1985
DOI: 10.1038/bjc.1985.228
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DNA index of ovarian carcinomas from 56 patients: in vivo in vitro studies

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Cited by 27 publications
(2 citation statements)
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“…The cell suspension was obtained by mechanical disaggregation of the tumor tissue through an 18 x ?h needle and resuspended in HBSS; 200-400 p1 cell suspensions (1-2 x lo6 cells/ml) were stained by adding 2 ml of propidium iodide solution (50 pg/ml in 0.1% sodium citrate) plus 25 pl RNase (0.5 mg/ml) (Calbiochem Behring, La Jolla, CA) plus 25 p1 Nonidet P.40 (Sigma) for at least 60 min at room temperature. Human and mouse leukocytes from freshly collected blood were used as standards to determine the DNA content of human and xenograft tumors respectively (Erba et al, 1985). Ploidy was expressed as DNA index, representing the ratio between the G1 peak of tumor cells and the GdGl peak of leukocytes (Barlogie et al, 1983).…”
Section: Flow Cytornetrymentioning
confidence: 99%
“…The cell suspension was obtained by mechanical disaggregation of the tumor tissue through an 18 x ?h needle and resuspended in HBSS; 200-400 p1 cell suspensions (1-2 x lo6 cells/ml) were stained by adding 2 ml of propidium iodide solution (50 pg/ml in 0.1% sodium citrate) plus 25 pl RNase (0.5 mg/ml) (Calbiochem Behring, La Jolla, CA) plus 25 p1 Nonidet P.40 (Sigma) for at least 60 min at room temperature. Human and mouse leukocytes from freshly collected blood were used as standards to determine the DNA content of human and xenograft tumors respectively (Erba et al, 1985). Ploidy was expressed as DNA index, representing the ratio between the G1 peak of tumor cells and the GdGl peak of leukocytes (Barlogie et al, 1983).…”
Section: Flow Cytornetrymentioning
confidence: 99%
“…After 48 h, cells were harvested with trypsin-EDTA. Cellular DNA was stained with propidium iodide after RNAse digestion on the fixed cells (Erba, Vaghi, Pepe et al 1985). Flow cytometry analysis was performed using an EPICS-C flowcytometer (Coulter Electronics Ltd, Luton, Beds, U.K.).…”
Section: Effects Of Ifn On Dna Synthesismentioning
confidence: 99%