DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation

Imaeda, T; Kirchheimer, W. F.; Barksdale, Lane

doi:10.1128/jb.150.1.414-417.1982

Cited by 53 publications

(17 citation statements)

References 24 publications

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“…M. vaccae DNA has a G+C content similar to the reported values for other mycobacterial DNAs, which fall into two groups: one with G+C contents of 64 to 66.4% and the other with G+C contents of 67 to 70% (39). In contrast, M. leprae DNA has a G+C content significantly different from those of other mycobacteria, as has been reported by Imaeda et al (19). "M. lufu" DNA also has a G+C content that is lower than those of other mycobacterial DNAs.…”

Section: Resultssupporting

confidence: 53%

Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae

Clark‐Curtiss¹,

Jacobs²,

Docherty³

et al. 1985

J Bacteriol

170

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Molecular analysis of DNA from Mycobacterium leprae, "Mycobacterium lufu," and Mycobacterium vaccae has demonstrated that the G+C (guanine plus cytosine) contents of the DNAs are 56, 61, and 65%, respectively, and that the genome sizes are 2.2 x 109, 3.1 x 109, and 3.1 x 109 daltons, respectively. Because CLARK-CURTISS ET AL. J. BACTERIOL. TABLE 1. Bacterial strains Strain Genotype Source or reference E. coli K-12 X289 F-tte-J prototroph 11 E. coli K-12 x925 F-thr-1 ara-13 leu-6 azi-8 tonA2 lacYl minAl Single colony isolate of P678-54; 1 ginV44 gal-6 A-minB2 rpsL35 malAl xyl-7 mtl-2 thi-i E. coli K-12 X1849 F-tonA53 dapD8 minAl purE41 ginV42 A(gal-17 uvrB)40 A-minB2 his-53 gyrA25 metC65 oms-i tte-J A(bioH-asd)29 ilv-277 cycB2 cycAl hsdR2 E. coli K-12 X2001 F-AaraC766 tonA53 dapD8 proA370 AlacZ39 minAl 7 A(gal-chiD)69 X-tyrT58 AgalUi83 AtrpE5 minB2 rfb-2 recA56 relAl AthyA57 endAI oms-i Aasd4 rpoB402 cycB2 cycAl hsdR2 E. coli K-12 X2819 F-lacYl ginV44 galK2 gaIT22 (A cI857 b2 red3 S7) This paper recA56 AthyA57 metBI hsdR2 M. leprae Wild type Armadillo liver #29 infected with M. leprae pooled from seven patients; received from C.

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Section: Resultssupporting

confidence: 53%

Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae

Clark‐Curtiss¹,

Jacobs²,

Docherty³

et al. 1985

J Bacteriol

170

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“…Current genetic information suggests that M. leprae is only minimally related to other mycobacteria according to guanine plus cytosine content (Clark-Curtiss etal.. 1985), genome size Imaeda et al, 1982), and DNA homoiogy as judged by fluid-phase reassociation kinetics (Imaeda etal., 1982) or solid-phase hybridization kinetics (Grosskinsky ef al., 1989). A comparison between M. leprae isolates by total genomic hybridization studies showed high levels of homoiogy, suggesting a high degree of relatedness between isolates (Imaeda etal.. 1982).…”

Section: Introductionmentioning

confidence: 99%

Geographically distinct isolates of Mycobacterium leprae exhibit no genotypic diversity by restriction fragment‐length polymorphism analysis

Williams

Gillis

Portaels

1990

Molecular Microbiology

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Differentiation of microorganisms for taxonomic purposes is based primarily on phenotypic characteristics, which are the direct or cumulative result of gene expression. Since expression of phenotypic characteristics usually relies on in vitro growth of a microorganism, non-cultivable organisms, such as Mycobacterium leprae, present major problems for the identification of potential variants based on phenotypic similarities or differences between individual isolates. We have employed the use of restriction fragment-length polymorphism (RFLP) analysis of chromosomal DNA of M. leprae isolates, including human isolates from geographically distinct regions of the world and isolates from a Sooty Mangabey monkey and an armadillo, to assess the relatedness among these isolates. Restriction endonuclease (EcoRI, BstEII, PstI, and PvuI) digests of chromosomal DNA were analysed using DNA probes encoding all or part of the 12 kD, 18 kD, 28 kD, 65 kD and 70 kD proteins of M. leprae as well as a probe containing an M. leprae-specific sequence repeated up to 20 times in the M. leprae chromosome. Comparison of the resulting autoradiographs showed that the RFLP patterns were all identical, indicating that these isolates contained no polymorphism with respect to the restriction endonuclease sites analysed. In addition, RFLP patterns of two separate human M. leprae isolates remained unchanged after three cycles of experimental infection in the armadillo model. These results indicated that the M. leprae isolates tested in this study were indistinguishable at the genotypic level, strongly suggesting homogeneity among members of this species.

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“…However, the possibility cannot be excluded that a portion of the antibodies is directed to cross-reactive determinants and results from a response to other mycobacteria. The established DNA sequences had a G+C content that was much higher than that reported for the M. leprae genome and more comparable to those of a number of other mycobacteria (10,17). Although the sequenced parts of the genes may not be representative for the complete genes, this might indicate that the expressed M. leprae determinants are not specific for this bacillus but could be common in a number of other pathogenic and nonpathogenic mycobacteria.…”

Section: Vol 58 1990mentioning

confidence: 76%

Selection and characterization of recombinant clones that produce Mycobacterium leprae antigens recognized by antibodies in sera from household contacts of leprosy patients

et al. 1990

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A Mycobacterium leprae expression library was constructed in the vectors EX1, pEX2, and pEX3 and screened with a pool of 19 well-absorbed sera from household contacts of leprosy patients. Twelve selected recombinants that were further characterized differed clearly from recombinants selected with murine monoclonal antibodies. Whereas the monoclonal antibodies recognized mainly six recombinant antigens, the human sera from contacts reacted with a range of different recombinant antigens. None of the contact recombinant antigens was identical or related to well-characterized antigens from M. leprae or other mycobacteria selected with monoclonal antibodies, including proteins of the heat shock families. Two groups of recombinant antigens could be distinguished: one that was recognized by all sera used in the pool and one that was recognized by only a limited number of sera. These antigens, selected with sera from household contacts of previously untreated lepromatous leprosy patients, may be relevant to the immune responses during the early phase of infection with M. leprae.

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DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation

Cited by 53 publications

References 24 publications

Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae

Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae

Geographically distinct isolates of Mycobacterium leprae exhibit no genotypic diversity by restriction fragment‐length polymorphism analysis

Selection and characterization of recombinant clones that produce Mycobacterium leprae antigens recognized by antibodies in sera from household contacts of leprosy patients

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