DNA logic assembly powered by a triplex-helix molecular switch for extracellular pH imaging

Qi, Hongjie; Yue, Shuzhen; Bi, Sai; Ding, Caifeng; Song, Weiling

doi:10.1039/c8cc04615h

Cited by 24 publications

(13 citation statements)

References 19 publications

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“…In addition to being genetic materials for life, DNA molecules following predictable Watson–Crick base-pairing rule have been widely used in the detection of proteins or cells using corresponding DNA aptamer. − Typically, after the recognition of DNA aptamer with a target, a short DNA strand is released which can transfer the detection of target to the detection of DNA. , However, because one target protein releases only one short DNA strand, the detection sensitivity is heavily restricted. Recently, various DNA nanomachines including DNA tweezers, DNA motors, DNA robots, and DNA walkers have been developed.…”

mentioning

confidence: 99%

Ultrasensitive Detection of Exosomes by Target-Triggered Three-Dimensional DNA Walking Machine and Exonuclease III-Assisted Electrochemical Ratiometric Biosensing

Sun

et al. 2019

Anal. Chem.

145

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Exosomes are membrane-enclosed phospholipid extracellular vesicles. In spite of their great promise as noninvasive biomarkers for cancer diagnosis, sensitive detection of exosomes is still challenging. Herein, the detection of exosomes was changed to the detection of DNA after recognition of exosomes with its aptamers. CD63 aptamer and EpCAM aptamer were used for the detection of MCF-7 cell-secreted exosome. The recognition process was amplified through the movements of a three-dimensional DNA walker. And then, Exonuclease III- assisted electrochemical ratiometric sensor was applied for further signal amplification. Under optimal conditions, the detection limit of 1.3 × 104 particles/mL was obtained with excellent selectivity. Furthermore, clinical application test for the detection of exosomes in human serum was also verified.

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mentioning

confidence: 99%

Ultrasensitive Detection of Exosomes by Target-Triggered Three-Dimensional DNA Walking Machine and Exonuclease III-Assisted Electrochemical Ratiometric Biosensing

Sun

et al. 2019

Anal. Chem.

145

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“…The inherent biocompatibility of DNA nanostructures also makes them highly attractive in drug delivery. In addition, a variety of functional DNA nanocarriers have been designed to controllably release payloads upon specific stimuli [170]. Moreover, chemical modifications on DNAs allow the addition of functional groups to DNA nanostructures, resulting in the construction of multifunctional therapeutic nanoplatforms in the applications of multimodal synergistic theranostics.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

DNA Nanotechnology for Multimodal Synergistic Theranostics

Qiao

Song

et al. 2021

J. Anal. Test.

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Over the past decade, DNA nanotechnology has developed rapidly due to its unique characteristics, such as excellent biocompatibility, high programmability, good predictability, automatically chemical synthesis, and so on. So far, a variety of DNA-based nanostructures, from small to large and simple to complex, have been designed and synthesized with controllable size and shape in one, two, or three dimensions. Therefore, DNA has become a kind of competitive materials for biosensing, bioimaging and biomedicine. In particular, the integration of DNA nanotechnology with multimodal synergistic theranostics can not only achieve accurate cancer diagnosis by the sensitive and accurate detection of cancer biomarkers, but also achieve enhanced anti-cancer therapeutic efficacy, which promote the development of DNA nanotechnology and nanomedicine. In this review, we first give a comprehensive introduction of DNA nanotechnology, and then summarize the DNA self-assembly and amplification strategies for the construction of functional nanoplatforms for multimodal synergistic theranostics. Finally, the challenges and opportunities faced by DNA nanotechnology in biomedicine are discussed.

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“…Similarly, nonconventional DNA interactions can be used to rationally design pH-sensitive DNA switches that can be used as nanometer scale pH meters. − Such probes typically exploit DNA secondary structures that display pH dependence due to the presence of specific protonation sites. These structures include I-motif, − inter- and intramolecular triplex DNA, − DNA tweezers, and catenanes . Recently, we have also reported on the rational design of programmable DNA-based nanoswitches whose closing and/or opening can be triggered over specific different pH windows by simply changing the relative content of TAT/CGC triplets in the switches .…”

Section: Dna Nanotechnology For Sensing Applicationsmentioning

confidence: 99%