DNA markers for identifying biotypes B and Q of <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae) and studying population dynamics

Khasdan, Vadim; Levin, Ilan; Rosner, Arie; Morin, Shai; Kontsedalov, Svetlana; Maslenin, Ludmila; Horowitz, A. R.

doi:10.1079/ber2005390

Cited by 138 publications

(117 citation statements)

References 35 publications

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“…Amplification of B. tabaci biotype specific band (816 bp) from the mtCO1 gene and characteristic band sizes from enzymatic digestion of the amplified fragment (Khasdan et al 2005) confirmed our hypothesis that biotype Q B. tabaci was present in Finland in the autumns of 2006 and 2008 on imported poinsettias (Table 1). We also detected biotype Q in 12 of the 13 poinsettia greenhouses representing different parts of Sweden in 2006.…”

Section: Resultssupporting

confidence: 69%

“…PCR amplification was performed using a PTC-200 DNA engine (MJ Research, Inc. Watertown, USA). The annealing temperature for primers C1J-2195 and L2-N-3014 was 52 °C, as recommended by Khasdan et al (2005). Aliquots of the PCR products were electrophoresed in a 1% agarose gel at 90 V for 1 h in 1 ¥ Tris-acetate-EDTA buffer (TAE) to determine the amplified PCR product.…”

Section: Modified By De La Rua Et Al (2006)mentioning

confidence: 99%

“…PCR products amplified with primer pairs C1-J-2195 and L2-N-3014 were digested with restriction endonuclease (VspI) (Promega) (Khasdan et al 2005) according to the manufacturer's instructions. Digested PCR products were electrophoresed in 1.5% agarose gel at 90 V for 1 h in 1 ¥ TAE buffer.…”

Section: Modified By De La Rua Et Al (2006)mentioning

confidence: 99%

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Bemisia tabaciBiotype Q Determined for the First Time on Poinsettia Crops in Finland and Sweden

Lemmetty

Vänninen

2014

Annales Zoologici Fennici

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We surveyed 13 Finnish and 13 Swedish poinsettia greenhouses in 2006 for Bemisia tabaci biotype Q (currently B. tabaci Mediterranean) and determined the biotype status of B. tabaci populations with PCR followed by an RFLP assay. We collected approximately 100 adult whiteflies from yellow sticky traps or from plants and stored them in 80% ethanol until DNA extraction. Total nucleic acids of two to five whiteflies per sample were extracted using FTA cards or a modified Chelex method. We showed that biotype Q was present in 10 out of 13 whitefly samples from Finnish poinsettia greenhouses and in 12 out of the 13 Swedish poinsettia greenhouses sampled. We also determined the whitefly biotypes from yellow sticky traps. The common occurrence of insecticide-resistance-prone biotype Q whiteflies on poinsettia imported into Finland and Sweden, both protected zones for B. tabaci, emphasizes the importance of preserving the quarantine status of the pest to prevent permanent establishment. Nonetheless, the increasing occurrence of a resistance-prone biotype on imported plant material will complicate preservation of the protected zone status if solely insecticides are used to control the pest.

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Section: Resultssupporting

confidence: 69%

Section: Modified By De La Rua Et Al (2006)mentioning

confidence: 99%

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Bemisia tabaciBiotype Q Determined for the First Time on Poinsettia Crops in Finland and Sweden

Lemmetty

Vänninen

2014

Annales Zoologici Fennici

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“…As técnicas moleculares mais utilizadas para identifi car os numerosos biótipos que formam o complexo B. tabaci e determinar as características genéticas distintas entre eles incluem PCR (Polymorphic Chain Reaction) (Calvert et al 2001, RAPD (Random Amplifi ed Polymorphic DNA) (Khasdan et al 2005, Rekha et al 2005, AFLP (Amplifi ed Fragment Length Polymorphism) (Cervera et al 2000, Göçmen & Devran 2002 e RFLP (Restriction Fragment Length Polymorphism) (Abdullahi et al 2004, Rabello 2005.…”

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Variabilidade genética de Bemisia tabaci (Gennadius) biótipo B (Hemiptera: Aleyrodidae) em cultivos olerícolas em São Luís, MA

Silva

Lemos

Lima³

et al. 2009

Neotrop. entomol.

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-The RAPD technique is widely used to investigate the distinct genetic characteristics of the complex Bemisia tabaci (Gennadius), which is currently constituted of approximately 41 biotypes. The objective of this research was to characterize populations of whitefl y collected in crops of agricultural producing areas in São Luís, MA, like okra, beans and pepper, using RAPD molecular markers. Females from nine whitefl y populations were analyzed and compared with B. tabaci biotype B taken from poinsettia culture of Embrapa Genetic Resources and Biotechnology (Brasília, DF). Twelve out of the 20 primers tested produced specifi c band patterns suitable to confi rm that the evaluated specimens belong to the biotype B of B. tabaci, despite the high percentage of detected polymorphism. The analysis of the 96 RAPD molecular markers generated indicated that the populations on okra, beans and pepper were grouped according to the host cultures, sharing 80, 76 and 45% of genetic similarity, respectively, when compared with the control population of B. tabaci biotype B. A lower selective pressure was observed with the population of whitefl y collected on pepper and minor genetic variability in the whitefl y populations collected on okra and bean, when compared with the control population.KEY WORDS: Whitefl y, host culture, RAPD, genetic characterization RESUMO -A técnica de RAPD é amplamente empregada para investigar características genéticas distintas dentro do complexo Bemisia tabaci Gennadius, atualmente constituído de aproximadamente 41 biótipos. O objetivo desta pesquisa foi caracterizar populações de mosca-branca coletadas em culturas agrícolas do município de São Luís, MA, como quiabo, feijão e pimentão, utilizando marcadores moleculares RAPD. Fêmeas de nove populações de mosca-branca foram analisadas e comparadas com o biótipo B de B. tabaci proveniente de cultura de poinsétia da Embrapa Recursos Genéticos e Biotecnologia (Brasília, DF). Dos 20 iniciadores utilizados, 12 produziram padrões de bandas específi cas, que permitiram confi rmar que os espécimes avaliados pertencem ao grupo do biótipo B de B. tabaci, apesar da alta percentagem de polimorfi smo detectado. Com os 96 marcadores moleculares RAPD gerados foi construído um dendrograma, que mostrou que as populações de quiabo, feijão e pimentão foram agrupadas de acordo com as culturas hospedeiras. A matriz de similaridade genética entre as populações de B. tabaci mostrou 80, 76 e 45% similaridade genética entre as populações das culturas de quiabo, feijão e pimentão, respectivamente, quando comparadas com a população controle de B. tabaci biótipo B. Foi também observada menor pressão de seleção na população de mosca-branca coletada em pimentão e menor variabilidade genética nas populações de mosca-branca coletadas em quiabo e feijão, quando comparadas com a população controle.

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“…The method of DNA extraction was done according to [18]. The leaf samples were crushed in liquid nitrogen and extracted in 50 ml extraction buffer (200 mM tris-HCl pH 8.5, 250 mM NaCl, 25 mM EDTA, 0.5% SDS) then sodium acetate (3M) (pH 5.2) was added.…”

Section: Dna Extractionmentioning

confidence: 99%

Epidemiology of Tomato Yellow Leaf Curl Virus in the Northern Regions of the West Bank, Palestine

Sawalha¹

2013

TOASJ

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Abstract:A survey was carried out in 2011 to study the epidemiology of tomato yellow leaf curl virus (TYLCV) in tomato growing sites of the Tobas and the Jenin districts. The survey studied the population of the virus-inoculative whiteflies and the possible virus reservoirs throughout the year. The maximum TYLCV-inoculative whitefly population was recorded in Tobas, compared with the Jenin district in the different growing seasons of tomato. In the Tobas district, the inoculative whiteflies started to appear in March and reached the maximum value of 7% in July and August. In the Jenin district, the maximum inoculative whitefly population of 6% was recorded in August. Such whiteflies started to appear in May, which is two months after their appearance in the Tobas district. Furthermore, populations of inoculative whiteflies occurred when farmers started their tomato growing seasons. The appearance of inoculative whiteflies coincided with availability of the virus natural reservoirs which harbor the virus and support the vector during the crop-free period. Infected cheese weed mallows (up to 12%) and tobacco plants (up to 7%) were the virus sources for whiteflies throughout the year in the Tobas and the Jenin districts respectively. Infected volunteer tomatoes (up to 93%) play a secondary role in the virus epidemiology as they disappear during winter (crop-free period) in both districts.

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DNA markers for identifying biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) and studying population dynamics

Cited by 138 publications

References 35 publications

Bemisia tabaciBiotype Q Determined for the First Time on Poinsettia Crops in Finland and Sweden

Bemisia tabaciBiotype Q Determined for the First Time on Poinsettia Crops in Finland and Sweden

Variabilidade genética de Bemisia tabaci (Gennadius) biótipo B (Hemiptera: Aleyrodidae) em cultivos olerícolas em São Luís, MA

Epidemiology of Tomato Yellow Leaf Curl Virus in the Northern Regions of the West Bank, Palestine

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