DNA in Heterochromatin Cytophotometric Pattern Recognition Image Analysis among Cell Nuclei in Duct Epithelium and in Carcinoma of the Human Breast

Sandritter, W.; Kiefer, G.; Kiefer, R.; Salm, R. van der; Moore, Gary; Grimm, H

doi:10.1016/s0005-8165(74)80031-4

Cited by 32 publications

(5 citation statements)

References 8 publications

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“…(1983), McDivitt et al (1986) and Thorud et al (1986) as well as the cytophotometric studies of Emson & Kirk (1966), Tzuo er al. (1971( ), Sandritter et al (1974 and Fossa et at. (1983).…”

Section: Discussionmentioning

confidence: 97%

Feulgen DNA content and mitotic activity in proliferative breast disease. A comparison with ductal carcinoma in situ

et al. 1987

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Nuclear Feulgen DNA content was measured by cytophotometry and the number of mitoses per 40 high power fields was determined in hyperplastic and atypical hyperplastic lesions of fibrocystic disease in 18 patients, in ductal carcinoma in situ in 14 patients and in ductal carcinoma in situ associated with infiltrating carcinoma in 11 patients. These parameters were also investigated in the hyperplastic lesions accompanying ductal carcinoma in situ and ductal carcinoma in situ associated with infiltrating carcinoma. The nuclear Feulgen DNA content could not discriminate between atypical hyperplasia and ductal carcinoma in situ. Although differences in the mitotic count between hyperplastic and atypical breast lesions were not statistically significant, there was a statistically significant greater mitotic count in ductal carcinoma in situ alone or associated with infiltrating carcinoma. These findings suggest that the mitotic count is useful for the differential diagnosis between atypical hyperplasia and ductal carcinoma in situ. In addition, hyperplastic lesions associated with ductal carcinoma in situ, with or without infiltrating carcinoma, exhibited a statistically significant higher mitotic count than those in benign fibrocystic disease. Hyperplastic breast lesions exhibiting high mitotic counts may indicate the presence of a neighbouring ductal malignancy and suggest an increased proliferative activity in breast tissue in the neighbourhood of malignancy.

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“…(1983), McDivitt et al (1986) and Thorud et al (1986) as well as the cytophotometric studies of Emson & Kirk (1966), Tzuo er al. (1971( ), Sandritter et al (1974 and Fossa et at. (1983).…”

Section: Discussionmentioning

confidence: 97%

Feulgen DNA content and mitotic activity in proliferative breast disease. A comparison with ductal carcinoma in situ

et al. 1987

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“…As expected, the HP1 signal was unevenly distributed in nuclei and was concentrated in discrete foci 51, 52 ( Figure 2A) . It has been reported that for many eukaryotes, heterochromatin constitutes 10–20% of the genome 53, 54 . These reports prompted us to set an initial threshold for identifying heterochromatin locations as the 20% brightest HP1 signals and euchromatin as the other 80% of each nucleus.…”

Section: Resultsmentioning

confidence: 99%

The consequences of differential origin licensing dynamics in distinct chromatin environments

Liu

Kedziora

Song

et al. 2021

Preprint

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MCM complexes are loaded onto chromosomes to license DNA replication origins in G1 phase of the cell cycle, but it is not yet known how mammalian MCM complexes are adequately distributed to both euchromatin and heterochromatin. To address this question, we combined time-lapse live-cell imaging with fixed cell immunofluorescence imaging of single human cells to quantify the relative rates of MCM loading in heterochromatin and euchromatin at different times within G1. We report here that MCM loading in euchromatin is faster than in heterochromatin in very early G1, but surprisingly, heterochromatin loading accelerates faster than euchromatin in middle and late G1. These different loading dynamics require ORCA-dependent differences in ORC distribution during G1. A consequence of heterochromatin origin licensing dynamics is that cells experiencing a truncated G1 phase from premature Cyclin E expression enter S phase with underlicensed heterochromatin, and DNA damage accumulates preferentially in heterochromatin in the subsequent S/G2 phase. Thus G1 length is critical for sufficient MCM loading, particularly in heterochromatin, to ensure complete genome duplication and to maintain genome stability.

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“…However, the frequency of this phenotype was observed to be significantly increased in fasted nymphs and in specimens subjected to heat shock [ 10 , 11 ]. This phenomenon, which affects the heterochromatin in other cell systems and under different physiological conditions has been hypothesized to represent an attempt to activate silent genes possibly present in this chromatin compartment and achieve cell survival [ 38 , 39 ].…”

Section: Heterochromatin and Nuclear Phenotypesmentioning

confidence: 99%

Nuclear Morphofunctional Organization and Epigenetic Characteristics in Somatic Cells of T. infestans (Klug, 1834)

Mello

2023

Pathogens

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Triatoma infestans (Klug) is an insect recognized as not only an important vector of South American trypanosomiasis (Chagas disease) but also a model of specific cellular morphofunctional organization and epigenetic characteristics. The purpose of the present review is to highlight certain cellular processes that are particularly unveiled in T. infestans, such as the following: (1) somatic polyploidy involving nuclear and cell fusions that generate giant nuclei; (2) diversification of nuclear phenotypes in the Malpighian tubules during insect development; (3) heterochromatin compartmentalization into large bodies with specific spatial distribution and presumed mobility in the cell nuclei; (4) chromatin remodeling and co-occurrence of necrosis and apoptosis in the Malpighian tubules under stress conditions; (5) epigenetic markers; and (6) response of heterochromatin to valproic acid, an epidrug that inhibits histone deacetylases and induces DNA demethylation in other cell systems. These cellular processes and epigenetic characteristics emphasize the role of T. infestans as an attractive model for cellular research. A limitation of these studies is the availability of insect supply by accredited insectaries. For studies that require the injection of drugs, the operator’s dexterity to perform insect manipulation is necessary, especially if young nymphs are used. For studies involving in vitro cultivation of insect organs, the culture medium should be carefully selected to avoid inconsistent results.

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DNA in Heterochromatin Cytophotometric Pattern Recognition Image Analysis among Cell Nuclei in Duct Epithelium and in Carcinoma of the Human Breast

Cited by 32 publications

References 8 publications

Feulgen DNA content and mitotic activity in proliferative breast disease. A comparison with ductal carcinoma in situ

Feulgen DNA content and mitotic activity in proliferative breast disease. A comparison with ductal carcinoma in situ

The consequences of differential origin licensing dynamics in distinct chromatin environments

Nuclear Morphofunctional Organization and Epigenetic Characteristics in Somatic Cells of T. infestans (Klug, 1834)

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