DNA Marker Analysis of ‘Davis’ Soybean and Its Descendants for the<i>Rcs</i>3 Gene Conferring Resistance to<i>Cercospora sojina</i>

Missaoui, Ali; Phillips, D. V.; Boerma, H. R.

doi:10.2135/cropsci2006.07.0472

Cited by 15 publications

(10 citation statements)

References 21 publications

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“…These include 71 isolates from the United States, 15 isolates from Brazil, and 7 isolates from China (Table 1). In total, 93 C. sojina isolates have been analyzed for their reaction on 38 putative soybean differential cultivars (Missaoui et al, 2007). This research was conducted by various individuals over a long time period, and there was a wide range in the number of replications of the various C. sojina isolate–soybean differential evaluations.…”

Section: A New Classification Of C Sojina Isolatesmentioning

confidence: 99%

Frogeye Leaf Spot of Soybean: A Review and Proposed Race Designations for Isolates of Cercospora sojina Hara

et al. 2008

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Frogeye leaf spot (FLS), caused by Cercospora sojina K. Hara, is a common disease of soybean [Glycine max (L.) Merr.] in most soybean‐growing countries of the world. Significant yield losses of soybean (10–60%) have been attributed to FLS under hot and humid growing conditions. The FLS in the southern United States has been kept under control by planting resistant cultivars. Cercospora sojina is a dynamic pathogen with extensive virulence or race diversity. The current number of C. sojina isolates at the University of Georgia collection is 93. Assigning race designations to this large number of isolates has been difficult because of the lack of a well‐defined set of differentials. To facilitate future studies of the genetics of soybean resistance to FLS and the identification and comparison of existing and future races of C. sojina, we have reassessed both the soybean differentials and the C. sojina races. Based on the reactions of 93 isolates of C. sojina on 38 putative soybean differentials, we propose a core set of 12 differentials and 11 races that represent the major diversity among the 93 C. sojina isolates. The definition of these 12 differentials and 11 races should provide the foundation for the identification and comparison of additional soybean resistance genes and new races of C. sojina

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Section: A New Classification Of C Sojina Isolatesmentioning

confidence: 99%

Frogeye Leaf Spot of Soybean: A Review and Proposed Race Designations for Isolates of Cercospora sojina Hara

et al. 2008

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“…For five more lines, four of the five markers matched the haplotype of Davis, but in each case the marker not matching the Davis haplotype was one of the two markers closest to the Rcs3 gene. The Rcs3 is predicted to be located between AZ573CA150 and Satt‐244 (Missaoui et al, 2007b; Table 3). Remaining lines matched the Rcs3 haplotype of susceptible Blackhawk or had at least three of the five markers matching the haplotype of Blackhawk (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…This SSR marker has been successfully used for marker‐assisted selection (MAS) of this allele (Missaoui et al, 2007a). Recently, several single nucleotide polymorphism (SNP) and indel markers that are located within 3 cM of the Rcs3 allele have been identified, confirmed, and used for MAS for Rcs3 (Missaoui et al, 2007b). Marker‐assisted selection with SNP markers is most effective if several SNPs that are evenly spaced and closely linked to the gene of interest are used to define a haplotype of the genomic region flanking the gene (Johnson et al, 2001).…”

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Identification of Soybean Accessions Resistant to Cercospora sojina by Field Screening, Molecular Markers, and Phenotyping

et al. 2011

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Frogeye leaf spot (FLS) of soybean [Glycine max (L.) Merr.], caused by Cercospora sojina K. Hara, has been a problem in the southern United States for many years. Cultivars resistant to FLS have been developed for planting in this area, and resistance in many of these cultivars is conditioned by the Rcs3 alíele at the Rcs3 locus, which provides immunity to all known races of the pathogen. Frogeye leaf spot has recently become a greater problem in the northern United States, and few C. sojina resistant cultivars and breeding lines adapted to this area have been identified. The objectives of this study were to (i) identify maturity group (MG) 00 to VII accessions resistant to C. sojina race 11 by field screening at multiple locations over years and (ii) determine if FLS resistance in these accessions is likely to be conditioned by the Rcs3 alíele. A total of 522 accessions were evaluated for reaction to race 11 in field trials in Missouri and Illinois during 2006 and 2007, and 84 of these lines did not develop symptoms of FLS and were field screened for reaction to natural inoculum in Tennessee during 2008. Nine accessions of the 84 lines were susceptible.The remaining 75 were subsequently tested for the possible presence of Rcs3 using five molecular markers located within 2 cM of the gene. Of these 75 accessions, only PI 437726, PI 438302B, and PI 494851 had the Rcs3 haplotype of the cultivar Davis, the source of Rcs3. The soybean accessions predicted not to have the Rcs3 alíele and with no FLS symptoms in field trials may also be useful in developing soybean cultivars with resistance to FLS.A. Mengistu, USDA-ARS, 605 Airways Boulevard, Jackson, TN 38301; J. Bond,

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“…For PI 594891 and PI 594774, the haplotype windows were defined by the 72.6 kb fine-mapped region (Figs 1 and 2 ). The haplotype window for Davis was defined by the interval based on the primer sequence of Satt244) and AZ573TA150 markers used to map the Rcs3 gene on chr16 [ 30 ]. Therefore, each interval was based on the estimated physical locations of the SSR or SNP markers in the soybean genome map available at www.soybase.org using the Glyma1.01 map version.…”

Section: Methodsmentioning

confidence: 99%

Fine Mapping and Characterization of Candidate Genes that Control Resistance to Cercospora sojina K. Hara in Two Soybean Germplasm Accessions

et al. 2015

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Frogeye leaf spot (FLS), caused by the fungus Cercospora sojina K. Hara, may cause a significant yield loss to soybean growers in regions with a warm and humid climate. Two soybean accessions, PI 594891 and PI 594774, were identified to carry a high level of resistance similar to that conditioned by the Rcs3 gene in 'Davis'. Previously, we reported that the resistance to FLS in these two plant introductions (PIs) was controlled by a novel gene (s) on chromosome 13 that is different from Rcs3. To fine-map the novel FLS resistance gene(s) in these two PIs, F2: 3 seeds from the crosses between PI 594891 and PI 594774, and the FLS susceptible genotype 'Blackhawk' were genotyped with SNP markers that were designed based on the SoySNP50k iSelect BeadChip data to identify recombinant events and locate candidate genes. Analysis of lines possessing key recombination events helped narrow down the FLS-resistance genomic region in PI 594891 from 3.3 Mb to a 72.6 kb region with five annotated genes. The resistance gene in PI 594774 was fine-mapped into a 540 kb region that encompasses the 72.6 kb region found in PI 594891. Sequencing five candidate genes in PI 594891 identified three genes that have several mutations in the promoter, intron, 5', and 3' UTR regions. qPCR analysis showed a difference in expression levels of these genes in both lines compared to Blackhawk in the presence of C. sojina. Based on phenotype, genotype and haplotype analysis results, these two soybean accessions might carry different resistance alleles of the same gene or two different gene(s). The identified SNPs were used to develop Kompetitive Allele Specific PCR (KASP) assays to detect the resistance alleles on chromosome 13 from the two PIs for marker-assisted selection.

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DNA Marker Analysis of ‘Davis’ Soybean and Its Descendants for theRcs3 Gene Conferring Resistance toCercospora sojina

Cited by 15 publications

References 21 publications

Frogeye Leaf Spot of Soybean: A Review and Proposed Race Designations for Isolates of Cercospora sojina Hara

Frogeye Leaf Spot of Soybean: A Review and Proposed Race Designations for Isolates of Cercospora sojina Hara

Identification of Soybean Accessions Resistant to Cercospora sojina by Field Screening, Molecular Markers, and Phenotyping

Fine Mapping and Characterization of Candidate Genes that Control Resistance to Cercospora sojina K. Hara in Two Soybean Germplasm Accessions

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