][10]. Some of the previous reports show that hexamine cobalt complexes induce DNA condensation [11,12] and can be used as RNA hairpins probe [13,14].…”

Ternary Zinc(II)-Dipeptide Complexes for the Hydrolytic Cleavage of DNA at Physiological pH

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The literature suggests that only stable, tetra-coordinated metal complexes are capable of cleaving phosphodiester bonds [12]. We, therefore, decided to synthesize various stable Zn II complexes, as they are known to play an important role in phosphodiester cleavage [13], and to study their DNA-cleavage abilities.

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