DNA-markers for resistance to common bunt transferred from Aegilops cylindrica Host. to hexaploid wheat

Abstract

Common bunt, caused by Tilletia caries (DC.) Tul., is widespread disease of wheat (Triticum aestivum L.) that causes serious yield reduction and loss of quality of this crop. Majority of Ukrainian wheat varieties are susceptible to bunt. It is necessary to search for sources of effective resistance genes.Aegilops cylindrica (2n = 28; genome CCDD) is a source of genes for some pest resistance in wheat Bochev et al. (1982). Ae. cylindrica carries resistance to leaf rust, stem rust, fusarium, septoria, powdery mildew and common bunt.The identification of DNA-markers linked to bunt resistance and susceptibility alleles would greatly facilitate the screening of wheat introgressive genotypes and accelerate the development of new resistant varieties. Microsatellites or simple sequence repeat (SSR) is widely used to search for molecular markers linked to many economic traits genes McIntosh et al. (2003).Present study reports markering and mapping with microsatellite markers of effective resistance gene to bunt in bread wheat introgressed from Ae. cylindrica. Bunt evaluation. Ae. cylindrica, recurrent parent wheat cv. Odesskaya polucarlikovaya and line Lutestens 23397, introgressive lines 5/55-91, 378/2000 and BC 3 F 2 -derived F 3 families were evaluated for resistance to bunt in field infection nursery. Bunt resistance in line 5/55-91 is controlled by a single dominant gene (Babayants et al. 2004). MATERIALS AND METHODS PlantMicrosatellite marker analysis. For SSR-analysis were used 95 pair primers to 107 microsatellite loci with known localization on chromosomes of wheat (Röder et al. 1998). For identification microsatellite markers linked to the resistance gene was used bulked segregation analysis (BSA) as described by Michelmore et al. (1991

Mapping of common bunt resistance gene Bt9 in wheat

“…In addition, introgressions of common bunt resistance factors from rye ( Secale cereale , Martynov and Dobrotvorskaya 2003), triticale (Ciuca 2011), barley ( Hordeum vulgare , Rubiales et al 2001), Aegilops glaucum (Martynov and Dobrotvorskaya 2003), Ae. cylindrica (Galaev et al 2006), Ae. ventricosa (Babayants et al 2006), Triticum erebuni (Babayants et al 2006) and Agropyron intermedium (Goates 1996) have been reported.…”

Location of genes for common bunt resistance in the European winter wheat cv. Trintella

“…In addition to these genes, 'alien' sources of resistance also have been used in breeding, for example, BtZ originating from Agropyron intermedium (Host) P. Beauv. (Goates 1996), or resistance derived from Aegilops cylindrica Host (Galaev et al 2006), and other grasses .…”

Population Structure and Genomewide Association Analysis of Resistance to Disease and Insensitivity to Ptr Toxins in Canadian Spring Wheat Using 90K SNP Array

“…Both the incidence and severity of common bunt have been controlled in the Canadian prairies, largely by introgressing resistance genes such as Bt10 and Bt8 (Menzies et al, 2006;McCallum and DePauw, 2008;Hiebert et al, 2011). Quantitative trait loci (QTLs) associated with resistance to common bunt have also been reported on chromosomes 1B and 7A (Galaev et al, 2006;Fofana et al, 2008;Wang et al, 2009). Resistance to common bunt has remained generally stable against shifts in virulence in the pathogen, but the vulnerability of such major genes through intense selection pressure on the pathogen is always a concern (Wang et al, 2009).…”

“…Resistance to common bunt has remained generally stable against shifts in virulence in the pathogen, but the vulnerability of such major genes through intense selection pressure on the pathogen is always a concern (Wang et al, 2009). Quantitative trait loci (QTLs) associated with resistance to common bunt have also been reported on chromosomes 1B and 7A (Galaev et al, 2006;Fofana et al, 2008;Wang et al, 2009). Tan spot, caused by P. tritici-repentis, is another economically important disease of wheat in Canada and other major wheat-growing countries (Faris et al, 1997;Friesen and Faris, 2004).…”