DNA interactions and anticancer screening of copper(II) complexes of N-(methylpyridin-2-yl)-amidino-O-methylurea

The effect of gallic acid on the copper(ii) complex ofN-(methylpyridin-2-yl)-amidino-O-methylurea: crystal structure, DNA interactions,in vitrocytotoxicity and antibacterial activity

“…According to the K b values (Table 3), the binding potential of 1-HGA is slightly better than that of 1 . 13 However, their K b values are lower than those of the classical intercalator ethidium bromide (EB) because EB has a lot of aromatic rings, which allows it to be easily inserted between DNA base pairs via intercalation mode, as opposed to 1-HGA , which interacts in a non-intercalation mode, making binding with DNA difficult. 69 In comparison with the relevant complexes, [Cr(III)GA 2 ·2H 2 O], 70 both 1 and 1-HGA show significantly greater binding abilities than chromium complexes.…”

“…12 In 2017, we reported that the DNA binding abilities of 1 occur via non-intercalation modes with K b = 2.81 × 10 5 M −1 and that it causes DNA cleavage through the oxidative mechanism. 13 In addition, the anticancer activities of 1 show cytotoxicity toward lung (NCI-H187) and oral (KB) cancer cell lines. These results suggest that the complex, 1 , has the potential to be developed as a DNA-interacting agent.…”

Copper(II) Compounds of 4‐Nitrobenzohydrazide with Different Anions (ClO4−, NO3− and Br−): Synthesis, Characterization, DFT Calculations, DNA Interactions and Cytotoxic Properties

“…The stock solutions of the complexes were prepared in HEPES buffer (40 10). [51] % DNA Form I ¼ ½Volume of DNA Form I= Total volume of all DNA forms� � 100 (10) Where volume is intensity × area of the visualized DNA bands in agarose gel. Additionally, the DNA cleavage process in the presence of ascorbic acid (30 μM) was carried out in the complex concentration of 1, 10, 50 and 100 μM for 1 and 1, 10 and 50 for 2 and 3.…”