DNA Logic Multiplexing Using Toehold-Mediated Strand Displacement

Abstract

Since the discovery of the DNA Strand Displacement mechanism, researchers have implemented a lot of applications, such as DNA computing, DNA Circuits, Logic gates, and Chemical Reaction network. To achieve those functions, a well-designed system is essential, among which the toehold domains and migration domains play a vital role. In this paper, we designed three basic logic gates based on the toehold mediates DNA strand displacement mechanism, and utilized them to struct a three-layer multiplexer DNA logic circuit. However, the traditional INHIBIT gate annihilated all the inputs strands which obscure the multiplexer in further applications. Therefore, we improved the INHIBIT gate, so the desired input strand can be selected, and the corresponding output strand can be identified. Lastly, we adjusted the multiplexer and realized a cyclic DNA circuit. The simulation results verified the efficiency and reliability of our multiplexer DNA logic circuits. Our method has the ability in architecting complex DNA integrated circuits.INDEX TERMS DNA strand displacement, logic circuits, INHIBIT gate, demultiplexer.

Rapid microRNA detection method based on DNA strand displacement for ovarian cancer cells

“…So far, TSDRs have been applied in various fields, including biocomputing systems [24][25][26][27], artificial neuro systems [28,29], bio-electronical circuits [30,31], and biosensors [32,33]. In the field of biosensors, many remarkable works have been accomplished.…”

Constructing DNA logic circuits based on the toehold preemption mechanism

“…[46][47][48] Compared with a monorail circuit, a dual-track circuit obviously increases the complexity of DNA strands which limits the expansion of DNA logic circuit. Second, the DNA strands used as inhibitory signals do not play a very specic or unique role in the DNA logic circuit, especially the XOR gate [49][50][51] in which the output suppression were realized by using complementary DNA strands as two different inputs. In this case, two complementary input DNA strands consume each other thus no output will be made.…”

DNA Logic Circuits Based on Accurate Step Function Gate

“…Many DNA logic gates have been proposed [22][23][24][25]. Fan et al constructed the DSD based digital switching circuits, and completed a full-adder logic.…”